Pacific Blue(TM) anti-human CD4 300521 from BioLegend

Product Specs
ItemPacific Blue(TM) anti-human CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number300521
NCBI Gene AliasesCD4mut, IMD79, Leu-3, OKT4D, T4
CloneRPA-T4
Quantity100 µg
IsotypeIgG1, Kappa
HostMouse
ApplicationsFlow Cytometry (FC)
Conjugate/TagPacific Blue
ReactivityHuman
Antibody TypePrimary
NCBI Full Gene NameCD4 molecule
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) In Vitro 3D Spheroid Culture System Displays Sustained T Cell-dependent CLL Proliferation and SurvivalHemaSphereSeptember 1, 2023Marco V. Haselager, Bianca F. van Driel, Eduard Perelaer, Dennis de Rooij, Danial Lashgari, Remco Loos, Arnon P. Kater, Perry D. Moerland, Eric Eldering(Franklin Lakes, NJ)" Anti-human CD3,345767,"BD Biosciences (Franklin Lakes, NJ)" Anti-human CD4,300524,"BioLegend (San Diego, CA)" Anti-human CD20,302320,"BioLegend (San Diego, CA)" Flow cytometry,Flow cytometry

(2) Phase III Pivotal comparative clinical trial of intranasal (iNCOVACC) and intramuscular COVID 19 vaccine (Covaxin®)NPJ VaccinesAugust 18, 2023Chandramani Singh, Savita Verma, Prabhakar Reddy, Michael S. Diamond, David T. Curiel, Chintan Patel, Manish Kumar Jain, Sagar Vivek Redkar, Amit Suresh Bhate, Vivek Gundappa, et al.fluorescently conjugated antibodies obtained from Biolegend, USA: CD3-APC-A750 (Cat. 300470), CD4-PB450 (Cat. 300521), CD8a-APC-A700 (Cat. 301028), OX40-APC (Cat. 350008), CD137-ECD (Cat. 309826), CD69-PC7 (Cat. 310912),

(3) Serpin B9 controls tumor cell killing by CAR T cellsJournal for ImmunoTherapy of CancerMarch 17, 2023Thomas Kimman, Anne Slomp, Anne Martens, Sarah Grabherr, Shuang Li, Eline van Diest, Jan Meeldijk, Jurgen Kuball, Monique C Minnema, Eric Eldering, et al.prior to adding effector cells. CD20 CAR T cells were characterized by staining with CD4-Pacific Blue (#300521, BioLegend) (RPA-T4), CD8-PE/Cy7 (#335822, BD Biosciences) (SK1), and biotinylated protein L (#M00097,

(4) Dominant-negative signal transducer and activator of transcription (STAT)3 variants in adult patients: A single center experienceFrontiers in ImmunologyDecember 20, 2022Oded Shamriz, Limor Rubin, Amos J. Simon, Atar Lev, Ortal Barel, Raz Somech, Maya Korem, Sigal Matza Porges, Tal Freund, David Hagin, et al.phosphorylation assay, PBMCs were stained with anti-CD4 (Pacific Blue clone RPA-T4; BioLegend Cat #300521) before being stimulated with either IL-10 (10n g/mL; Miltenyi Biotec) or IL-21 (100 ng/mL; Miltenyi

(5) Persistence of immunity and impact of third dose of inactivated COVID-19 vaccine against emerging variantsScientific ReportsDecember 1, 2022Krishna Mohan Vadrevu et al.fluorescently conjugated antibodies obtained from Biolegend, USA: CD3-APC-A750 (300470), CD4-PB450 (300521), CD8a-APC-A700 (301028), OX40-APC (350008), CD137-ECD (309826), CD69-PC7 (310912), CCR7-PE (353204),

(6) CD38 reduces mitochondrial fitness and cytotoxic T cell response against viral infection in lupus patients by suppressing mitophagyScience advancesJune 17, 2022Chen PM, Katsuyama E, Satyam A, Li H, Rubio J, Jung S, Andrzejewski S, Becherer JD, Tsokos MG, Abdi R, et al.antibodies or reagents, listed with clones, vendor, and catalog numbers: anti-human CD4 (clone RPA-T8, 300521), anti-human CD8a (clone SK1, BioLegend, 344718), anti-human CD8a (clone HIT8a, BioLegend, 300908),

(7) Preexisting and Post–COVID-19 Immune Responses to SARS-CoV-2 in Patients with CancerCancer DiscoveryAugust 1, 2021Tatjana Bilich et al.using Cytofix/Cytoperm solution (BD), Pacific Blue anti-human CD4 (1:100 dilution, BioLegend, Cat# 300524, RRID: AB_493099), FITC anti-human CD8 (1:100 dilution, BioLegend, Cat# 300905, RRID: AB_314908), PE

(8) IL-17A/F enable cholangiocytes to restrict T cell-driven experimental cholangitis by upregulating PD-L1 expressionJournal of HepatologyApril 1, 2021Stephanie Stein et al.IL-17A,PE,eBio17B7,eBioscience,12–7177-81 anti-human CD3,AF700,UCHT1,Biolegend,300424 anti-human CD4,PacificBlue,RPA-T4,Biolegend,300521 anti-human IL17A,AF647,BL168,Biolegend,512310 anti-human IFNgamma,PE,4S.B3,Biolegend,502509 Murine

(9) Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing SpondylitisFrontiers in immunologyJanuary 1, 2021Lee YJ, Kim MJ, Jo S, Jin SH, Park PR, Park K, Song HC, Kim J, Kim JY, Shim SC, et al.(100 ng/ ml) and ionomycin (1 mM) for 4 h, cells were stained with Paciﬁc Blue-conjugated anti-CD4 (300521, BioLegend, USA), and PEconjugated anti-CD45RO (304205, BioLegend, USA). Cells were washed, ﬁxed, permeabilized

(10) Different immunological effects of the molecular targeted agents sunitinib, everolimus and temsirolimus in patients with renal cell carcinomaInternational journal of oncologyApril 1, 2020Kobayashi Y, Yamada D, Kawai T, Sato Y, Teshima T, Yamada Y, Nakamura M, Suzuki M, Matsumoto A, Nakagawa T, et al.BioLegend, Inc.), CD4 (cat. no. 300512; BioLegend, Inc.), CD4 (cat. no. 300514; BioLegend, Inc.), CD4 (cat. no. 300521; BioLegend, Inc.), CD4 (cat. no. 300538; BioLegend, Inc.), CD8 (cat. no. 300926; BioLegend, Inc.), CD11b

(11) The receptor for advanced glycation endproducts (RAGE) modulates T cell signalingPloS oneJanuary 1, 2020Reed JC, Preston-Hurlburt P, Philbrick W, Betancur G, Korah M, Lucas C, Herold KC.(25/MEK1, 20 μl, Cat#560101), from Biolegend (San Diego CA): Pacific Blue-anti-human CD4 (RPAT4, 5 μl, Cat#300524), BV650- anti-human CD8 (RPAT8, 5 μl, Cat#301042), Phycoerythrin (PE) anti-Lck Phospho (Tyr394) Antibody (A18002D,

(12) Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examplesJournal of immunological methodsDecember 1, 2019van der Velden VHJ, Flores-Montero J, Perez-Andres M, Martin-Ayuso M, Crespo O, Blanco E, Kalina T, Philippé J, Bonroy C, de Bie M, et al.

(13) Endogenous CD4⁺ T Cells Recognize Neoantigens in Lung Cancer Patients, Including Recurrent Oncogenic KRAS and ERBB2 (Her2) Driver MutationsCancer immunology researchJune 1, 2019Veatch JR, Jesernig BL, Kargl J, Fitzgibbon M, Lee SM, Baik C, Martins R, Houghton AM, Riddell SR.capture and detection reagents), as well as with anti-CD4–pacific blue (clone RPA 14, Biolegend cat. 300521) and anti CD8–FITC (clone HIT8a, BD pharmigen cat. 555634) on a FACS Aria2 (BD Biosciences). Sorted

(14) Influence of nasal polyp tissue on the differentiation and activation of T lymphocytes in a co-culture systemBiomedical reportsFebruary 1, 2019Ickrath P, Scherzad A, Kleinsasser N, Ginzkey C, Hagen R, Hackenberg S.

(15) Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitisInternational journal of molecular medicineAugust 1, 2018Ickrath P, Kleinsasser N, Ding X, Ginzkey C, Beyersdorf N, Hagen R, Kerkau T, Hackenberg S.

(16) Identification of a human intestinal myeloid cell subset that regulates gut homeostasisInternational immunologyNovember 1, 2016Barman S, Kayama H, Okuzaki D, Ogino T, Osawa H, Matsuno H, Mizushima T, Mori M, Nishimura J, Takeda K.

(17) Reduced serpinB9-mediated caspase-1 inhibition can contribute to autoinflammatory diseaseOncotargetApril 12, 2016Robert van der Burgh, Jan Meeldijk, Lieneke Jongeneel, Joost Frenkel, Niels Bovenschen, Mariëlle van Gijn, Marianne Boes[11]. Antibodies FACS: CD3-APC, CD8-PerCP-Cy5 (BD biosciences, 555335 & 341050) CD4-PB (Biolegend, 300521). Western blot: Actin (Santa Cruz, sc-1616). Anti-FLAG (clone M2, Sigma Aldrich, F3165). Secondary antibodies

(18) Eradication of B-ALL using chimeric antigen receptor-expressing T cells targeting the TSLPR oncoproteinBloodJuly 30, 2015Qin H, Cho M, Haso W, Zhang L, Tasian SK, Oo HZ, Negri GL, Lin Y, Zou J, Mallon BS, et al.

(19) Pre-Vaccination Frequencies of Th17 Cells Correlate with Vaccine-Induced T-Cell Responses to Survivin-Derived Peptide EpitopesPloS oneJanuary 1, 2015Køllgaard T, Ugurel-Becker S, Idorn M, Andersen MH, Becker JC, Straten PT.

(20) A preliminary evaluation of the effects of opioids on innate and adaptive human in vitro immune functionBMJ supportive & palliative careDecember 1, 2014Boland JW, Foulds GA, Ahmedzai SH, Pockley AG.

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1/11/2016Very Good Staining of CD4+ T Cells Isabell BaumannThe aim of this study was a phenotypic analysis of human peripheral blood of children using minimal amount of sample. Therefore a reliable staining of major leukocytes subsets such as CD4+ T cells is ... Read Review

Pacific Blue(TM) anti-human CD4 from BioLegend

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Pacific Blue(TM) anti-human CD4 from BioLegend

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