Fig 1: Genes upregulated upon BMP7 treatment in SC and DN derived differentiated adipocytes. SC and DN preadipocytes were differentiated and treated as in Figure 1, Figure 2, Figure 3, Figure 4, Figure 5 and Figure 6. (A) Volcano plot illustrating each of the upregulated genes in SC (red) and DN (blue) depots upon BMP7 treatment. (B) Venn-diagram showing the genes commonly up- or downregulated by BMP7 in SC and DN derived adipocytes. Gene expression of (C) ACAN, (D) CRYAB, and (E) ID1 as determined by RNA-sequencing (n = 9) and RT-qPCR (n = 5), followed by their respective protein expression (n = 5). Data presented as mean ± SD. RT-qPCR was normalized to GAPDH, protein expression was normalized to ß-actin. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Statistics: GLM (RNA-sequencing), one-way ANOVA with Tukey’s test (RT-qPCR and densitometry of western blots).