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Anti-LAMP1 antibody [H4A3]
Anti-LAMP1 antibody [H4A3] from Abcam
Be the first to write a review!
Citations:
(222)
Supplier Page
Supplier Page from
Abcam for
Anti-LAMP1 antibody [H4A3]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-LAMP1 antibody [H4A3]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab25630
Antibody Type
Primary
Quantity
100 UG
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Clone
H4A3
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse, Nude mouse
Conjugate/Tag
Unconjugated
Concentration
0.1 mg/ml
Research Area
Immuno-oncology
Gene Name
LAMP1
Reactivity
Human
Target
LAMP1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (222)
(1)
Development of a cationic polyethyleneimine-poly(lactic-co-glycolic acid) nanoparticle system for enhanced intracellular delivery of biologics†
RSC Advances
November 16, 2023
Shannon R. Tracey, Peter Smyth, Una M. Herron, James F. Burrows, Andrew J. Porter, Caroline J. Barelle, Christopher J. Scott
primary antibody:
goat
anti-
mouse
anti-lysosomal-associated membrane protein 1 (LAMP1) antibody (
Abcam
(
ab25630
);
1 : 50
dilution in blocking buffer) or
goat
anti-rabbit EEA1 antibody (cell signalling technology
(2)
Tau forms synaptic nano-biomolecular condensates controlling the dynamic clustering of recycling synaptic vesicles
Nature Communications
November 10, 2023
Shanley F. Longfield, Mahdie Mollazade, Tristan P. Wallis, Rachel S. Gormal, Merja Joensuu, Jesse R. Wark, Ashley J. van Waardenberg, Christopher Small, Mark E. Graham, Frédéric A. Meunier,
et al.
monoclonal antibody (
1:200
,
Abcam
, #
ab176333
), anti-Lamp1
mouse
monoclonal antibody (
1:100
,
Abcam
, #
ab25630
), anti-GFP chicken polyclonal antibody (
1:1000
,
MERK
, #
AB16901
), anti-
Tau
mouse
monoclonal antibody
(3)
Ataxia-Telangiectasia Mutated Is Involved in Autolysosome Formation
Biomolecules & Therapeutics
September 1, 2023
Mihwa Hwang, Dong Wha Jun, Bo Ram Song, Hanna Shim, Chang-Hun Lee, Sunshin Kim
Alternatively, the fixed cells were incubated with PBST-BSA containing anti-LAMP1 antibody (
1:500
,
ab25630
,
Abcam
, Cambridge, UK) overnight at 4°C, followed by Alexa Fluor® 594-conjugated anti-
mouse
antibodies
(4)
HIV-1-induced nuclear invaginations mediated by VAP-A, ORP3, and Rab7 complex explain infection of activated T cells
Nature communications
August 10, 2023
Santos MF, Rappa G, Karbanová J, Diana P, Cirrincione G, Carbone D, Manna D, Aalam F, Wang D, Vanier C,
et al
.
EPR24395-31;
ICC
(
1:100
)) rabbit monoclonal antibodies were purchased from
Abcam
(#
ab8982
, #
ab63958
, #
ab25630
, #
ab137029
, #
ab252919
, and #ab278043, respectively). Anti-GAPDH rabbit polyclonal antibody (IB (
1:1000
))
(5)
A genome-wide atlas of human cell morphology
bioRxiv
August 7, 2023
Meraj Ramezani, Julia Bauman, Avtar Singh, Erin Weisbart, John Yong, Maria Lozada, Gregory P. Way, Sanam L. Kavari, Celeste Diaz, Marzieh Haghighi,
et al.
cells were fixed, permeabilized, blocked, and stained as above, using primary antibody against LAMP1 (
ab25630
,
Abcam
;
1:50
) and Alexa Fluor 647-conjugated secondary antibody. Alexa Fluor 555-conjugated WGA at 1.5
(6)
Homotypic SCOTIN assemblies form ER-endosome membrane contacts and regulate endosome dynamics
EMBO reports
August 3, 2023
Yun H, Jung M, Lee H, Jung S, Kim T, Kim N, Park SY, Kim WJ, Mun JY, Yoo JY.
anti‐EEA1 (Cell Signaling, C45B10), mouse anti‐CD63 (BD
Pharmingen
,
556019
), mouse anti‐LAMP1 (
Abcam
,
ab25630
), rabbit anti‐TfR (
Abcam
,
ab84036
), and FITC anti‐
Myc
(
Abcam
,
ab1263
). Then, secondary antibodies conjugated
(7)
A superoxide-driven redox state promotes geroconversion and resistance to senolysis in replication-stress associated senescence
Redox Biology
August 1, 2023
Le Luo, Shazib Pervaiz, Marie-Veronique Clement
Reagents Primary antibodies used: 53BP1 (Cat. #
SC-22760
;
Santa Cruz Biotechnology
) and LAMP1 (Cat. #
ab25630
;
Abcam
). Primary antibodies obtained from
Cell Signaling Technology
:
Ki-67
(Cat. #
9129
), phospho-histone
(8)
Autophagy Inhibition via Hydroxychloroquine or 3-Methyladenine Enhances Chemotherapy-Induced Apoptosis in Neuro-Blastoma and Glioblastoma
International Journal of Molecular Sciences
July 27, 2023
Darcy Wear, Eesha Bhagirath, Arpana Balachandar, Caleb Vegh, Siyaram Pandey
for 1 h at room temperature with the following primary antibodies: LAMP1 (mouse IgG,
1:250
, Cat. No.
ab25630
) (
Abcam
Inc., Cambridge, UK),
LC3B
(rabbit IgG,
1:500
, Cat. No.
ab192890
) (
Abcam
Inc.), 4-HNE (rabbit
(9)
Dysregulation of Histone Deacetylases Inhibits Trophoblast Growth during Early Placental Development Partially through TFEB-Dependent Autophagy-Lysosomal Pathway
International Journal of Molecular Sciences
July 25, 2023
Peixin Wang, Chenqiong Zhao, Hanjing Zhou, Xiaona Huang, Hanqi Ying, Songying Zhang, Yibin Pan, Haiyan Zhu
AcH3K27 (
8171S
;
1:1000
;
CST
, Peachtree City, GA, USA);
TFEB
(
ab267351
;
1:1000
;
Abcam
, USA); LAMP1 (
ab25630
;
1:1000
;
Abcam
, USA);
P21
(
2947S
;
1:1000
;
CST
, USA); CyclinB1 (
12231S
;
1:1000
;
CST
, USA); Caspase3 (
9662S
;
(10)
SCARB1 downregulation in adrenal insufficiency with Allgrove syndrome
Orphanet Journal of Rare Diseases
June 19, 2023
Giacomo Bitetto, Gianluca Lopez, Dario Ronchi, Alessandra Pittaro, Valentina Melzi, Erika Peverelli, Fulvia Milena Cribiù, Giacomo P. Comi, Giovanna Mantovani, Alessio Di Fonzo
antibodies: Anti-AAAS
TA808612
(
Origene
)
mouse
(
1:1000
), Actin
A2066
(
Sigma
) rabbit (
1:1200
), Lamp1
AB25630
(
Abcam
)
mouse
(
1:800
), Lamin a/c
2032S
(
Cell signaling
) rabbit (
1:800
–1000), α-tubulin
3873S
(
Cell signaling
)
(11)
Interferon-inducible phospholipids govern IFITM3-dependent endosomal antiviral immunity
The EMBO journal
May 15, 2023
Unali G, Crivicich G, Pagani I, Abou-Alezz M, Folchini F, Valeri E, Matafora V, Reisz JA, Giordano AMS, Cuccovillo I,
et al
.
Sino Biological
Cat#
204314‐T08
), mouse anti‐LAMP1 monoclonal antibody (
1:100
dilution from
Abcam
[H4A3] (
ab25630
)),
goat
anti‐LAMP1(
1:100
dilution from
Abcam
cat#
ab2245
). After three washes with 1× PBS, cells were
(12)
Microtubule-associated protein MAP1LC3C regulates lysosomal exocytosis and induces zinc reprogramming in renal cancer cells
Journal of Biological Chemistry
May 1, 2023
Rita Verma, Parul Aggarwal, Megan E. Bischoff, James Reigle, Dina Secic, Collin Wetzel, Katherine VandenHeuvel, Jacek Biesiada, Birgit Ehmer, Julio A. Landero Figueroa,
et al.
saponin and 1% BSA for 30 min and incubated with primary mouse anti-LAMP1 antibody (
Abcam
; catalog no.:
ab25630
,
1:3000
dilution) or rabbit anti-CTSD antibody (
Abcam
; catalog no.:
ab6313
,
1:2000
dilution) for 1 h
(13)
Proteomic Analysis of Huntington's Disease Medium Spiny Neurons Identifies Alterations in Lipid Droplets
Molecular & cellular proteomics : MCP
May 1, 2023
Tshilenge KT, Aguirre CG, Bons J, Gerencser AA, Basisty N, Song S, Rose J, Lopez-Ramirez A, Naphade S, Loureiro A,
et al
.
(SQSTM1) (
Abcam
,
ab56416
,
1:100
), rabbit anti-
LC3
(
Novus
,
Nb100-2331
,
1:100
), mouse anti-LAMP1 (
Abcam
,
ab25630
,
1:100
). The secondary antibodies were
donkey
anti-rabbit, anti-mouse IgG conjugated with Alexa-488
(14)
The exocyst complex is an essential component of the mammalian constitutive secretory pathway
Journal of Cell Biology
May 1, 2023
Conceição Pereira, Danièle Stalder, Georgina S.F. Anderson, Amber S. Shun-Shion, Jack Houghton, Robin Antrobus, Michael A. Chapman, Daniel J. Fazakerley, David C. Gershlick
anti-ELKS antibody (EPR13777
;
1:5,000
;
ab180507
;
Abcam
), mouse anti-LAMP1 antibody (H4A3;
1:10,000
;
ab25630
;
Abcam
), rabbit anti-TIMP2 antibody (D18B7;
1:1,000
;
5738
;
Cell Signaling Technology
), rabbit anti-Cystatin
(15)
Comprehensive Membrane
N
-Glycoproteomics Using Human Breast Cancer Cell Line Pairs
Mass Spectrom (Tokyo)
April 11, 2023
Daisuke Takakura, Haruka Yoshida, Shoko Ohashi, Nana Kawasaki
with the primary antibodies in 5% albumin in PBS for 2 h at 37°C. Primary antibodies against LAMP1 (
ab25630
,
Abcam
, Cambridge, UK) and cathepsin D (#
2284
,
Cell Signaling Technology
, Danvers, MA, USA) were diluted
(16)
Common and Rare Variants in TMEM175 Gene Concur to the Pathogenesis of Parkinson's Disease in Italian Patients
Molecular neurobiology
April 1, 2023
Palomba NP, Fortunato G, Pepe G, Modugno N, Pietracupa S, Damiano I, Mascio G, Carrillo F, Di Giovannantonio LG, Ianiro L,
et al
.
LC3I/II,
p62
, LAMP1,
TFEB
,
GAPDH
, and PARP1 was determined by
immunoblot
analysis using anti-LAMP1 (
ab25630
,
Abcam
, Cambridge, UK,
1:1000
), anti-
LC3B
(
2775
,
Cell Signaling
, Danvers, Massachusetts, USA,
1:1000
/),
(17)
The synthetic TRPML1 agonist, ML-SA1, rescues Alzheimer-related alterations of the endosomal-autophagic-lysosomal system
Journal of Cell Science
March 15, 2023
Aleksandra Somogyi, Emily D. Kirkham, Emyr Lloyd-Evans, Jincy Winston, Nicholas D. Allen, John J. Mackrill, Karen E. Anderson, Phillip T. Hawkins, Sian E. Gardiner, Helen Waller-Evans,
et al.
#
610456
,
1:400
), anti-LAMP1 (D2D11) (
Cell Signaling
, USA, #
9091
,
1:50
), anti-LAMP1 (H4A3) (
Abcam
, UK,
ab25630
1:50
); anti-LC3 (
Cell Signaling
, #
2775
,
1:250
), anti-p-Ser396/404 tau (PHF1, a generous gift from Dr
(18)
A Pore Forming Toxin-like Protein Derived from Chinese Red Belly Toad Bombina maxima Triggers the Pyroptosis of Hippomal Neural Cells and Impairs the Cognitive Ability of Mice
Toxins
March 3, 2023
Ye Q, Wang Q, Lee W, Xiang Y, Yuan J, Zhang Y, Guo X.
purchased from
Sigma-Aldrich
(St. Louis, MO, USA). The anti-GSDME (
ab215191
), anti-Lamp1 antibody (
ab25630
),
anti-GSDMD
(
ab219800
), and anti-
IL-18
(
ab191860
) antibodies were purchased from
Abcam
Inc. (Cambridge,
(19)
StARD9 is a novel lysosomal kinesin required for membrane tubulation, cholesterol transport and Purkinje cell survival
Journal of cell science
March 1, 2023
Sterling FR, D'Amico J, Brumfield AM, Huegel KL, Vaughan PS, Morris K, Schwarz S, Joyce MV, Boggess B, Champion MM,
et al
.
Ipswich, MA). Antibodies against
NPC1
(5 μg/ml for IF, 1 μg/ml
for WB
,
ab108921
), LAMP1 (
1:10
for IF,
ab25630
), SREBP1 (
1:100
for IF,
ab28481
) and cathepsin B (1 μg/ml for IF,
ab6313
) were obtained from
Abcam
(Boston,
(20)
Autolysosomal activation combined with lysosomal destabilization efficiently targets myeloid leukemia cells for cell death
Frontiers in Oncology
February 1, 2023
Harshit Shah, Metodi Stankov, Diana Panayotova-Dimitrova, Amir Yazdi, Ramachandramouli Budida, Jan-Henning Klusmann, Georg M. N. Behrens
,
Cell Signaling
,USA
Anti-rabbit IgG-HRP Linked
,
7074s
,
Cell Signaling
,USA
Atg7
,
8558s
,
Cell Signaling
,USA Lamp-1,
ab25630
,
Abcam
,UK
Lamp-2
,
sc-18822
,
Santa Cruz Biotechnology
,USA
p62
/
SQSTM1
,
7695s
,
Cell Signaling
,USA p4E-BP1,
2855s
,
Cell Signaling
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Anti-LAMP1 antibody [H4A3] from Abcam
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Product Specs
Company Info
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