Anti-LAMP1 antibody [H4A3] ab25630 from Abcam

Product Specs
ItemAnti-LAMP1 antibody [H4A3]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab25630
Antibody TypePrimary
Quantity100 UG
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
CloneH4A3
IsotypeIgG1
ClonalityMonoclonal
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration0.1 mg/ml
Research AreaImmuno-oncology
Gene NameLAMP1
ReactivityHuman
TargetLAMP1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) HIV-1-induced nuclear invaginations mediated by VAP-A, ORP3, and Rab7 complex explain infection of activated T cellsNature communicationsAugust 10, 2023Santos MF, Rappa G, Karbanová J, Diana P, Cirrincione G, Carbone D, Manna D, Aalam F, Wang D, Vanier C, et al.EPR24395-31; ICC (1:100)) rabbit monoclonal antibodies were purchased from Abcam (#ab8982, #ab63958, #ab25630, #ab137029, #ab252919, and #ab278043, respectively). Anti-GAPDH rabbit polyclonal antibody (IB (1:1000))

(2) A genome-wide atlas of human cell morphologybioRxivAugust 7, 2023Meraj Ramezani, Julia Bauman, Avtar Singh, Erin Weisbart, John Yong, Maria Lozada, Gregory P. Way, Sanam L. Kavari, Celeste Diaz, Marzieh Haghighi, et al.cells were fixed, permeabilized, blocked, and stained as above, using primary antibody against LAMP1 (ab25630, Abcam; 1:50) and Alexa Fluor 647-conjugated secondary antibody. Alexa Fluor 555-conjugated WGA at 1.5

(3) Homotypic SCOTIN assemblies form ER-endosome membrane contacts and regulate endosome dynamicsEMBO reportsAugust 3, 2023Yun H, Jung M, Lee H, Jung S, Kim T, Kim N, Park SY, Kim WJ, Mun JY, Yoo JY.anti‐EEA1 (Cell Signaling, C45B10), mouse anti‐CD63 (BD Pharmingen, 556019), mouse anti‐LAMP1 (Abcam, ab25630), rabbit anti‐TfR (Abcam, ab84036), and FITC anti‐Myc (Abcam, ab1263). Then, secondary antibodies conjugated

(4) Autophagy Inhibition via Hydroxychloroquine or 3-Methyladenine Enhances Chemotherapy-Induced Apoptosis in Neuro-Blastoma and GlioblastomaInternational Journal of Molecular SciencesJuly 27, 2023Darcy Wear, Eesha Bhagirath, Arpana Balachandar, Caleb Vegh, Siyaram Pandeyfor 1 h at room temperature with the following primary antibodies: LAMP1 (mouse IgG, 1:250, Cat. No. ab25630) (Abcam Inc., Cambridge, UK), LC3B (rabbit IgG, 1:500, Cat. No. ab192890) (Abcam Inc.), 4-HNE (rabbit

(5) SCARB1 downregulation in adrenal insufficiency with Allgrove syndromeOrphanet Journal of Rare DiseasesJune 19, 2023Giacomo Bitetto, Gianluca Lopez, Dario Ronchi, Alessandra Pittaro, Valentina Melzi, Erika Peverelli, Fulvia Milena Cribiù, Giacomo P. Comi, Giovanna Mantovani, Alessio Di Fonzoantibodies: Anti-AAAS TA808612 (Origene) mouse (1:1000), Actin A2066 (Sigma) rabbit (1:1200), Lamp1 AB25630 (Abcam) mouse (1:800), Lamin a/c 2032S (Cell signaling) rabbit (1:800–1000), α-tubulin 3873S (Cell signaling)

(6) Interferon-inducible phospholipids govern IFITM3-dependent endosomal antiviral immunityThe EMBO journalMay 15, 2023Unali G, Crivicich G, Pagani I, Abou-Alezz M, Folchini F, Valeri E, Matafora V, Reisz JA, Giordano AMS, Cuccovillo I, et al.Sino Biological Cat#204314‐T08), mouse anti‐LAMP1 monoclonal antibody (1:100 dilution from Abcam [H4A3] (ab25630)), goat anti‐LAMP1(1:100 dilution from Abcam cat#ab2245). After three washes with 1× PBS, cells were

(7) The exocyst complex is an essential component of the mammalian constitutive secretory pathwayJournal of Cell BiologyMay 1, 2023Conceição Pereira, Danièle Stalder, Georgina S.F. Anderson, Amber S. Shun-Shion, Jack Houghton, Robin Antrobus, Michael A. Chapman, Daniel J. Fazakerley, David C. Gershlickanti-ELKS antibody (EPR13777; 1:5,000; ab180507; Abcam), mouse anti-LAMP1 antibody (H4A3; 1:10,000; ab25630; Abcam), rabbit anti-TIMP2 antibody (D18B7; 1:1,000; 5738; Cell Signaling Technology), rabbit anti-Cystatin

(8) Proteomic Analysis of Huntington's Disease Medium Spiny Neurons Identifies Alterations in Lipid DropletsMolecular & cellular proteomics : MCPMay 1, 2023Tshilenge KT, Aguirre CG, Bons J, Gerencser AA, Basisty N, Song S, Rose J, Lopez-Ramirez A, Naphade S, Loureiro A, et al.(SQSTM1) (Abcam, ab56416, 1:100), rabbit anti-LC3 (Novus, Nb100-2331, 1:100), mouse anti-LAMP1 (Abcam, ab25630, 1:100). The secondary antibodies were donkey anti-rabbit, anti-mouse IgG conjugated with Alexa-488

(9) Comprehensive Membrane N-Glycoproteomics Using Human Breast Cancer Cell Line PairsMass Spectrom (Tokyo)April 11, 2023Daisuke Takakura, Haruka Yoshida, Shoko Ohashi, Nana Kawasakiwith the primary antibodies in 5% albumin in PBS for 2 h at 37°C. Primary antibodies against LAMP1 (ab25630, Abcam, Cambridge, UK) and cathepsin D (#2284, Cell Signaling Technology, Danvers, MA, USA) were diluted

(10) Common and Rare Variants in TMEM175 Gene Concur to the Pathogenesis of Parkinson's Disease in Italian PatientsMolecular neurobiologyApril 1, 2023Palomba NP, Fortunato G, Pepe G, Modugno N, Pietracupa S, Damiano I, Mascio G, Carrillo F, Di Giovannantonio LG, Ianiro L, et al.LC3I/II, p62, LAMP1, TFEB, GAPDH, and PARP1 was determined by immunoblot analysis using anti-LAMP1 (ab25630, Abcam, Cambridge, UK, 1:1000), anti-LC3B (2775, Cell Signaling, Danvers, Massachusetts, USA, 1:1000/),

(11) The synthetic TRPML1 agonist, ML-SA1, rescues Alzheimer-related alterations of the endosomal-autophagic-lysosomal systemJournal of Cell ScienceMarch 15, 2023Aleksandra Somogyi, Emily D. Kirkham, Emyr Lloyd-Evans, Jincy Winston, Nicholas D. Allen, John J. Mackrill, Karen E. Anderson, Phillip T. Hawkins, Sian E. Gardiner, Helen Waller-Evans, et al.#610456, 1:400), anti-LAMP1 (D2D11) (Cell Signaling, USA, #9091, 1:50), anti-LAMP1 (H4A3) (Abcam, UK, ab25630 1:50); anti-LC3 (Cell Signaling, #2775, 1:250), anti-p-Ser396/404 tau (PHF1, a generous gift from Dr

(12) A Pore Forming Toxin-like Protein Derived from Chinese Red Belly Toad Bombina maxima Triggers the Pyroptosis of Hippomal Neural Cells and Impairs the Cognitive Ability of MiceToxinsMarch 3, 2023Ye Q, Wang Q, Lee W, Xiang Y, Yuan J, Zhang Y, Guo X.purchased from Sigma-Aldrich (St. Louis, MO, USA). The anti-GSDME (ab215191), anti-Lamp1 antibody (ab25630), anti-GSDMD (ab219800), and anti-IL-18 (ab191860) antibodies were purchased from Abcam Inc. (Cambridge,

(13) StARD9 is a novel lysosomal kinesin required for membrane tubulation, cholesterol transport and Purkinje cell survivalJournal of cell scienceMarch 1, 2023Sterling FR, D'Amico J, Brumfield AM, Huegel KL, Vaughan PS, Morris K, Schwarz S, Joyce MV, Boggess B, Champion MM, et al.Ipswich, MA). Antibodies against NPC1 (5 μg/ml for IF, 1 μg/ml for WB, ab108921), LAMP1 (1:10 for IF, ab25630), SREBP1 (1:100 for IF, ab28481) and cathepsin B (1 μg/ml for IF, ab6313) were obtained from Abcam (Boston,

(14) Involvement of Mitochondrial Dysfunction in FOXG1 SyndromeGenesJanuary 17, 2023Bjerregaard VA, Levy AM, Batz MS, Salehi R, Hildonen M, Hammer TB, Møller RS, Desler C, Tümer Z.chemiluminescence meter. Primary antibodies used were: SOD2 (1:1000, D9V9C; Cell Signaling), LAMP1 (1:200, H4A3, ab25630; Abcam), LC3B-II (1:2000, NB600-1384SS, Novus Biologicals, Englewood, CO, USA), and GAPDH (1:2000, MA5-15738;

(15) Exploring therapeutic strategies for infantile neuronal axonal dystrophy (INAD/PARK14)eLifeJanuary 16, 2023Guang Lin, Burak Tepe, Geoff McGrane, Regine C Tipon, Gist Croft, Leena Panwala, Amanda Hope, Agnes J H Liang, Zhongyuan Zuo, Seul Kee Byeon, et al.(Glycobiotech; RAS_0010); mouse anti-ATP5a (Abcam; ab14748; RRID:AB_301447); mouse anti-LAMP1 (Abcam; ab25630; RRID:AB_470708); goat anti-Vps35 (Abcam; ab10099; RRID: AB_296841); mouse anti-GFP FITC conjugated

(16) Misrouting of glucagon and stathmin-2 towards lysosomal system of α-cells in glucagon hypersecretion of diabetesIsletsDecember 31, 2022Farzad Asadi, Savita Dhanvantarimarkers Rab11A (Cat# ab180778, Abcam; 1:100) and Rab11B (Cat# ab 228954, Abcam;1:100) and Lamp1 (Cat # ab25630, Abcam; 1:50) Then, corresponding secondary antibodies used were donkey anti-goat IgG Alexa Fluor 555

(17) Comparative proteomic analysis of children FSGS FFPE tissuesBMC PediatricsDecember 12, 2022Jiajia Ni, Sha Tian, Lin Bai, Qianying Lv, Jialu Liu, Jiaojiao Liu, Ye Fang, Yihui Zhai, Qian Shen, Jia Rao, et al.serum in PBS for an hour at room temperature, tissues were stained with an anti-LAMP-1 antibody (Abcam, ab25630; dilution 1:25) and anti-ACSL4 antibody (Abcam, ab155282; dilution 1:250). Afterwards, secondary antibody

(18) The dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistanceNature communicationsDecember 6, 2022Dall'Agnese A, Platt JM, Zheng MM, Friesen M, Dall'Agnese G, Blaise AM, Spinelli JB, Henninger JE, Tevonian EN, Hannett NM, et al.ab62352, 1:500 dilution), anti-clathrin (Abcam, ab24578, ab62352, 1:500 dilution), anti-LAMP1 (Abcam, ab25630, ab62352, 1:500 dilution), and anti-EEA1 (Abcam, ab70521, ab62352, 1:500 dilution), anti-pIRS1 (Abcam,

(19) Airway Smooth Muscle Cell Mitochondria Damage and Mitophagy in COPD via ERK1/2 MAPKInternational journal of molecular sciencesNovember 12, 2022Fang L, Zhang M, Li J, Zhou L, Tamm M, Roth M.,BD Bioscience,556432,Mouse,1:1000 MTCO2,Abcam,Ab79393,Rabbit,1:1000 PGC-1α,Abcam,ab54481,Rabbit,1:500 GAPDH,Abcam,ab128915,Rabbit,1:5000 LAMP-1,Abcam,ab25630,Mouse,1:500 EEA1,Abcam,ab70521,Mouse,1:500 Collagen I,Abcam,ab34710,Rabbit,1:1000 α-SMA,Abcam,ab124964,Rabbit,1:5000 Fibronectin,Thermo-Fisher,MA5-11981,Mouse,1:500 Anti-Ms-Alexa-488,Thermo-Fisher,A21121,Goat,1:500 Anti-Rab-Alexa-546,Thermo-Fisher,A11010,Goat,1:500 Anti-mouse-HRP,Sigma-Aldrich,A9917,Goat,1:2000 Anti-rabbit-HRP,Sigma-Aldrich,A9169,Goat,1:2000 Antibody

(20) Mycobacterium tuberculosis Utilizes Host Histamine Receptor H1 to Modulate Reactive Oxygen Species Production and Phagosome Maturation via the p38MAPK-NOX2 AxismBioOctober 26, 2022Mo S, Guo J, Ye T, Zhang X, Zeng J, Xu Y, Peng B, Dai Y, Xiao W, Zhang P, et al.with anti-HRH1 (Immunoway, 1:100) and anti-LAMP-1 (lysosomal associated membrane protein 1, Abcam, ab25630, 1:200) antibodies followed by incubation for 1 h at RT with a fluorophore-conjugated secondary antibody

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Anti-LAMP1 antibody [H4A3] from Abcam

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Anti-LAMP1 antibody [H4A3] from Abcam

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