Fig 1: Cytoplasmic PELP1 and ERR? protect cells from Tam-induced autophagy. A, Cells in G1/G0 from cell cycle analysis of propidium iodide stained HMEC-LXSN and HMEC-Cyto cells treated with vehicle control or 1.0 µM Tam for 24 hours. A student’s T-Test was performed to determine statistical significance for changes in G1/G0 cell populations in response to Tam. P-values are given for the indicated comparisons. B, MTT assay of HMEC-hTERT cells treated with 0.5 or 1.0 µM Tam and ethanol control or 25 µM Z-VAD-FMK. A student’s T-Test was performed to determine statistical significance between control and Z-VAD-FMK treated cells in the presence of Tam. “ns” indicates no statistically significant differences were observed. C, Annexin V/7-AAD staining of HMEC-LXSN and HMEC-Cyto cells treated with 1.0 µM Tam for 24 hours. Student’s T-test was performed to determine statistical significance between Tam treated HMEC-LXSN and HMEC-cyto cells; p-values are shown for the indicated comparisons. D, Western blotting for LC3-I and LC3-II from HMEC-LXSN, HMEC-WT-1/WT-2, and HMEC-cyto lysates treated with ethanol control, 1 µM Tam, or 2 µM Tam for 48 hours. E, Western blot for LC3-II of whole cell lysates from HMECs expressing control or ERR? overexpression vector, treated with control or 0.5, 1, or 2 µM Tam for 24 hours. F, Western blot for LC3 from whole cell lysates of HMEC-LXSN and HMEC-cyto cells expressing either shControl or shERR?, treated with ethanol control or 1 µM Tam for 24 hours.