Published customer image:
Mouse anti Rat CD4 antiboy, clone OX-68 (MCA1022R) used for the developments of an ELISA curve detailing procedures to describe biotin- streptavidin ratios. Protocol is described.
Image caption:
Establishing the ratios of biotinylated protein to streptavidin-PE using an ELISA-based method. An example of streptavidin-PE conjugation strategy used to generate an avid probe from a biotinylated monomeric protein. A dilution series of biotinylated monomers was incubated against a fixed concentration of streptavidin. The minimum dilution at which no excess biotinylated monomers can be detected was determined by ELISA. ELISA was performed with or without preincubating a range of protein dilutions with 10 ng of streptavidin-PE. In the presence of streptavidin-PE, the minimum dilution at which no signal was identified (circled black) and the amount of protein required for the saturation was calculated to generate a 10x stock solution with 4 μg/mL streptavidin-PE.

From: Sharma S, Wright GJ.
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens.
.
doi: 10.3791/60803.
This image is from an open access article distributed under terms of a.

Published customer image:
Mouse anti Rat CD4 antiboy, clone OX-68 (MCA1022R) used for the developments of an ELISA curve detailing procedures to describe biotin- streptavidin ratios. Protocol is described here.
Image caption:
Establishing the ratios of biotinylated protein to streptavidin-PE using an ELISA-based method. An example of streptavidin-PE conjugation strategy used to generate an avid probe from a biotinylated monomeric protein. A dilution series of biotinylated monomers was incubated against a fixed concentration of streptavidin. The minimum dilution at which no excess biotinylated monomers can be detected was determined by ELISA. ELISA was performed with or without preincubating a range of protein dilutions with 10 ng of streptavidin-PE. In the presence of streptavidin-PE, the minimum dilution at which no signal was identified (circled black) and the amount of protein required for the saturation was calculated to generate a 10x stock solution with 4 μg/mL streptavidin-PE.

From: Sharma S, Wright GJ.
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens.
J Vis Exp. 2020 Jun 6;(160).
doi: 10.3791/60803.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.