GAD2 (D5G2) XP ® Rabbit mAb from Cell Signaling Technology

Product Specs
ItemGAD2 (D5G2) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5843S
Quantity100 µl
ApplicationsW, IP, IF-F
ReactivityH, M, R
TargetGAD2
HostRabbit
NCBI Gene AliasesGAD65
ClonalityMonoclonal
NCBI Full Gene Nameglutamate decarboxylase 2
Validated ApplicationsWB, IF, IP
Validated Species ReactivityHu, Ms, Rt
Research AreaNeuroscience
IsotypeIgG
CloneD5G2
Molecular Weight60

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Postpartum State, but Not Maternal Caregiving or Level of Anxiety, Increases Medial Prefrontal Cortex GAD65 and vGAT in Female RatsFrontiers in Global Women's HealthFebruary 8, 2022Christina M. Ragan, Eman I. Ahmed, Erika M. Vitale, Katrina Linning-Duffy, Stephanie M. Miller-Smith, Jamie Maguire, Joseph S. Lonsteinwas performed as described above but with a rabbit anti-GAD65 antiserum (1:2,000; Cell Signaling # 5843S) overnight at 4°C followed by incubation in a peroxidase-conjugated anti-rabbit IgG secondary antiserum

(2) Gephyrin-Lacking PV Synapses on Neocortical Pyramidal NeuronsInternational journal of molecular sciencesSeptember 17, 2021Kuljis DA, Micheva KD, Ray A, Wegner W, Bowman R, Madison DV, Willig KI, Barth AL.AB175,1:5000,AB_91011 GABAARα1,Mouse,NeuroMab 75–136,1:100,AB_2108811 GAD2,rabbit,Cell Signaling 5843,1:200,AB_10835855 Parvalbumin,rabbit,SWANT PV27,1:300,AB_2631173 Synapsin,rabbit,Cell Signaling 5297,1:200,AB_

(3) Chronic loss of inhibition in piriform cortex following brief, daily optogenetic stimulationCell reportsApril 20, 2021Ryu B, Nagappan S, Santos-Valencia F, Lee P, Rodriguez E, Lackie M, Takatoh J, Franks KM.Invitrogen N21483) at 4°C overnight. Staining for GAD2 (rabbit anti-GAD2, 1:500, Cell Signaling Technology 5843S) and somatostatin (rabbit anti-SST, 1:500, Invitrogen 701935) were done on the slices following the

(4) Amyloid Precursor Protein (APP) Controls the Expression of the Transcriptional Activator Neuronal PAS Domain Protein 4 (NPAS4) and Synaptic GABA ReleaseeneuroMay 1, 2020Rémi Opsomer et al.anti-microtubule associated protein 2 (MAP2; catalog #M4403, Sigma-Aldrich), anti-GAD65 (D5G2, catalog #5843 Cell Signaling), anti-mouse IgG, HRP whole antibody anti-rabbit IgG HRP (catalog #NA931-1ML, GE Healthcare),

(5) Endogenous Retrovirus Activation as a Key Mechanism of Anti-Tumor Immune Response in RadiotherapyRadiation researchApril 1, 2020Lee AK, Pan D, Bao X, Hu M, Li F, Li CY.IL)" TBK1,Western blot,"3013S, Cell Signaling Technologies (Danvers, MA)" Phospho-TBK1 (Ser172),Western blot,"5843S, Cell Signaling Technologies (Danvers, MA)" MAVS,Western blot,"4983S, Cell Signaling Technologies (Danvers,

(6) Multifaceted Changes in Synaptic Composition and Astrocytic Involvement in a Mouse Model of Fragile X SyndromeScientific ReportsDecember 1, 2019Anish K. Simhal, Yi Zuo, Marc M. Perez, Daniel V. Madison, Guillermo Sapiro, Kristina D. MichevaAB5905,RRID:AB_2301751,1:5000 VGluT2,Guinea pig,Millipore AB2251,RRID:AB_2665454,1:5000 GAD2,Rabbit,Cell Signaling 5843,RRID:AB_10835855,1:100 Gephyrin,Mouse,NeuroMab 75-443,RRID:AB_2636851,1:100 Glutamine synthetase,Mouse,BD Biosciences

(7) SSRIs target prefrontal to raphe circuits during development modulating synaptic connectivity and emotional behaviorMolecular psychiatryMay 1, 2019Soiza-Reilly M, Meye FJ, Olusakin J, Telley L, Petit E, Chen X, Mameli M, Jabaudon D, Sze JY, Gaspar P.AB2251), and anti-glutamate decarboxylase 65 (GAD2) (rabbit, 1:200; Cell Signaling Technology, USA; #5843) [32, 34]. The anti-synapsin 1 (rabbit, 1:400; Cell Signaling Technology, USA; #5297) was used to identify

(8) Ghrelin Causes a Decline in GABA Release by Reducing Fatty Acid Oxidation in CortexMolecular NeurobiologySeptember 1, 2018Joan Francesc Mir et al.membranes (Millipore). The following primary antibodies were used: GAD65/GAD2 (1/1000; Cell Signaling ref. 5843), VGLUT2 (1/1000; Cell Signaling ref. 71555), and β-actin (1/50,000; SigmaAldrich). Blots were incubated

(9) A Computational Synaptic Antibody Characterization Tool for Array TomographyFrontiers in NeuroanatomyJuly 17, 2018Anish K. Simhal, Belvin Gong, James S. Trimmer, Richard J. Weinberg, Stephen J Smith, Guillermo Sapiro, Kristina D. Micheva152 503,RRID:AB_2619850 Collybistin,Mouse,NeuroMab L120 project,RRID:AB_2650452 GAD2,Rabbit,Cell Signaling 5843,RRID:AB_10835855 Gephyrin BD,Mouse,BD Biosciences 612632,RRID:AB_399669 Gephyrin,Mouse,NeuroMab L106

(10) Ubiquitination of ABCE1 by NOT4 in Response to Mitochondrial Damage Links Co-translational Quality Control to PINK1-Directed MitophagyCell metabolismJuly 3, 2018Wu Z, Wang Y, Lim J, Liu B, Li Y, Vartak R, Stankiewicz T, Montgomery S, Lu B.ab68588), anti-P62 (PROGEN, GP62-C), anti-dP62/Ref2P (Abcam, ab-178440), anti-p-TBK1 (Cell Signaling, 5843S), anti-TBK1 (Cell Signaling, 3013S), anti-LC3B (Cell signaling, 2775S). Proximity ligation assay with

(11) Hexokinases link DJ-1 to the PINK1/parkin pathwayMolecular NeurodegenerationDecember 1, 2017David N. Hauser et al.ab13496, 1:1000), HSP60 (Abcam ab46798, 1:10,000), GLUL (Millipore MAB302, 1:40,000), GAD2 (Cell Signaling 5843S, 1:4000), PHGDH (Atlas Antibodies HPA021241, 1:4000), NEFL (Cell Signaling 2835S, 1:4000), HK1 (Cell Signaling

(12) Probabilistic fluorescence-based synapse detectionPLoS computational biologyApril 1, 2017Simhal AK, Aguerrebere C, Collman F, Vogelstein JT, Micheva KD, Weinberg RJ, Smith SJ, Sapiro G.AB175,RRID:AB_91011 Inhibitory,Presynaptic,vGAT,Mouse,Synaptic Systems 131-011,RRID:AB_887872 Inhibitory,Presynaptic,GAD,Rabbit,Cell Signaling Technology 5843,RRID:AB_10835855 Inhibitory,Postsynaptic,Gephyrin,Mouse,BD Biosciences 612632,RRID:AB_399669 Inhibitory,Postsynaptic,GABAARa1,Mouse,NeuroMab

(13) Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expressionScientific reportsFebruary 27, 2017Obora K, Onodera Y, Takehara T, Frampton J, Hasei J, Ozaki T, Teramura T, Fukuda K.Cell Signaling Technology, MA, USA. 1:2,000 dilution), mouse anti-Nestin monoclonal antibody (Rat-401, 14-5843, eBioscience. 1:1,000 dilution), mouse anti-Nf-M monoclonal antibody (RMO 14.9, 2838, Cell Signaling Technology.

(14) Collybistin binds and inhibits mTORC1 signaling: a potential novel mechanism contributing to intellectual disability and autismEuropean journal of human genetics : EJHGJanuary 1, 2016Machado CO, Griesi-Oliveira K, Rosenberg C, Kok F, Martins S, Passos-Bueno MR, Sertie AL.(1:1000, no. 9202), anti-eIF3H (1:1000, no. 3413), anti-PSD95 (1:1000, no. 3450), anti-GAD65 (1:1000, no. 5843), anti-pmTORS2448 (1:1000, no. 2971), anti-pS6K1T389 (1:1000, no. 9205), anti-prpS6S240/244 (1:5000,

(15) Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossingGenes & developmentDecember 15, 2015Hernandez-Enriquez B, Wu Z, Martinez E, Olsen O, Kaprielian Z, Maness PF, Yoshida Y, Tessier-Lavigne M, Tran TS.(R&D Systems, AF567), anti-Ben (1:100) (Dillon et al. 2005), rabbit anti-GAD65 (1:100; Cell Signaling, 5843), mouse IgM anti-4D7/TAG-1 (1:800; Developmental Studies Hybridoma Bank, clone 4D7), goat anti-TAG1

(16) Mapping synapses by conjugate light-electron array tomographyThe Journal of neuroscience : the official journal of the Society for NeuroscienceApril 8, 2015Collman F, Buchanan J, Phend KD, Micheva KD, Weinberg RJ, Smith SJ.ab8049 Millipore AB5905 Cell Signaling Technology 3450S Millipore MAB363 Millipore AB175 Cell Signaling Technology 5843 Biosciences Pharmingen 612632 Abcam ab18251 Sigma-Aldrich T6793 Abcam 41489 Sigma-Aldrich A8481 BD Biosciences

(17) Presynaptic gating of excitation in the dorsal raphe nucleus by GABAProceedings of the National Academy of SciencesSeptember 24, 2013Mariano Soiza-Reilly, Wayne B. Anderson, Christopher W. Vaughan, Kathryn G. Commonsmonoclonal antibodies for synapsin 1 and GAD2 (rabbit, 1:200; Cell Signaling Technologies; 5297 and 5843, respectively). Antibodies from different hosts were applied together in a random order and imaged,

(18) Neuropilin2 regulates the guidance of post-crossing spinal commissural axons in a subtype-specific mannerNeural developmentJuly 31, 2013Tran TS, Carlin E, Lin R, Martinez E, Johnson JE, Kaprielian Z.MN, USA) was used at 15 μg/ml, and a rabbit monoclonal anti-GAD2/GAD65 was used at 1:1000 (cat. no. 5843, Cell Signaling, Danvers, MA, USA) was used at 1:1000, with each antibody diluted in 10% donkey serum

(19) Structure and Ubiquitination-Dependent Activation of TANK-Binding Kinase 1Cell ReportsMarch 28, 2013Daqi Tu et al.Antibodies were obtained from Cell Signaling Technology (anti-TBK1 #3013, anti-phospho-TBK1 Ser172 #5843), Santa Cruz (anti-IRF3 #sc-9082), Millipore (anti-phospho-IRF3 Ser 396), and HA (Clone12C5) (Boehringer

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Frontiers in Global Women's Health

Frontiers in Global Women's Health

Frontiers in Global Women's Health

GAD2 (D5G2) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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GAD2 (D5G2) XP ® Rabbit mAb from Cell Signaling Technology

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