Anti-LAMP1 antibody [1D4B] ab25245 from Abcam

Product Specs
ItemAnti-LAMP1 antibody [1D4B]
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab25245
Antibody TypePrimary
ApplicationsAffinity purification, Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Quantity100 UG
ClonalityMonoclonal
IsotypeIgG2a
Clone1D4B
HostRat
Conjugate/TagUnconjugated
Concentration0.5 mg/ml
Research AreaImmuno-oncology
Gene NameLAMP1
ReactivityHuman
TargetLAMP1

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Cathepsin D overexpression in the nervous system rescues lethality and Aβ42 accumulation of cathepsin D systemic knockout in vivoActa Pharmaceutica Sinica BOctober 1, 2023Xiaosen Ouyang, Willayat Y. Wani, Gloria A. Benavides, Matthew J. Redmann, Hai Vo, Thomas van Groen, Victor M. Darley-Usmar, Jianhua Zhang(Santa Cruz SC-13985), anti-NeuN (Millipore MAB377), anti-GFAP (Dako Z033429), anti-LAMP1 [1D4B] (Abcam ab25245), anti-LAMP2 antibody (Abcam AB37024), α-synuclein (Invitrogen 328200), p-α-synuclein (Covance MMS-5091-100),

(2) Sirt6 enhances macrophage lipophagy and improves lipid metabolism disorder by regulating the Wnt1/β-catenin pathway in atherosclerosisLipids in Health and DiseaseSeptember 22, 2023Tingting Wang, Zheng Cheng, Ran Zhao, Jin Cheng, He Ren, Pengke Zhang, Pengyun Liu, Qimeng Hao, Qian Zhang, Xiaolei Yu, et al.(ab15251; Abcam), Sirt6 (ab191385; Abcam), β-catenin (ab32572; Abcam), GAPDH (ab181602; Abcam), LAMP1 (ab25245; Abcam), PLIN2 (ab108323; Abcam) and Adipophilin (ab108323; Abcam). A chemiluminescence system (Amersham

(3) Lysosome blockade induces divergent metabolic programs in macrophages and tumours for cancer immunotherapyJournal of experimental & clinical cancer research : CRAugust 4, 2023Ma J, Ma R, Zeng X, Zhang L, Liu J, Zhang W, Li T, Niu H, Bao G, Wang C, et al.PBS containing 0.1% Tween-20 for 30 min, cells were incubated overnight at 4 °C with Lamp1 (Abcam, ab25245, 1: 500), and Lamp2 (Abcam, ab25339, 1: 100), p65 (Cell Signaling Technology, #8242, 1: 400), in 2%

(4) Apolipoprotein E intersects with amyloid-β within neuronsLife Science AllianceAugust 1, 2023Sabine C Konings, Emma Nyberg, Isak Martinsson, Laura Torres-Garcia, Oxana Klementieva, Claudia Guimas Almeida, Gunnar K GourasAB_10917271 Rabbit polyclonal anti-LAMP1,Abcam,Cat#ab24170; RRID: AB_775978 Rat monoclonal anti-LAMP1,Abcam,Cat# ab25245; RRID: AB_449893 Rabbit polyclonal anti-LC3β,Cell signaling,Cat#2775; RRID: AB_915950 Chicken polyclonal

(5) Complement Drives Chronic Inflammation and Progressive Hydrocephalus in Murine Neonatal Germinal Matrix HemorrhageInternational Journal of Molecular SciencesJune 15, 2023Mohammed Alshareef, Devin Hatchell, Tyler Vasas, Khalil Mallah, Aakash Shingala, Jonathan Cutrone, Ali Alawieh, Chunfang Guo, Stephen Tomlinson, Ramin EskandariUSA. Cat. #: 14-5921-82, 1:400), anti-MBP (Abcam, Cat. #: ab40390, 1:150), anti-LAMP-1 (Abcam, Cat. #: ab25245, 1:200), and anti-GFAP (Invitrogen, Cat. #: 13-0300, 1:200). The secondary antibodies utilized were

(6) Lipid nanoparticle delivery limits antisense oligonucleotide activity and cellular distribution in the brain after intracerebroventricular injectionMolecular Therapy - Nucleic AcidsJune 13, 2023Amy E. Byrnes, Sara L. Dominguez, Chun-Wan Yen, Benjamin I. Laufer, Oded Foreman, Mike Reichelt, Han Lin, Meredith Sagolla, Kathy Hötzel, Hai Ngu, et al.Millipore Sigma) 1:500, rabbit anti-EEA1 (PA5-17228, Thermo Fisher Scientific) 1:100, rat anti-LAMP1 (ab25245, Abcam) 1:500, and rabbit anti-GRASP1 (PA5-34893, Thermo Fisher Scientific) 1:500. Cells were washed

(7) Central carbon flux controls growth/damage balance for Streptococcus pyogenesPLoS pathogensJune 1, 2023Merriman JA, Xu W, Caparon MG.with 5% FBS/5% NGS for 30 min and subsequently incubated with rat anti-LAMP1 antibody (Abcam, cat.#ab25245) for 1 hr at room temperature. Following washes in block buffer, sections were incubated with 18nm colloidal

(8) Apicobasal RNA asymmetries regulate cell fate in the early mouse embryoNature CommunicationsMay 30, 2023Azelle Hawdon, Niall D. Geoghegan, Monika Mohenska, Anja Elsenhans, Charles Ferguson, Jose M. Polo, Robert G. Parton, Jennifer Zenker(Aviva OAAB13856), CAMSAP372 at 1:500, Ninein at 1:500 (MilliPore, MABT29), LAMP1 at 1:1000 (Abcam, ab25245), α-tubulin at 1:1000 (Sigma, T6199), acetylated α-tubulin at 1:1000 (Sigma, 6793), Sec61β at 1:500

(9) Obesity impairs cardiolipin-dependent mitophagy and therapeutic intercellular mitochondrial transfer ability of mesenchymal stem cellsCell death & diseaseMay 13, 2023Sagar S, Faizan MI, Chaudhary N, Singh V, Singh P, Gheware A, Sharma K, Azmi I, Singh VP, Kharya G, et al.,Anti-PGC1 alpha,Abcam,ab54481 4,Anti-MIRO1,Abcam,ab83779 5,Anti-LC3B,Abcam,ab48394 6,Anti-TOMM20,Abcam,ab56783 7,Anti-LAMP1,Abcam,ab25245 8,Recombinant Anti-Beclin 1,Abcam,ab207612 9,Anti-VDAC1/Porin,Abcam,ab15895 10,Anti-PGC1 alpha,Abcam,ab106814 11,Anti-PINK1,Abcam,ab75487 12,Anti-COX

(10) Nicotine exacerbates atherosclerosis and plaque instability via NLRP3 inflammasome activation in vascular smooth muscle cellsTheranosticsMay 8, 2023Junqing An, Liu Ouyang, Changjiang Yu, Sean Michael Carr, Tharmarajan Ramprasath, Zhixue Liu, Ping Song, Ming-Hui Zou, Ye DingMethods Materials and Reagents Antibodies to human NLRP3 (ab109314), Cathepsin B(ab92955) and LAMP1 (ab25245) were purchased from Abcam Technology. Antibodies to β-actin (sc-10731), Caspase1 (sc-56036), Caspase1

(11) DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's diseaseNPJ Parkinson's diseaseMarch 25, 2023Masato A, Plotegher N, Terrin F, Sandre M, Faustini G, Thor A, Adams S, Berti G, Cogo S, De Lazzari F, et al.by Prof. Montecucco’s lab, UNIPD), rabbit anti-αSyn MJFR1 (ab138501, Abcam), rat anti-LAMP1 [1D4B] (ab25245, Abcam), rabbit anti-p62 (ab109012, Abcam), mouse anti-Ubiquitin (P4D1 sc-8017, Santa Cruz Biotech.),

(12) Dysfunctional mitochondria accumulate in a skeletal muscle knockout model of Smn1, the causal gene of spinal muscular atrophyCell death & diseaseFebruary 27, 2023Chemello F, Pozzobon M, Tsansizi LI, Varanita T, Quintana-Cabrera R, Bonesso D, Piccoli M, Lanfranchi G, Giacomello M, Scorrano L, et al.(Merck V9264); LC3 (MBL International, PM036); PARKIN (Santa Cruz Biotechnology sc-32383); LAMP1 (Abcam, ab25245); LAMP2 (Abcam, ab203224); eiF2a (Cell Signaling Technology, 5324) and P-eiF2a (Cell Signaling Technology,

(13) A Trem2R47H mouse model without cryptic splicing drives age- and disease-dependent tissue damage and synaptic loss in response to plaquesMolecular NeurodegenerationFebruary 17, 2023Kristine M. Tran, Shimako Kawauchi, Enikö A. Kramár, Narges Rezaie, Heidi Yahan Liang, Jasmine S. Sakr, Angela Gomez-Arboledas, Miguel A. Arreola, Celia da Cunha, Jimmy Phan, et al.Cambridge, MA), S100β (1:200; AB41548; Abcam), lysosome-associated membrane protein 1 (LAMP1; 1:200; AB25245, Abcam), neurofilament light chain (NfL; 1:200; 171 002; Synaptic Systems, Germany), CD74 (1:500; 151,002;

(14) Trem2 H157Y increases soluble TREM2 production and reduces amyloid pathologyMolecular NeurodegenerationJanuary 31, 2023Wenhui Qiao, Yixing Chen, Jun Zhong, Benjamin J. Madden, Cristine M. Charlesworth, Yuka A. Martens, Chia-Chen Liu, Joshua Knight, Tadafumi C. Ikezu, Aishe Kurti, et al.019-19741, 1:1000), Aβ (MOAB2, Abcam, ab126649, 1:1000), CD68 (Bio-Rad, MCA1957,1:500), LAMP1 (Abcam, ab25245, 1:500), APP (Millipore Sigma, MAB348, 1:300), and GFAP (Millipore Sigma, MAB360, 1:500). To detect

(15) Treatment of tubular damage in high-fat-diet-fed obese mice using sodium-glucose co-transporter inhibitorsPloS oneJanuary 1, 2023Saitoh S, Takaki T, Nakajima K, Wo B, Terashima H, Shimo S, Nguyen HB, Thai TQ, Kumamoto K, Kunisawa K, et al.20 (PBS-T) for 2 h at 25°C, then washed three times with PBS-T. Primary antibodies against LAMP-1 (ab25245, dilution 1:50; Abcam, Cambridge, UK) and aquaporin 1 (AB2219, dilution 1:300; Merck Millipore) were

(16) Elevating microglia TREM2 reduces amyloid seeding and suppresses disease-associated microgliaJournal of Experimental MedicineDecember 5, 2022Na Zhao, Wenhui Qiao, Fuyao Li, Yingxue Ren, Jiaying Zheng, Yuka A. Martens, Xusheng Wang, Ling Li, Chia-Chen Liu, Kai Chen, et al.MAB377, clone A60, 1:1,000; Millipore); anti-Aβ (MOAB-2, Cat# ab126649, 1:1,000; Abcam); anti-LAMP1 (Cat# ab25245, 1:500; Abcam); anti-CD68 (Cat# MCA1957, 1:300; VWR); and anti-CD11c (Cat# 14011482, 1:200; Thermo Fisher Scientific

(17) Vti1a/b support distinct aspects of TGN and cis-/medial Golgi organizationScientific reportsDecember 2, 2022van Bommel DM, Toonen RF, Verhage M.(1:200, Novus Biologicals, NBP1-90440), CgB (1:500, Synaptic Systems, 259 103), LAMP1 (1:500, Abcam, ab25245), Golgin97 (1:50, Santa Cruz, sc-59820), KDEL (1:100, Enzo, ADI-SPA-827), gamma-adaptin (1:250, BD,

(18) Lipid droplet turnover at the lysosome inhibits growth of hepatocellular carcinoma in a BNIP3-dependent mannerScience advancesOctober 14, 2022Berardi DE, Bock-Hughes A, Terry AR, Drake LE, Bozek G, Macleod KF.(Bethyl Laboratories, A190-106A; 1:100), anti-PLN2 (Abcam, ab52356; 1:400), and anti-LAMP1 (Abcam, ab25245; 1:200)] in 10% goat serum in 0.5% TBST overnight at 4°C. The next day, wells were washed in 0.5% TBST

(19) The P522R protective variant of PLCG2 promotes the expression of antigen presentation genes by human microglia in an Alzheimer's disease mouse modelAlzheimer's & DementiaOctober 1, 2022Christel Claes, Whitney E. England, Emma P. Danhash, Sepideh Kiani Shabestari, Amit Jairaman, Jean Paul Chadarevian, Jonathan Hasselmann, Andy P. Tsai, Morgan A. Coburn, Jessica Sanchez, et al.(APOE; 1:1000, Thermo Fisher PA5‐27088), anti‐Ku80 (1:200; Abcam ab79220), anti‐LAMP1 (1:200; Abcam ab25245), anti‐GFAP (1:2000, Abcam ab4674), anti‐Iba1 (1:200; Wako 019‐19741), anti‐CD8 (1:250, Invitrogen 14‐0081‐82)

(20) Spatiotemporal organisation of protein processing in the kidneyNature communicationsSeptember 29, 2022Polesel M, Kaminska M, Haenni D, Bugarski M, Schuh C, Jankovic N, Kaech A, Mateos JM, Berquez M, Hall AM.rabbit anti-Rab11 (D4F5) (1:100, Cell Signaling Technology, #5589), rat anti-Lamp1 (1D4B) (1:100, Abcam, ab25245), mouse anti-Lrp2 (1:200, CD7D5) (Novus Biologicals, NB110-96417), rabbit anti-OAT1 (1:500, Alpha Diagnostic

Be the First to Write a Review!

Anti-LAMP1 antibody [1D4B] from Abcam

Abcam

Citations (163)

Reviews

Anti-LAMP1 antibody [1D4B] from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map