Fig 2: Phagocytosis of Tumor Cells by IPSDMs and PBDMs(A) FACS analysis of CD47 on Jurkat cells. Secondary antibody only was used as a negative control.(B) FACS analysis of CD172a on M0-IPSDMs and M0-PBDMs. Non-stained cells were used as negative control.(C) A representative image of Jurkat cells (labeled with green fluorescent dye) phagocytized by M0-IPSDMs (phase contrast image). Scale bar represents 50 µm. CFSE-labeled Jurkat cells were incubated with anti-CD47 blocking antibody and co-cultured with M0-IPSDMs for 30 min.(D) FACS analysis of Jurkat cell phagocytosis by M0-IPSDMs and M0-PBDMs. CFSE-labeled Jurkat cells were incubated with or without anti-CD47 blocking antibody and added to macrophages for 2 h. CD11b+ macrophages are gated (upper panel), and their CFSE intensity is shown as a histogram (lower panel).(E) Phagocytotic index of M0-IPSDMs and M0-PBDMs with and without CD47 blocking antibody. The percentage of CFSE+ macrophages was multiplied by the MFI of CFSE to get the phagocytotic index. Error bars are ±SD of four independent experiments. Uncorrected Fisher's least significant differences test: ns, non-significant; ***p < 0.001.M0-IPSDMs were differentiated from LU83 in (B)–(E). See also Figure S5 and Video S3.