Propidium Iodide (PI)/RNase Staining Solution from Cell Signaling Technology

Product Specs
ItemPropidium Iodide (PI)/RNase Staining Solution
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number4087S
Quantity100 ml
Research AreaRelated Products
ApplicationsIF-F, IF-IC, FC-FP
ReactivityAll

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) CDK4/6 inhibition enhances SHP2 inhibitor efficacy and is dependent upon RB function in malignant peripheral nerve sheath tumorsScience AdvancesNovember 24, 2023Jiawan Wang, Ana Calizo, Lindy Zhang, James C. Pino, Yang Lyu, Kai Pollard, Xiaochun Zhang, Alex T. Larsson, Eric Conniff, Nicolas J. Llosa, et al.were then labeled with propidium iodide/ribonuclease staining solution (Cell Signaling Technology, no. 4087) and further incubated for 15 min at 37°C. Last, cells were analyzed using the FACSCelesta Cell Analyzer

(2) Characterization of the Inflammatory Response Evoked by Bacterial Membrane Vesicles in Intestinal Cells Reveals an RIPK2-Dependent Activation by Enterotoxigenic Escherichia coli VesiclesMicrobiology spectrumAugust 17, 2023Thapa HB, Kohl P, Zingl FG, Fleischhacker D, Wolinski H, Kufer TA, Schild S.(200 μL PI solution to 300 μL of serum free cell culture medium) (Cell Signaling, Inc.; catalog no. 4087S) for 5 min at room temperature prior to imaging. PI labels only cells with compromised plasma membranes

(3) Synthetic lethality of drug-induced polyploidy and BCL-2 inhibition in lymphomaNature communicationsMarch 18, 2023Portelinha A, da Silva Ferreira M, Erazo T, Jiang M, Asgari Z, de Stanchina E, Younes A, Wendel HG.were collected and then stained with propidium iodide (PI)/RNase staining solution (Cell Signaling, #4087) at room temperature for 15 min. Flow cytometric data were acquired on a BD FACSCalibur (BD Biosciences,

(4) Developing SHP2-based combination therapy for KRAS-amplified cancerJCI InsightFebruary 8, 2023Tianxia Li, Osamu Kikuchi, Jin Zhou, Yichen Wang, Babita Pokharel, Klavdija Bastl, Prafulla Gokhale, Aine Knott, Yanxi Zhang, John G. Doench, et al.were washed with PBS containing 1% FBS and then were stained with PI/RNase Staining Solution (catalog 4087; Cell Signaling Technology) for 30 minutes at room temperature. DNA content was measured by LSR II flow cytometer

(5) CDK4/6 inhibition enhances SHP2 inhibitor efficacy and is dependent upon restoration of RB function in malignant peripheral nerve sheath tumorsbioRxiv : the preprint server for biologyFebruary 3, 2023Wang J, Calizo A, Zhang L, Pino JC, Lyu Y, Pollard K, Zhang X, Larsson AT, Conniff E, Llosa N, et al.for at least 30 minutes and were then labeled with propidium iodide (PI)/RNase staining solution (#4087, Cell Signaling Technology) and further incubated for 15 minutes at 37°C. Finally, cells were analyzed

(6) Transcriptional Antagonism by CDK8 Inhibition Improves Therapeutic Efficacy of MEK InhibitorsCancer ResearchJanuary 18, 2023Clare F. Malone, Minjee Kim, Gabriela Alexe, Kathleen Engel, Alexandra B. Forman, Amanda Robichaud, Amy Saur Conway, Amy Goodale, Ashleigh Meyer, Delan Khalid, et al.fixed, permeabilized, and stained following the manufacturer’s instructions. Propidium Iodide (PI) (CST #4087) was used for DNA staining. Flow cytometry was performed on a BD FACSCelesta and analyzed using FlowJo

(7) Development of Combination Strategies for Focal Adhesion Kinase Inhibition in Diffuse Gastric CancerClinical Cancer ResearchJanuary 4, 2023Ke Peng, Feifei Zhang, Yichen Wang, Pranshu Sahgal, Tianxia Li, Jin Zhou, Xiaoyan Liang, Yanxi Zhang, Nilay Sethi, Tianshu Liu, et al.with PBS containing 1% FBS and stained with PI/RNase staining solution (Cell Signaling Technologies, 4087). The samples were incubated at room temperature for 30 min in the dark and then analyzed using a cell

(8) Inhibiting ERK5 Overcomes Breast Cancer Resistance to Anti-HER2 Therapy By Targeting the G1–S Cell-Cycle TransitionCancer Research CommunicationsDecember 5, 2022Jingwei Zhang, Adam J. Pearson, Nitin Sabherwal, Brian A. Telfer, Nisha Ali, Karmern Kan, Qiuping Xu, Wei Zhang, Fuhui Chen, Shiyang Li, et al.were incubated in 500 μL of propidium iodide (PI)/RNAse staining solution (Cell Signaling Technology #4087) according to the manufacturer's instructions. The cells were then resuspended in 400 μL of DPBS for

(9) Necroptosis-mediated HMGB1 secretion of keratinocytes as a key step for inflammation development in contact hypersensitivityCell Death DiscovNovember 7, 2022Ni Lian, Yujie Chen, Sihan Chen, Ta Xiao, Changjun Song, Yangying Ke, Xuecui Wei, Chunyan Gong, Hui Yu, Heng Gu, et al.Chemical Reagent (Shanghai, China), respectively. Propidium iodide (PI) / RNase staining solution (#4087 S) was purchased from Cell Signaling Technology (Danvers, MA, USA). In this study, some antibodies were

(10) Gain-of-function of TRPM4 predisposes mice to psoriasiform dermatitisFrontiers in ImmunologyOctober 20, 2022Daisuke Yamada, Simon Vu, Xuesong Wu, Zhenrui Shi, Desiree Morris, Joshua D. Bloomstein, Mindy Huynh, Jie Zheng, Samuel T. Hwangstained with anti-Ki-67 antibody (Cat# 151211, BioLegend) and propidium iodide containing RNase (Cat# 4087, Cell Signaling Technologies). IMQ-induced PsD model IMQ-induced PsD model was described previously

(11) TP53 mutations and RNA-binding protein MUSASHI-2 drive resistance to PRMT5-targeted therapy in B-cell lymphomaNature CommunicationsSeptember 27, 2022Tatiana Erazo et al.and then stained with propidium iodide (PI)/RNase staining solution (Cell Signaling Technology, cat.4087) at room temperature for 15 minutes. Flow cytometric data were acquired using a FACSCalibur (BD Biosciences,

(12) Integrated PPI- and WGCNA-retrieval of hub gene signatures for soft substrates inhibition of human fibroblasts proliferation and differentiationAging (Albany NY)September 14, 2022Ziran Xu, Tian Zhou, Yin Wang, Leijie Zhu, Jihao Tu, Zhixiang Xu, Lisha Li, Yulin Liand discarded and hFFs are washed with PBS for 3 times, 5 min/time. After that, the PI/RNase mixture (4087S, CST) is added and incubated for 15~20 min in the dark. Lastly, the reaction solution is discarded after

(13) N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cellsCell death & diseaseJuly 19, 2022Wang R, Wen P, Yang G, Feng Y, Mi Y, Wang X, Zhu S, Chen YQ.PBS, cells were stained with propidium iodide (PI) containing RNase (PI/RNase Staining Solution, CST 4087, MA, US) for 15 min in the dark, and then subjected to cell cycle analysis on a flow cytometer. The

(14) ALKBH5 regulates somatic cell reprogramming in a phase-specific mannerJournal of Cell ScienceJune 1, 2022Sherif Khodeer, Arne Klungland, John Arne Dahland the pellet was suspended in 200 µl propidium iodide (PI)/RNase Staining Solution (Cell signaling 4087S) and incubated at room temperature for 30 min before analysis by FACS. Apoptosis Detection of apoptotic

(15) Novel Small Molecules Capable of Blocking mtRAS-Signaling PathwayFrontiers in OncologyDecember 9, 2021Namkyoung Kim et al.with 70% EtOH for 1 h at −20°C. Cells were stained with PI/RNase staining solution (Cell signaling, #4087) and incubated 30 min at room temperature in a dark condition. Stained cells were subjected to flow cytometry analysis

(16) Worenine reverses the Warburg effect and inhibits colon cancer cell growth by negatively regulating HIF-1αCellular & Molecular Biology LettersDecember 1, 2021Lijiang Ji et al.under an inverted microscope. Cell cycle analysis Propidium iodide (PI) RNase staining solution (4087S, CST) was used for the cell cycle assay. HCT-116 cells were incubated with worenine for 24 h. Afterwards,

(17) Anti-Tumor Activity of AZD4547 Against NTRK1 Fusion Positive Cancer Cells Through Inhibition of NTRKsFrontiers in OncologyNovember 1, 2021Hanna Cho, Namkyoung Kim, Takashi Murakami, Taebo SimThermo Fisher Scientific. For use in cell cycle analysis, propidium iodide in a RNase containing solution (#4087) was obtained from Cell Signaling Technology. Primary antibodies including phospho-TRKA/B, phospho-AKT

(18) ACE2 protein expression within isogenic cell lines is heterogeneous and associated with distinct transcriptomesScientific reportsAugust 5, 2021Sherman EJ, Emmer BT.cells were incubated for 10 min on ice with Propidium Iodide Cell Staining Solution (Cell Signaling #4087) prior to flow cytometry. Microscopy Cells were seeded in 35 mm poly-D lysine-coated glass bottom

(19) ASN007 is a selective ERK1/2 inhibitor with preferential activity against RAS-and RAF-mutant tumorsCell Reports MedicineJuly 20, 2021Ana Portelinha, Scott Thompson, Roger A. Smith, Mariana Da Silva Ferreira, Zahra Asgari, Andrea Knezevic, Venkatraman Seshan, Elisa de Stanchina, Sandeep Gupta, Louis Denis, et al.were collected and then stained with propidium iodide (PI)/RNase staining solution (Cell Signaling, #4087) at room temperature for 15 minutes. Flow cytometric data was acquired on a BD FACSCalibur (BD Biosciences,

(20) RAB38 Facilitates Energy Metabolism and Counteracts Cell Death in Glioblastoma CellsCellsJune 30, 2021Elena Bianchetti, Sierra J. Bates, Trang T. T. Nguyen, Markus D. Siegelin, Kevin A. Roth85% ethanol for 4 h and then stained with propidium iodide (PI) solution (PI/RNAse Staining Solution, 4087, Cell Signaling, Danvers, MA, USA) at room temperature for 15 min. Fluorescence was measured using a

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Propidium Iodide (PI)/RNase Staining Solution from Cell Signaling Technology

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Propidium Iodide (PI)/RNase Staining Solution from Cell Signaling Technology

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