Description
For samples: This immunoassay kit allows for the in vitro semi-quantitative determination of human myelin oligodendrocyte glycoprotein antibody?IgG? concentrations in serum.
PRINCIPLE OF THE ASSAY The microtiter plate provided in this kit has been pre-coated with specificity antigen. Samples are then added to the appropriate microtiter plate wells and incubated. Then add Horseradish Peroxidase (HRP)-conjugated anti-human IgG and incubate again. Substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Calculate the valence of myelin oligodendrocyte glycoprotein antibody?IgG? in the samples