Alexa Fluor(R) 488 anti-mouse CD4 100423 from BioLegend

Product Specs
ItemAlexa Fluor(R) 488 anti-mouse CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100423
NCBI Gene AliasesL3T4, Ly-4
ReactivityMouse
IsotypeIgG2b, Kappa
CloneGK1.5
Quantity100 µg
Conjugate/TagAlexa Fluor® 488
ApplicationsFlow Cytometry (FC), Immunohistochemistry (IHC)-F, Immunocytochemistry (ICC)
HostRat
Antibody TypePrimary
NCBI Full Gene NameCD4 antigen
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenMouse CTL clone V4
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Local and Systemic Injections of Human Cord Blood Myeloid-Derived Suppressor Cells to Prevent Graft Rejection in Corneal TransplantationBiomedicinesDecember 12, 2022Lee JY, Sohn HJ, Kim CH, Kim TG, Lee HS.115010, BioLegend, San Diego, CA, USA), Alexa Fluor 488-anti-mouse CD4 antibody (1:100, host:mouse, cat# 100423, BioLegend, San Diego, CA, USA), and PE-anti-mouse IFN-gamma antibody (1:100, host:rat, cat# 12-7311-82,

(2) Forced expression of the non-coding RNA miR-17âˆ¼92 restores activation and function in CD28-deficient CD4+ T cellsiScienceNovember 18, 2022Marianne DÃ¶lz, Marko Hasiuk, John D. Gagnon, Mara Kornete, Romina Marone, Glenn Bantug, Robin Kageyama, Christoph Hess, K. Mark Ansel, Denis Seyres, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Rat anti-mouse CD4 clone GK1.5 or RM4-5,Biolegend,"Cat#100423, 100532" Rat anti-mouse/human CD44 clone IM7,Biolegend,"Cat#103032, 103008" Rat anti-mouse CD62L clone

(3) Local and systemic inflammation triggers different outcomes of tumor growth related to infiltration of anti-tumor or pro-tumor macrophagesChinese medical journalAugust 5, 2022Liu X, Jiang Q, Shen S, Hou Y.eBioscience), CD3-APC (17-0031-81, eBioscience), CD8-FITC (11-0081-81, eBioscience), CD4-Alexa Fluor 488 (100423, BioLegend). Stained cells were detected by a fluorescence-activated cell sorting Calibur flow cytometer

(4) An acquired phosphatidylinositol 4-phosphate transport initiates T-cell deterioration and leukemogenesisNature communicationsJuly 29, 2022Zhong W, Lin W, Yang Y, Chen D, Cao X, Xu M, Pan G, Chen H, Zheng J, Feng X, et al.PE anti-mouse CD8a (Biolegend, Cat# 100708, clone: 53-6.7, 1:100), Alexa Fluor-488 anti-mouse CD4 (Biolegend, Cat# 100423, clone: GK1.5, 1:100), Alexa Fluor-647 anti-mouse c-Kit (Biolegend, Cat# 105818, clone: 2B8, 1:100),

(5) Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cellsJournal of translational medicineFebruary 11, 2022Park JS, Lee D, Yang S, Jeong HY, Na HS, Cho KH, Choi J, Koo H, Cho ML, Park SH.following antibodies were used for confocal microscopy: rat monoclonal Alexa Fluor® 488 anti-CD4 (GK1.5, #100423) and rat monoclonal APC anti-CD25 (PC6, #102012) antibodies were from BioLegend; rat monoclonal PE anti-IL-17A

(6) Comparative transcriptomic analysis reveals translationally relevant processes in mouse models of malariaeLifeJanuary 10, 2022Athina Georgiadou, Claire Dunican, Pablo Soro-Barrio, Hyun Jae Lee, Myrsini Kaforou, Aubrey J CunningtonRRID:AB_11204249,(2:100) Antibody,Alexa Fluor 488 anti-mouse CD4 Clone GK1.5 Rat monoclonal antibody,BioLegend,(100425) RRID:AB_493520,(1:200) Antibody,APC anti-mouse CD4 Clone GK1.5 Rat monoclonal antibody,BioLegend,(100411)

(7) RGS10 Reduces Lethal Influenza Infection and Associated Lung Inflammation in MiceFrontiers in ImmunologyNovember 29, 2021Faris Almutairi, Demba Sarr, Samantha L. Tucker, Kayla Fantone, Jae-Kyung Lee, Balázs Rada‘lymphoid antibody cocktail’ [CD45-APC/F750 (Cat#: 103154), CD3-APC (Cat#: 100236), CD4-AF488 (Cat#: 100423), CD8a-AF700 (Cat#: 100766), B220-BV785 (Cat#: 103246), and NK 1.1-PE (Cat#: 108708)], all from Biolegend

(8) Expansion of CD10neg neutrophils and CD14+HLA-DRneg/low monocytes driving proinflammatory responses in patients with acute myocardial infarctioneLifeJuly 22, 2021Daniela Fraccarollo, Jonas Neuser, Julian Möller, Christian Riehle, Paolo Galuppo, Johann Bauersachs550993 RRID:AB_394002,1:100 antibody,anti-mouse CD4-Alexa Fluor 488 (Rat monoclonal),BioLegend,Cat# 100423 RRID:AB_389302,1:100 antibody,anti-mouse CD115-Brilliant Violet 605 (Rat monoclonal),BioLegend,Cat#

(9) CD8⁺ T cells inhibit metastasis and CXCL4 regulates its functionBritish journal of cancerJuly 1, 2021Joseph R, Soundararajan R, Vasaikar S, Yang F, Allton KL, Tian L, den Hollander P, Isgandarova S, Haemmerle M, Mino B, et al.

(10) Influenza virus infection expands the breadth of antibody responses through IL-4 signalling in B cellsNature communicationsJune 18, 2021Miyauchi K, Adachi Y, Tonouchi K, Yajima T, Harada Y, Fukuyama H, Deno S, Iwakura Y, Yoshimura A, Hasegawa H, et al.(clone: 145-2C11; 1:200 dilution, Biolegend, 100304), CD4 (clone: GK1.5; 1:1000 dilution, Biolegend, 100423), CD5 (clone: 53-7.3; 1:200 dilution, Biolegend, 100604), CD8a (clone: 53-6.7; 1:200 dilution, Biolegend,

(11) Disrupting tumour vasculature and recruitment of aPDL1-loaded platelets control tumour metastasisNature communicationsMay 13, 2021Li H, Wang Z, Chen Z, Ci T, Chen G, Wen D, Li R, Wang J, Meng H, Bryan Bell R, et al.(Biolegend, cat no. 100708, clone: 53-6.7) and Alexa Fluor® 488 anti-mouse CD4 antibody (Biolegend, cat no. 100425, clone: GK1.5) was used to stain the tumour slices overnight at 4 °C. The tumour cell apoptosis was

(12) Obesity results in adipose tissue T cell exhaustionJCI insightApril 22, 2021Porsche CE, Delproposto JB, Geletka L, O'Rourke R, Lumeng CN.antibodies for 30 minutes at 4°C. Anti-mouse antibodies used included the following: AF488-CD4 (catalog 100423), APCcy7-CD8 (catalog 100713), Brilliant Violet 605-CD279 (PD1) (catalog 135219), PE/Cy7-CD28 (catalog

(13) Fc-optimized antibodies elicit CD8 immunity to viral respiratory infectionNatureDecember 17, 2020Stylianos Bournazos, Davide Corti, Herbert W. Virgin, Jeffrey V. Ravetch

(14) SARS-CoV-2 RBD Neutralizing Antibody Induction is Enhanced by Particulate VaccinationAdvanced materials (Deerfield Beach, Fla.)December 1, 2020Huang WC, Zhou S, He X, Chiem K, Mabrouk MT, Nissly RH, Bird IM, Strauss M, Sambhara S, Ortega J, et al.B243491), PD‐1 PE (Clone: 29F.1A12; Cat # 135205; Lot: B251877), Alexa Fluor 488 CD4 (Clone: GK1.5; Cat # 100425; Lot: B238433). For intracellular cytokine staining: Surface markers to identified CD4+ and CD8+ T cells

(15) Cancer Cell Coating Nanoparticles for Optimal Tumor-Specific Cytokine DeliveryACS nanoSeptember 22, 2020Barberio AE, Smith SG, Correa S, Nguyen C, Nhan B, Melo M, Tokatlian T, Suh H, Irvine DJ, Hammond PT.

(16) Microbial therapeutics for acute colitis based on genetically modified Lactococcus lactis hypersecreting IL-1Ra in miceExperimental & molecular medicineSeptember 1, 2020Namai F, Shigemori S, Ogita T, Sato T, Shimosato T.TNF-α, IL-1β, IL-6, and IL-17) were obtained from TaKaRa Bio, Inc. Alexa Fluor® 488 anti-mouse CD4 (CAT# 100423; BioLegend) and PerCP/Cy5.5 anti-mouse IL-17A antibodies (CAT# 506919; BioLegend) were used. MLN cells

(17) Mechanosensing through YAP controls T cell activation and metabolismThe Journal of experimental medicineAugust 3, 2020Meng KP, Majedi FS, Thauland TJ, Butte MJ.following BioLegend antibodies were used for surface staining: CD4 (clones RM4-5 and GK1.5, 100532 and 100425); CD8 (clone 53–6.7, 100724); B220 (clone RA3-6B2, 103244); CD45.1 (clone A20, 110732, 110715); CD45.2

(18) CD4+ T cell expression of the IL-10 receptor is necessary for facial motoneuron survival after axotomyJournal of neuroinflammationApril 17, 2020Runge EM, Iyer AK, Setter DO, Kennedy FM, Sanders VM, Jones KJ.no.,Dilution Primary,Rabbit anti-IBA1,Thermo Fisher 019-19741,1:500 Primary,Rat anti-CD4 488,BioLegend 100423,1:100 Primary,Rabbit anti-CD3,Abcam ab16669,1:200 Primary,Rabbit anti-CD31,Abcam ab124432,1:500 Secondary,"Donkey

(19) Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunityNature communicationsMarch 20, 2020von Roemeling CA, Wang Y, Qie Y, Yuan H, Zhao H, Liu X, Yang Z, Yang M, Deng W, Bruno KA, et al.0.25 µg/106 cells), CD3 (clone 17A2; BioLegend, #100201, 0.25 µg/106 cells), CD4 (GK1.5; BioLegend, #100425, 0.25 µg/106 cells), CD8 (53-6.7; BioLegend, #100727, 0.25 µg/106 cells), FoxP3 (clone MF-14; BioLegend,

(20) Systematic Evaluation of Chemically Distinct Tissue Optical Clearing Techniques in Murine Lymph NodesJournal of immunology (Baltimore, Md. : 1950)March 1, 2020Matryba P, Sosnowska A, Wolny A, Bozycki L, Greig A, Grzybowski J, Stefaniuk M, Nowis D, Gołąb J.100240; BioLegend), anti-mouse CD4 conjugated with Alexa Fluor 488 (1:200 dilution; clone GK1.5, no. 100423; BioLegend), anti-mouse CD8 conjugated with Alexa Fluor 647 (1:200 dilution; clone 53–6.7, no. 100724;

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(1)

12/22/2023Anti-Mouse CD4 Antibody From BioLegend Shows Great Staining Jordan ZhouThis antibody gives great CD4 staining in mouse intestine cells. The positive signal is strong and specific.
(1)

3/15/2017Good Antibody To Stain For Mouse CD4+ T Cells Kevin MengSuccessfully detects CD4+ T cells in thymus, spleen, and lymph node with high signal.
(1)

6/21/2016Great Antibody Andy GonzalezI used this antibody in my flow cytometry panel in hopes of defining my CD4 T cell population. This antibody is a little nerve-racking because it recommends such a small amount to use, but it does ... Read Review
(1)

11/17/2015Detection of CD4 Positive T Cells in Mouse Breast Cancer Tumors Dinesh AhirwarThe orthotopic syngeneic mouse tumors established using MVT1 cell line were analyzed for the presence of CD4+/CD3+ T cells using Alexa Fluor® 488 anti-mouse CD4 Antibody. The tumors were pulverized ... Read Review

Alexa Fluor(R) 488 anti-mouse CD4 from BioLegend

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Alexa Fluor(R) 488 anti-mouse CD4 from BioLegend

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