FITC anti-human CD4 317407 from BioLegend

Product Specs
ItemFITC anti-human CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number317407
NCBI Gene AliasesCD4mut, IMD79, Leu-3, OKT4D, T4
CloneOKT4
Conjugate/TagFITC
ApplicationsFlow Cytometry (FC)
IsotypeIgG2b, Kappa
HostMouse
Quantity25 tests
ReactivityHuman, Cynomolgus, Rhesus
Antibody TypePrimary
NCBI Full Gene NameCD4 molecule
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ClonalityMonoclonal
ImmunogenHuman peripheral T cells
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Transcriptional and chromatin profiling of human blood innate lymphoid cell subsets sheds light on HIV‐1 pathogenesisThe EMBO JournalAugust 15, 2023Yetao Wang, Lawrence Lifshitz, Noah J Silverstein, Esther Mintzer, Kevin Luk, Pamela StLouis, Michael A Brehm, Scot A Wolfe, Steven G Deeks, Jeremy LubanBiologics,https://www.newyorkbiologics.com Antibodies,Antibodies,Antibodies Anti‐Human CD3,BioLegend,"Cat# 317306 Clone: OKT3, FITC" Anti‐Human CD4,BioLegend,"Cat# 317408 Clone: OKT4, FITC" Anti‐Human TCRa/b,BioLegend,"Cat# 306706 Clone: IP26, FITC" Anti‐Human TCRg/d,BioLegend,"Cat#

(2) Influenza vaccine format mediates distinct cellular and antibody responses in human immune organoidsImmunityAugust 8, 2023Kastenschmidt JM, Sureshchandra S, Jain A, Hernandez-Davies JE, de Assis R, Wagoner ZW, Sorn AM, Mitul MT, Benchorin AI, Levendosky E, et al.CD4 (OKT4) BV650,Biolegend,cat # 317436; RRID: AB_2563050 Anti-Human CD4 (OKT4) FITC,Biolegend,cat # 317408; RRID: AB_571951 Anti-Human CD4 (OKT4) PE-Cy5,Biolegend,cat # 317412; RRID; AB_571957 Anti-Human CD45

(3) Human IRF1 governs macrophagic IFN-γ immunity to mycobacteriaCellFebruary 2, 2023Rosain J, Neehus AL, Manry J, Yang R, Le Pen J, Daher W, Liu Z, Chan YH, Tahuil N, Türel Ö, et al.(clone B1),BD Biosciences,"Cat# 555716, RRID:AB_396060" Human CD4 FITC (clone OKT4),BioLegend,"Cat# 317408, RRID:AB_571951" Human CD336 PerCP-eFluor710 (clone 44.189),Thermo Fisher Scientific,"Cat# 46-3369-42,

(4) Dynamically modeling the effective range of IL-2 dosage in the treatment of systemic lupus erythematosusiScienceSeptember 16, 2022Xin Gao, Jing He, Xiaolin Sun, Fangting LiAB_1645475. Percp-anti-human CD8,Biolegend,Cat. No. 344707; RRID: AB_1967122. FITC-anti-human CD4,Biolegend,Cat. No. 317408; RRID: AB_571951. BV421-anti-human CD25,Biolegend,Cat. No. 302630; RRID: AB_11126749. BV605-anti-human

(5) Alpha5 nicotinic acetylcholine receptor mediated immune escape of lung adenocarcinoma via STAT3/Jab1-PD-L1 signallingCell communication and signaling : CCSAugust 15, 2022Zhu P, Jin Z, Kang G, Jia Y, Liu D, Zhang Q, Guo F, Jia Y, Jiao Y, Li J, et al.1 ml media. Afterwards, primary T cells were incubated with CD4 antibody (1:20, BioLegend, Cat. No. 317408) and CD25 antibody (1:20, BioLegend, Cat. No. 302610) for 30 min. Samples were then fixed and permeabilized

(6) An anti-CTLA-4 heavy chain–only antibody with enhanced T _reg depletion shows excellent preclinical efficacy and safety profileProceedings of the National Academy of SciencesAugust 9, 2022Xin Gan et al.Generated iTreg cells were confirmed using a staining antibody mixture against human CD4 (Biolegend, 317408), CD25 (Biolegend, 356108), CTLA-4 (Biolegend, 349908), and FoxP3 (Biolegend, 320208). Pan-T cells were

(7) Single-cell profiling of immune cells after neoadjuvant pembrolizumab and chemotherapy in IIIA non-small cell lung cancer (NSCLC)Cell death & diseaseJuly 13, 2022Hui Z, Zhang J, Ren Y, Li X, Yan C, Yu W, Wang T, Xiao S, Chen Y, Zhang R, et al.(Brilliant Violet 510 anti-human CD45, Biolegend, Cat# 304035; FITC anti-human CD4, Biolegend, Cat# 317408; PE-Cy7 anti-human CD8, BD Biosciences, Cat# 557654; PE anti-human CD25, Biolegend, Cat# 302606; PerCP/Cy5.5 anti-human CD127(IL-7Rα

(8) Intensive single-cell analysis reveals immune-cell diversity among healthy individualsLife Science AllianceJuly 1, 2022Yukie Kashima et al.EBV (EBNA1, LMP1, and BZLF1) and incubated for 6 h in 37°C. For staining, we used FITC (CD4, Cat. no. 317408; BioLegend, RRID:AB_571951), PE (CD107a, Cat. no. 328608; BioLegend, RRID: AB_1186040), PerCP (CD8a,

(9) Single-cell multi-omics analysis reveals IFN-driven alterations in T lymphocytes and natural killer cells in systemic lupus erythematosusWellcome Open ResearchMay 12, 2022Dominik Trzupek, Mercede Lee, Fiona Hamey, Linda S. Wicker, John A. Todd, Ricardo C. FerreiraeBioscience; cat# 25-1278-42), CD4-FITC (Mouse anti-human monoclonal antibody, clone OKT4; Biolegend, cat# 317408), CD56-BV711 (Mouse anti-human monoclonal antibody, clone HCD56; Biolegend, cat# 318336), CD8-AF700

(10) Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivoSTAR ProtocolsMarch 18, 2022Deshan Ren, Wei Liu, Shuai Ding, Yan LiRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-human CD4 (Clone: OKT4) – FITC (1:50 dilution),BioLegend,Cat#: 317408 Anti-human CD19 (Clone: HIB19) – PE-CF594 (1:50 dilution),BD,Cat#: 562294 Anti-human CD38 (Clone:

(11) JAK and mTOR inhibitors prevent cytokine release while retaining T cell bispecific antibody in vivo efficacyJournal for ImmunoTherapy of CancerJanuary 21, 2022Gabrielle Leclercq et al.carbon dioxide. Flow cytometry At assay endpoint, PBMCs were stained with the antibodies to CD4 (FITC, 317408, Biolegend), CD8 (BV605, 344742, Biolegend), CD25 (BUV395, 564034, BD), CD69 (PE, 310306, Biolegend),

(12) Determination Factors Associated with Immune Responses Towards First Dose Edmonston-Zagreb (EZ) Measles Vaccine in Indonesian InfantsMedical ArchivesOctober 1, 2021Teni Teni Nurlatifah, Wisnu Barlianto, Wayan Arsana Wiyasa, Chandra Kusuma, Tita Luthfia Sari, Novilia Sjafri Bachtiarprotocol (21) Flow cytometry The cells suspension was labelled with FITC anti-human antibody (Biolegend:317408) and PE/Cy5 anti-human CD8a antibody (Biolegend:300910). Intracellular staining using PE Anti-human

(13) Stage-Dependent Within-Individual Comparison Reveals SIV-Specific Activation/Exhaustion Shift in Rhesus MacaquesFrontiers in MicrobiologyJuly 19, 2021Ling Tong, Zhe Cong, Long Tian, Jingjing Zhang, Jiahan Lu, Qiuhan Lu, Ting Chen, Yuhong Wang, Qiang Wei, Jing Xuewith monoclonal antibodies to CD3 PerCP (SP34-2, BD Biosciences, 552851), CD4 FITC (OKT-4, Biolegend, 317408), and CD8 PE (RPA-T8, BD Biosciences, 555367). CD4+ T-cell counts were determined with BD Trucount tubes

(14) Neoadjuvant chemoimmunotherapy in resectable stage IIIA/IIIB non-small cell lung cancerTranslational Lung Cancer ResearchJune 24, 2021Yulong Chen et al.purchased from BioLegend (San Diego, CA, USA). The following antibodies were used: FITC antihuman CD4 317408; PE anti-human CD25 302606; PE anti-human CD163 326506; PerCP/Cy5.5 anti-human CD127 (IL-7Rα) 351322;

(15) Measuring the effect of drug treatments on primary human CD8+ T cell activation and cytolytic potentialSTAR protocolsJune 18, 2021Loo Yau H, De Carvalho DD.(PE), 317306 (FITC), 317342; RRID AB_571913, AB_571907, AB_2563410" Anti-human CD4,BioLegend,Cat# 317408; RRID AB_571951 Anti-human CD8,BioLegend,"Cat# 344722 (APC), 344704 (FITC), 344714; RRID AB_2075388,

(16) Single-cell multi-omics analysis reveals IFN-driven alterations in T lymphocytes and natural killer cells in systemic lupus erythematosusWellcome Open ResearchJune 14, 2021Dominik Trzupek, Mercede Lee, Fiona Hamey, Linda S. Wicker, John A. Todd, Ricardo C. FerreiraeBioscience; cat# 25-1278-42), CD4-FITC (Mouse anti-human monoclonal antibody, clone OKT4; Biolegend, cat# 317408), CD56-BV711 (Mouse anti-human monoclonal antibody, clone HCD56; Biolegend, cat# 318336), CD8-AF700

(17) P32-specific CAR T cells with dual antitumor and antiangiogenic therapeutic potential in gliomasNature communicationsJune 14, 2021Rousso-Noori L, Mastandrea I, Talmor S, Waks T, Globerson Levin A, Haugas M, Teesalu T, Alvarez-Vallina L, Eshhar Z, Friedmann-Morvinski D.(Biolegend, Cat No. 300439, clone UCHT1, lot B278610, 5 μl per test). Anti-human CD4-FITC (Biolegend, Cat No. 317408, clone OKT4, lot B234953, 5 μl per test). Anti-human CD8a ef450 (Biolegend, Cat No. 48008842, clone

(18) Vaccination reshapes the virus-specific T cell repertoire in unexposed adultsImmunityJune 8, 2021Pan YG, Aiamkitsumrit B, Bartolo L, Wang Y, Lavery C, Marc A, Holec PV, Rappazzo CG, Eilola T, Gimotty PA, et al.Cat# 353208; RRID:AB_11203894 Mouse anti-human CD4,Biolegend,Cat# 344634; RRID:AB_2566017 or Cat# 317408; RRID:AB_571951 Mouse anti-human CD3,Biolegend,Cat# 300472; RRID:AB_2687178 Armenian hamster anti-human/mouse/rat

(19) Analysis of specific antibody and cellular immune response to first-dose measles vaccine Edmonston-Zagreb (EZ) in 9-month-old infantsAllergologia et ImmunopathologiaMay 1, 2021Teni Nurlatifah H.R., Wisnu Barlıanto, I Wayan Arsana Wıyasa, H M S Chandra Kusuma, Tita Luthfia Sari, Novilia Sjafri BachtıarIL-17A The cell suspensions were surface labeled with CD4 (FITC Anti-human CD4 Antibody (Biolegend, Cat.no. 317408) and CD8 antibodies (PE/Cy5 Anti-human CD8a Antibody) (Biolegend, Cat.no. 300910). Later, it was permeabilized for

(20) Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4⁺ T cellseLifeApril 9, 2021Cai J, Gao H, Zhao J, Hu S, Liang X, Yang Y, Dai Z, Hong Z, Deng K.reference,Identifiers,Additional information Antibody,Anti-human CD4-FITC (mouse monoclonal),Biolegend,Cat# 317408; RRID:AB_571951,Dilution 1:1000 Antibody,Anti-human CD8-APC (mouse monoclonal),Biolegend,Cat# 301049;

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11/18/2015Labeling of CD4 Positive Cells in Human PBMC Samples Max HübnerThis antibody gives quick staining, reliable results, great signal, and good value.

FITC anti-human CD4 from BioLegend

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FITC anti-human CD4 from BioLegend

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