Fig 1: IgG2b and IgG2c are the predominant subclasses of FLT3-specific IgG induced by VRP-FLT3 vaccine. Antigen-specific IgG1 (A), IgG2b (B), IgG2c (C), and IgG3 (D) responses were detected by ELISA using the recombinant FLT3 as the capture antigen. (A–D) Titration curves of samples was shown. One sample in PBS and VRP-Ctrl groups was tested, and ten samples in VRP-FLT3 groups were tested. (E) Levels of anti-FLT3 IgG subclasses were compared by calculating the equivalent IgG subclass concentration using O.D. values at 1:300 dilution of anti-FLT3 IgG1 and IgG3 ELIS as indicated as the vertical dash line in (A) and (D), and O.D. values at 1:2700 dilution of anti-FLT3 IgG2b and IgG2c indicated as the vertical line in (B, C) ELISAs. Each symbol represents an individual mouse (n=10). Bars represent the mean ± SD. Statistical significance were determined by one-way ANOVA, *P<0.05, **P<0.01, ***P<0.001. Data represents one of two independent experiments.