RAT ANTI MOUSE MACROPHAGES/MONOCYTES MCA519G from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemRAT ANTI MOUSE MACROPHAGES/MONOCYTES
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMCA519G
CloneMOMA-2
TargetMACROPHAGES/MONOCYTES
HostRat
IsotypeIgG2b
Quantity0.25 mg
ApplicationsImmunofluorescence,Flow Cytometry,Immunohistology - Frozen
FormatPurified IgG - liquid
ReactivityMouse
ClonalityMonoclonal Antibody
ConcentrationIgG concentration 0.5 mg/ml

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) PRMT5 inhibition induces pro‐inflammatory macrophage polarization and increased hepatic triglyceride levels without affecting atherosclerosis in miceJournal of Cellular and Molecular MedicineApril 1, 2023Yiheng Zhang, Robin A. F. Verwilligen, Miranda Van Eck, Menno Hoekstrain sections containing atherosclerotic lesions, a primary rat anti‐mouse monoclonal MOMA‐2 antibody (MCA519G; Bio‐Rad) was used at a 1:1000 dilution in blocking buffer. A secondary alkaline phosphatase‐conjugated

(2) Scavenging dicarbonyls with 5'-O-pentyl-pyridoxamine increases HDL net cholesterol efflux capacity and attenuates atherosclerosis and insulin resistanceMolecular metabolismJanuary 1, 2023Huang J, Tao H, Yancey PG, Leuthner Z, May-Zhang LS, Jung JY, Zhang Y, Ding L, Amarnath V, Liu D, et al.NB600-609) CCR2 (cat#: NBP2-67700) were purchased from Novus Biologicals. Primary anti-MOMA2 (cat#: MCA519G) was purchased from BioRad. Secondary antibody HRP conjugated-rabbit anti-goat IgG (cat#: AD106P) and

(3) Interleukin-17A influences the vulnerability rather than the size of established atherosclerotic plaques in apolipoprotein E-deficient miceOpen Life SciencesSeptember 8, 2022Bo Wang, Xitan Hou, Yaning Sun, Chao Lei, Sha Yang, Yao Zhu, Yingming Jiang, Li Songimmunohistochemical staining using rat antimouse macrophage Moma-2 (monocyte/macrophage marker-2) (MCA519G, Bio-Rad Laboratories (Shanghai) Co., Ltd), rabbit antimouse alpha-smooth muscle actin (α-SMA) (ab7817;

(4) Deficiency of ITGAM Attenuates Experimental Abdominal Aortic Aneurysm in MiceJournal of the American Heart AssociationApril 6, 2021Min Zhou, Xia Wang, Yiqin Shi, Yong Ding, Xu Li, Tianchen Xie, Zhenyu Shi, Weiguo Fusections or slides were incubated with diluted primary antibodies against CD11b (ab128797, Abcam), MOMA‐2 (MCA519GT, Bio‐Rad), F4/80 (ab111101, Abcam), iNOS (ab15323, Abcam), and CD206 (PA5‐101657, Invitrogen) at 4°C

(5) Hepatocyte ATF3 protects against atherosclerosis by regulating HDL and bile acid metabolismNature metabolismJanuary 1, 2021Xu Y, Li Y, Jadhav K, Pan X, Zhu Y, Hu S, Chen S, Chen L, Tang Y, Wang HH, et al.kit (Cat # PK-6104. Vector Laboratories) 71. Anti-MOMA2 antibody was purchased from Bio-Rad (Cat # MCA519G) and was used at 1:50 dilution. Hepatic VLDL Secretion and Intestinal Fat or Cholesterol Absorption VLDL

(6) Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysmExperimental & molecular medicineSeptember 1, 2020Jeong SJ, Cho MJ, Ko NY, Kim S, Jung IH, Min JK, Lee SH, Park JG, Oh GT.damage antibody (anti-8-OHG) (ab62623; Abcam), and anti-mouse macrophage/monocyte antibody (anti-MOMA2) (MCA519G; Bio-Rad, Hercules, CA) were used as primary antibodies for immunostaining. After incubation with the

(7) HHIPL1, a Gene at the 14q32 Coronary Artery Disease Locus, Positively Regulates Hedgehog Signaling and Promotes AtherosclerosisCirculationAugust 6, 2019Aravani D, Morris GE, Jones PD, Tattersall HK, Karamanavi E, Kaiser MA, Kostogrys RB, Ghaderi Najafabadi M, Andrews SL, Nath M, et al.smooth muscle α-actin 2 (ACTA2), and macrophages were stained using macrophages/monocytes antibody (MOMA-2; MCA519G, Bio-Rad). Slides were fixed in cold acetone at −20°C and air dried, and endogenous peroxidase activity

(8) Myeloid Slc2a1-Deficient Murine Model Revealed Macrophage Activation and Metabolic Phenotype Are Fueled by GLUT1Journal of immunology (Baltimore, Md. : 1950)February 15, 2019Freemerman AJ, Zhao L, Pingili AK, Teng B, Cozzo AJ, Fuller AM, Johnson AR, Milner JJ, Lim MF, Galanko JA, et al.NY). Abs were purchased from the following sources: anti-F4/80 (MCA497) and MF/monocyte mAb (MOMA2) (MCA519G) (AbD Serotec/Bio-Rad, Hercules, CA), anti-GLUT1 (ab40084; Abcam, Cambridge, MA), anti-actin (MAB1501)

(9) Myeloid HMG-CoA (3-Hydroxy-3-Methylglutaryl-Coenzyme A) Reductase Determines Atherosclerosis by Modulating Migration of MacrophagesArteriosclerosis, Thrombosis, and Vascular BiologyNovember 1, 2018Kent Sakai et al.were incubated with a primary anti-MOMA2 (monocyte+macrophage) antibody (0.8 µg/mL; Bio-Rad, Cat No MCA519G;) overnight at 4°C in a buffer. After washing, the sections were incubated with biotinylated secondary

(10) IRF3 and type I interferons fuel a fatal response to myocardial infarctionNature MedicineDecember 1, 2017Kevin R. King et al.neutrophils, monocytes, and macrophages (CD11b, M1/70, BD Biosciences); monocytes and macrophages (MOMA-2, MCA519G, AbD Serotec); and collagen deposition (Collagen I, ab32386, Abcam). Biotinylated anti-rat IgG (for

(11) Lack of myeloid Fatp1 increases atherosclerotic lesion size in Ldlr −/− miceAtherosclerosisNovember 1, 2017Liyang Zhao, Alyssa J. Cozzo, Amy R. Johnson, Taylor Christensen, Alex J. Freemermana, James E. Bear, Jeremy D. Rotty, Brian J. Bennett, Liza Makowskianti-IL6 (interleukin 6) for inflammation. The antibodies used for immunostaining were: MOMA2 (BioRad, MCA519G), anti α-SMA (Sigma, A5228), anti 4HNE (Abcam, ab48506), and anti IL6 (Novusbio, NB600-1131), which

(12) Systemic application of 3-methyladenine markedly inhibited atherosclerotic lesion in ApoE^-/- mice by modulating autophagy, foam cell formation and immune-negative moleculesCell death & diseaseDecember 1, 2016Dai S, Wang B, Li W, Wang L, Song X, Guo C, Li Y, Liu F, Zhu F, Wang Q, et al.underwent immunohistochemistry (IHC) staining with a rat anti-mouse macrophage-specific antibody (MOMA-2; MCA519G, AbD Serotec, Oxford, UK) or a rabbit anti-mouse polyclonal antibody for smooth muscle actin (ab5694,

(13) Serum Amyloid A Facilitates Early Lesion Development in Ldlr-/- MiceJournal of the American Heart AssociationJuly 17, 2015Krishack PA, Bhanvadia CV, Lukens J, Sontag TJ, De Beer MC, Getz GS, Reardon CA.Goat anti-rabbit antibody (A-4914) was purchased from Sigma-Aldrich (A-4914). Rat anti-mouse MOMA-2 (MCA519G) was purchase from AbD Serotec. Rabbit anti-rat IgG H+L chain AF594 was purchased from Invitrogen. Primers

(14) PDGFRβ signalling regulates local inflammation and synergizes with hypercholesterolaemia to promote atherosclerosisNature communicationsJuly 17, 2015He C, Medley SC, Hu T, Hinsdale ME, Lupu F, Virmani R, Olson LE.antibodies recognizing PECAM (BD Bioscience 553370, 1:100), αSMA (Sigma F3777, 1:250), MOMA-2 (AbD Serotec MCA519G, 1:100), F4/80 (Abcam ab6640, 1:100), SMMHC (Biomedical Technologies Inc. BT-562, 1:100) or Sm22α (Abcam

(15) Molecular hydrogen stabilizes atherosclerotic plaque in low-density lipoprotein receptor-knockout miceFree Radical Biology and MedicineJune 4, 2015Guohua Song, Chuanlong Zong, Zhaoqiang Zhang, Yang Yu, Shutong Yao, Peng Jiao, Hua Tian, Lei Zhai, Hui Zhao, Shuyan Tian, Xiangjian Zhang, Yun Wu, Xuejun Sun, Shucun Qin

(16) Tumor necrosis factor-like weak inducer of apoptosis or Fn14 deficiency reduce elastase perfusion-induced aortic abdominal aneurysm in miceJournal of the American Heart AssociationAugust 4, 2014Tarín C, Fernández-Laso V, Sastre C, Madrigal-Matute J, Gómez M, Zaragoza C, Egido J, Burkly LC, Martín-Ventura JL, Blanco-Colio LM.carried out as previously described.16 Primary antibodies were the monocyte/macrophage marker MOMA‐2 (MCA519G; AbD Serotec), the smooth muscle cell marker smooth muscle actin (Clone 1A4; Sigma), the neutrophil

(17) The adipokine Retnla modulates cholesterol homeostasis in hyperlipidemic miceNature communicationsJuly 15, 2014Lee MR, Lim CJ, Lee YH, Park JG, Sonn SK, Lee MN, Jung IH, Jeong SJ, Jeon S, Lee M, et al.International (cat. No. K23300R, rabbit IgG), the antibodies to Moma-2 were obtained from AbD Serotec (cat. No. MCA519G, rat IgG) and GAPDH antibody was from Santa Cruz Biotechnology (cat No. SC-25778, rabbit IgG). Immunoﬂuorescence

(18) Simvastatin and Losartan Differentially and Synergistically Inhibit Atherosclerosis in Apolipoprotein E^-/-MiceKorean Circulation JournalAugust 31, 2012Bok-Soo Lee, Jin Yong Choi, Joo Yun Kim, Seul Hee Han, Jeong Euy Parkdilutions) overnight at 4°C. Primary antibodies used in the study were as follows: anti-MOMA2 (Cat #MCA519GT, AbD Serotec), anti-SMC actin (Cat#ab7817, Abcam), anti-metalloproteinase 9 (MMP9) (Cat#ab38898, Abcam),

(19) Autophagy links inflammasomes to atherosclerotic progressionCell metabolismApril 4, 2012Razani B, Feng C, Coleman T, Emanuel R, Wen H, Hwang S, Ting JP, Virgin HW, Kastan MB, Semenkovich CF.BmbH, Heidelberg, Germany; LC3/ATG8 (#2775) from Cell Signaling Technology Inc., Danvers, MA; MOMA-2 (MCA519G) from AbD Serotec, Oxford, UK; α-smooth muscle actin (1A4 – FITC conjugate) from Sigma-Aldrich Corp.,

(20) Differential effects on inhibition of cholesterol absorption by plant stanol and plant sterol esters in apoE-/- miceCardiovascular ResearchJune 1, 2011Oliver Weingartner, Christof Ulrich, Dieter Lutjohann, Kenan Ismail, Stephan H. Schirmer, Tim Vanmierlo, Michael Bohm, Ulrich Laufs

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RAT ANTI MOUSE MACROPHAGES/MONOCYTES

RAT ANTI MOUSE MACROPHAGES/MONOCYTES

RAT ANTI MOUSE MACROPHAGES/MONOCYTES

RAT ANTI MOUSE MACROPHAGES/MONOCYTES from Bio-Rad (Formerly AbD Serotec)

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RAT ANTI MOUSE MACROPHAGES/MONOCYTES from Bio-Rad (Formerly AbD Serotec)

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