Anti-alpha Tubulin antibody - Microtubule Marker ab18251 from Abcam

Product Specs
ItemAnti-alpha Tubulin antibody - Microtubule Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab18251
Antibody TypePrimary
Quantity50 UG
IsotypeIgG
HostRabbit
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
ClonalityPolyclonal
Conjugate/TagUnconjugated
Concentration0.3 mg/ml
Gene NameTUBA4A
ReactivityHuman, Mouse, Rat
Targetalpha Tubulin

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Fidgetin interacting with microtubule end binding protein EB3 affects axonal regrowth in spinal cord injuryNeural regeneration researchDecember 1, 2023Ma C, Wang J, Tu Q, Bo W, Hu Z, Zhuo R, Wu R, Dong Z, Qiang L, Liu Y, et al.minutes or 2 hours. Primary antibodies were as follows: anti-α-tubulin (rabbit, 1:1200, Abcam, Cat# ab18251, RRID: AB_2210057), anti-tyrosinated tubulin (rat, 1:800, Abcam, Cat# ab6160, RRID: AB_305328), anti-acetylated

(2) O‐GlcNAcylation Regulates Centrosome Behavior and Cell Polarity to Reduce Pulmonary Fibrosis and Maintain the Epithelial PhenotypeAdvanced ScienceNovember 14, 2023Fan Yu, Song Yang, Hua Ni, Dai Heng, Xuemei Wu, Mulin Yang, Xinming Zhang, Yuxin Cao, Yandong Pei, Di An, et al.anti‐E‐cadherin (610 182, BD); anti‐PARD6B (sc166405, Santa Cruz Biotechnology, Dallas, TX); anti‐α‐tubulin (ab18251, Abcam); anti‐γ‐tubulin (ab11316, Abcam); anti‐β‐actin (ab8226, Abcam); anti‐GFP (11 814 460 001, Roche,

(3) Phosphorylation controls spatial and temporal activities of motor‐PRC1 complexes to complete mitosisThe EMBO JournalNovember 2, 2023Agata Gluszek‐Kustusz, Benjamin Craske, Thibault Legal, Toni McHugh, Julie PI Welburntubulin (1:1,000 anti‐beta tubulin, mouse, T7816, Sigma OR 1:2,000/1:1,000 anti‐alpha tubulin, rabbit, ab18251, Abcam) and PRC1 (sc‐376983, Santa Cruz, 1:200 mouse; McHugh et al, 2018). Anti‐CENP‐E (Abcam, Ab5093;

(4) Cross-protection and cross-neutralization capacity of ancestral and VOC-matched SARS-CoV-2 adenoviral vector-based vaccinesnpj VaccinesOctober 4, 2023Sabrina E. Vinzón, María V. Lopez, Eduardo G. A. Cafferata, Ariadna S. Soto, Paula M. Berguer, Luciana Vazquez, Leonora Nusblat, Andrea V. Pontoriero, Eduardo M. Belotti, Natalia R. Salvetti, et al.and probed with an anti-spike Ab (40150-T62, Sino Biological Wayne, PA, USA) and anti-α-tubulin Ab (Ab18251; Abcam, Cambridge, UK). Spike expression was detected and quantified by densitometry using the ImageJ

(5) High-sensitive nascent transcript sequencing reveals BRD4-specific control of widespread enhancer and target gene transcriptionNat CommunAugust 17, 2023Annkatrin Bressin, Olga Jasnovidova, Mirjam Arnold, Elisabeth Altendorfer, Filip Trajkovski, Thomas A. Kratz, Joanna E. Handzlik, Denes Hnisz, Andreas Mayer(Cell Signaling Technology, Cat.# C29F4, 1:1000), H3K27ac (Abcam, ab4729, 1:1000), Tubulin (Abcam, ab18251, 1:3000), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LiCOR, Cat.# 926–32210, 1:15,000), IRDye®

(6) NPC1 plays a role in the trafficking of specific cargo to melanosomesJournal of Biological ChemistryAugust 1, 2023Alina Adriana Rus, Ioana V. Militaru, Ioana Popa, Cristian V.A. Munteanu, Livia Elena Sima, Nick Platt, Frances M. Platt, Ștefana M. Petrescuanti-NPC1 (ab108921), mouse anti-MITF (ab80651), rabbit anti-Calnexin (ab22595) and rabbit anti-tubulin (ab18251) antibodies were purchased from Abcam. Mouse anti-HMB-45 (M06340-1) antibody was from Agilent. LysoTracker Green (DND-26

(7) Super-resolution imaging unveils the self-replication of tau aggregates upon seedingCell reportsJuly 25, 2023Dimou E, Katsinelos T, Meisl G, Tuck BJ, Keeling S, Smith AE, Hidari E, Lam JYL, Burke M, Lövestam S, et al.RRID:AB_10013724" Mouse MC1 antibody,Peter Davies Lab,RRID:AB_2314773 Rabbit anti-alpha tubulin,Abcam,"Cat# ab18251, RRID:AB_2210057" Mouse anti-alpha tubulin,DHSB,"Cat# 12G10, RRID:AB_2315509" Rabbit anti-ubiquitin

(8) Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onseteLifeJuly 11, 2023Mansur A, Joseph R, Kim ES, Jean-Beltran PM, Udeshi ND, Pearce C, Jiang H, Iwase R, Milev MP, Almousa HA, et al.Anti-KBTBD5 for KLHL40, 1:100 dilution (sc-99943, Santa Cruz Biotechnology); anti-α-Tubulin,1:500 (ab18251, Abcam); anti-Sar1a,1:100 (ab125871, Abcam); anti-Sec24d, 1:100 (14687, Cell Signaling technology);

(9) Coordination of actin plus-end dynamics by IQGAP1, formin, and capping proteinbioRxivJuly 6, 2023Morgan L. Pimm, Alexandra G. Marcin, Brian K. Haarer, Marcela Alcaide Eligio, Jessica L. Henty-Ridillaanti-IQGAP1 (1:1000; 610612, BD Biosciences, East Rutherford, NJ), polyclonal rabbit α-tubulin (1:2500; ab18251, Abcam Inc, Cambridge, United Kingdom), and appropriate secondary antibodies (1:5000 IR-dye-680 for

(10) Live-Cell SOFI Correlation with SMLM and AFM ImagingACS Bio & Med Chem AuJune 21, 2023Riley B. Hargreaves, Sam Duwé, Ashley M. Rozario, Alison M. Funston, Rico F. Tabor, Peter Dedecker, Donna R. Whelan, Toby D. M. BellSMLM. Single color microtubule imaging used rabbit anti-α-tubulin 1° antibody (1:500 in 5% BSA, Abcam ab18251) and Alexa Fluor 647 goat anti-rabbit IgG 2° antibody (1:200 in 5% BSA, Thermo Fisher A-21245). For

(11) Cortical dynein drives centrosome clustering in cells with centrosome amplificationMolecular Biology of the CellMay 15, 2023Dayna L. Mercadante, William A. Aaron, Sarah D. Olson, Amity L. Manningmorphology, cells were fixed in ice-cold methanol for 10 min at –20°C. Blocking, primary (α-tubulin: Abcam ab18251, Cambridge, UK; centrin: Millipore 04-1624, Burlington, MA) and secondary antibody dilutions were prepared

(12) Functionally distinct promoter classes initiate transcription via different mechanisms reflected in focused versus dispersed initiation patternsThe EMBO JournalMay 15, 2023Leonid Serebreni, Lisa‐Marie Pleyer, Vanja Haberle, Oliver Hendy, Anna Vlasova, Vincent Loubiere, Filip Nemčko, Katharina Bergauer, Elisabeth Roitinger, Karl Mechtler, et al.anti‐FLAG,Sigma‐Aldrich,Cat#F3165 Secondary anti‐mouse HRP,Sigma‐Aldrich,Cat#12‐349 Histone H3,Abcam,Cat#ab1791 Alpha‐tubulin,Abcam,Cat#Ab18251 Secondary anti‐rabbit HRP,Sigma‐Aldrich,Cat#12‐348 Oligonucleotides and other sequence‐based reagents

(13) Oocyte Arrested at Metaphase II Stage were Derived from Human Pluripotent Stem Cells in vitroStem cell reviews and reportsMay 1, 2023Yu X, Wang N, Wang X, Ren H, Zhang Y, Zhang Y, Qiu Y, Wang H, Wang G, Pei X, et al.32,894, Santa Cruz), c-KIT (1:500, ab5505, Abcam), CX37 (1:300, ab83788, Abcam), and α-Tubulin (1:400, ab18251, Abcam) were used. The same batch of differentiated cells was treated with isotype-matched IgG and used

(14) Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studiesFrontiers in PharmacologyApril 24, 2023Khalid Alghamdi, Zeyad Alehaideb, Ashok Kumar, Hamad Al-Eidi, Sahar S. Alghamdi, Rasha Suliman, Rizwan Ali, Feras Almourfi, Saleh M. Alghamdi, Mohamed Boudjelal, et al.MITF. Mouse monoclonal (clone 236–10501, #A11126, Thermo Fisher Scientific) and rabbit polyclonal (#ab18251, Abcam, Cambridge, MA) anti-α tubulin antibodies were loading controls. Infrared fluorescent dye IRDye®

(15) Heat denaturation enables multicolor X10-STED microscopyScientific reportsApril 1, 2023Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO.#T6199 Sigma-Aldrich, #302211 Synaptic Systems, Göttingen, Germany, #302203 Synaptic Systems, and #ab18251 Abcam, Cambridge, UK), using AbberiorStar635P (AS635P) labeled secondary nanobodies (#N1202-Ab635P-S

(16) Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarityNature communicationsMarch 27, 2023He S, Gillies JP, Zang JL, Córdoba-Beldad CM, Yamamoto I, Fujiwara Y, Grantham J, DeSantis ME, Shibuya H.GR3251355-4), KIF11 (Eg5) (1:100 for IF; Sigma; HPA010568, A118067), α-tubulin (1:1000 for IF; Abcam; ab18251, GR3406015-1), SYCP1 (1:1000 for IF; Abcam; ab15090, GR3184119-1), NuMA (1:100 for IF; Abcam; ab36999,

(17) Selectivity of Hydroxamate- and Difluoromethyloxadiazole-Based Inhibitors of Histone Deacetylase 6 In Vitro and in CellsInternational Journal of Molecular SciencesMarch 1, 2023Jakub Ptacek, Ivan Snajdr, Jiri Schimer, Zsofia Kutil, Jana Mikesova, Petra Baranova, Barbora Havlinova, Werner Tueckmantel, Pavel Majer, Alan Kozikowski, et al.5% BSA in TBS at 4 °C overnight. Antibodies used were anti-alpha tubulin (1:4000 dilution, rabbit, #Ab18251, Abcam, Cambridge, UK), anti-acetylated tubulin (1:2500 dilution, mouse, #T7451, Sigma-Aldrich, St.

(18) CAMSAP2 localizes to the Golgi in islet β-cells and facilitates Golgi-ER traffickingiScienceFebruary 17, 2023Ho KH, Jayathilake A, Mahircan Y, Nour A, Osipovich AB, Magnuson MA, Gu G, Kaverina I.used are as follows: anti-CAMSAP2 (1:500, #NBP1-21402, Novus Biologicals), anti-α-tubulin (1:1000, #ab18251, Abcam), anti-GM130 (1:200, #610823, BD Biosciences), IRDye 800 goat anti-rabbit IgG (1:5000, Rockland

(19) Systematic identification and characterization of repressive domains in Drosophila transcription factorsThe EMBO JournalFebruary 1, 2023Loni Klaus, Bernardo P de Almeida, Anna Vlasova, Filip Nemčko, Alexander Schleiffer, Katharina Bergauer, Lorena Hofbauer, Martina Rath, Alexander StarkChemiDoc MP imaging system (Bio‐Rad). For a loading control, blots were probed with a primary anti‐Tubulin antibody (Abcam, ab18251). Immunoprecipitation‐mass spectrometry (IP‐MS) experiments RDs were cloned into the zfh1‐DSCP‐3xFLAG‐Gal4‐dpse‐EBFP2

(20) Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOGNature CommunicationsJanuary 26, 2023Aamir Ali, Chithran Vineethakumari, Cristina Lacasa, Jens LüdersTim Stearns, Stanford University, USA27), α-Tubulin (1:500, SIMGA T6199), α-Tubulin (1:250, Abcam, ab18251). For the cells expressing the GFP-tagged recombinant proteins, anti-GFP antibody immunostaining was

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3/13/2017Antibody Against Alpha Tubulin For Immunofluorescence Adam KramerThis antibody is great to image the microtubule cytoskeleton in cultured mammalian cells

Anti-alpha Tubulin antibody - Microtubule Marker from Abcam

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Anti-alpha Tubulin antibody - Microtubule Marker from Abcam

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