Fig 1: Effects of A6 and A15 on the viability of MCF-7 and SK-BR-3 cells transfected with siRNA targeting CA IX, II, or XII. MCF-7 and SK-BR-3 cells were transfected with control siRNA or siRNA targeting (A) CA IX, (B) CA II, and (C) CA XII. Western blotting analyses were performed as described in the Materials and Methods to confirm the efficiency of the respective knockdown. Representative blots are shown. Transfected cells were exposed to A6 or A15 at 50 µM for 48 h. Control cells were treated with the media containing 0.1% DMSO instead. Cell viability was determined by MTT assay as described in the Materials and Methods and expressed as a percentage of the control measured in the vehicle (DMSO)-treated cells transfected with control siRNA. Data are displayed as mean ± SEM from at least three independent experiments. *p < 0.05 vs. the vehicle-treated control cells transfected with control siRNA. ns, not significant.