Fig 1: NaBut modulates the repair proteins in transformed cells. (a) Immunoblotting with antibodies to repair proteins using normal (MEF, NIH3T3) and transformed (E1A+Ras) cells untreated (-) or treated with 4 mM NaBut for 48 h. Immunoblotting with a-tubulin antibodies were used as a loading control. I—hyperphosphorylated Mre11, II—hypophosphorylated Mre11, III—truncated Mre11. (b) The bar plots represent band densities, evaluated using Image J © soft, scaled to the loading control, and normalized to the band density in untreated MEF cells (folds). For Ku80 and Mre11 proteins, the relevant plots display the aggregated intensity of all detected bands at each specific point, both for untreated and NaBut-treated cells. (c) Immunoblotting with antibodies to Mre11 using E1A+Ras cells untreated (-) or treated with 2.5 µM wortmannin (WM), 20 µM LY294002 (LY), or 10 mM NaBut for 8 h. GAPDH expression was used as a loading control. (d) Immunoblotting with antibodies to Mre11 using E1A+Ras cells untreated (-) or treated with NaBut for 24–48 h. Lanes 1–3 represent total cell extracts (total CE), and lanes 4–6 represent extracts co-precipitated with Rad50 antibodies.