GFP Antibody NB600-308 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemGFP Antibody
CompanyNovus Biologicals, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberNB600-308
ImmunogenThe immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
HostRabbit
Quantity0.1 mg (also 0.025 mg)
Format0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
ReactivityNon-species specific
ApplicationsWestern Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Dot Blot, Electron Microscopy, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount, Knockdown Validated
Conjugate/TagUnconjugated
TargetGFP
IsotypeIgG
Brand/TrademarkNovus Biologicals, a Bio-Techne Brand
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaEpitope Tags
Antibody TypePrimary Antibodies
StorageStore at -20C. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Unconjugated

Specificity: No reaction was observed against Human, Mouse or Rat serum proteins

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: http://www.novusbio.com

(1) C-Terminal Domain Controls Protein Quality and Secretion of Spider Silk in Tobacco CellsAdv Biol (Weinh)December 1, 2023Thagun C, Suzuki T, Kodama Y, Numata K.

(2) The SYP123-VAMP727 SNARE complex delivers secondary cell wall components for root hair shank hardening in ArabidopsisThe Plant cellNovember 30, 2023Hirano T, Ebine K, Ueda T, Higaki T, Watanabe-Nakayama T, Konno H, Takigawa-Imamura H, Sato MH.

(3) A brainstem circuit for the expression of defensive facial reactions in ratCurrent biology : CBSeptember 25, 2023Callado Pérez A, Demers M, Fassihi A, Moore JD, Kleinfeld D, Deschênes M.

(4) Dopamine release in the nucleus accumbens promotes REM sleep and cataplexyiScienceSeptember 15, 2023Toth BA, Chang KS, Fechtali S, Burgess CR.

(5) The energetics and ion coupling of cholesterol transport through Patched1Science advancesAugust 25, 2023Ansell TB, Corey RA, Viti LV, Kinnebrew M, Rohatgi R, Siebold C, Sansom MSP.hPTCH1-mVenus-1D4 gene. An anti–green fluorescent protein antibody (Novus Biologicals, catalog no. NB600-308, RRID: AB_10003058) was used to detect PTCH1 expression levels, and anti-P38 (Abcam, catalog no. ab7952,

(6) Autism-linked UBE3A gain-of-function mutation causes interneuron and behavioral phenotypes when inherited maternally or paternally in miceCell reportsJuly 25, 2023Xing L, Simon JM, Ptacek TS, Yi JJ, Loo L, Mao H, Wolter JM, McCoy ES, Paranjape SR, Taylor-Blake B, et al.(Sigma-Aldrich, A1978; 1:5,000), mouse anti-Myc tag (Millipore, 05–724; 1:3,000), rabbit anti-GFP (Novus, NB600–308; 1:2,000). Western blot signals of proteins of interest were measured in ImageJ and normalized to β-ACTIN

(7) The EGF-motif-containing protein SPE-36 is a secreted sperm protein required for fertilization in C. elegansCurrent biology : CBJuly 24, 2023Krauchunas AR, Marcello MR, Looper A, Mei X, Putiri E, Singaravelu G, Ahmed II, Singson A.

(8) Quantitative intravital imaging for real-time monitoring of pancreatic tumor cell hypoxia and stroma in an orthotopic mouse modelScience advancesJune 9, 2023Samuel T, Rapic S, O'Brien C, Edson M, Zhong Y, DaCosta RS.no.",Dilution,Secondary antibody/reagent,"Company, catalog no.",Dilution Western blot,Anti-GFP,"Novus Biologicals, NB600-308",1:2000,IRDye 680RD Detection Reagent,"LI-COR Biosciences, 926-69100",1 μl/ml Western blot,IRDye 680RD

(9) A bifurcated role for c-Maf in Th2 and Tfh2 cells during helminth infectionMucosal immunologyJune 1, 2023Bao K, Isik Can U, Miller MM, Brown IK, Dell'Aringa M, Dooms H, Seibold MA, Scott-Browne J, Lee Reinhardt R.endogenous biotin (Avidin/Biotin Block; Vector Labs, Newark, CA, USA). eGFP was detected by anti-mouse-GFP (NB600–308; Novus Biologicals, Centennial, CO, USA), followed by biotinylated F(ab’)2 donkey anti-rabbit (1:500;

(10) Striatal dopamine regulates sleep states and narcolepsy-cataplexybioRxiv : the preprint server for biologyMay 30, 2023Toth BA, Chang KS, Burgess CR.temperature in blocking solution containing primary antiserum (rabbit anti-GFP, 488-conjugated, Novus #NB600–308, 1:5000; rabbit anti-TH, Thermo Fisher Scientific #OPA1–04050, 1:5000). The next morning, sections were

(11) Liver-directed gene therapy for ornithine aminotransferase deficiencyEMBO molecular medicineApril 11, 2023Boffa I, Polishchuk E, De Stefano L, Dell'Aquila F, Nusco E, Marrocco E, Audano M, Pedretti S, Caterino M, Bellezza I, et al.

(12) Endothelial cells overexpressing CXCR1/2 are renoprotective in rats with acute kidney injuryAmerican journal of physiology. Renal physiologyApril 1, 2023Xing D, Hage FG, Feng W, Guo Y, Oparil S, Sanders PW.

(13) Atg1 modulates mitochondrial dynamics to promote germline stem cell maintenance in DrosophilaBiochemical and biophysical research communicationsFebruary 5, 2023Ayachit MS, Shravage BV.

(14) Differential requirement for BRCA1-BARD1 E3 ubiquitin ligase activity in DNA damage repair and meiosis in the Caenorhabditis elegans germ linePLoS geneticsJanuary 1, 2023Li Q, Kaur A, Okada K, McKenney RJ, Engebrecht J.and probed with mouse anti-FLAG (MA1-91878; Invitrogen; 1:1000; RRID AB_1957945), rabbit anti-GFP (NB600-308; Novus Biologicals; 1:2000; RRID: AB_10003058), mouse anti-HA [12CA5; amino acids 98–106 of human influenza

(15) Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hairBioactive materialsJanuary 1, 2023Ma L, Chen C, Liu D, Huang Z, Li J, Liu H, Kin Kwok RT, Tang B, Sui B, Zhang X, et al.Anti-mouse active-β-catenin (05665) was purchased from EMD Millipore (Billerica, MA, USA). Anti-rabbit GFP (NB600-308) was purchased from Novus Biologicals (Centennial, CO, USA). Anti-rabbit DKK1 (AF4600) was purchased

(16) Direct implantation of hair-follicle-associated pluripotent (HAP) stem cells repairs intracerebral hemorrhage and reduces neuroinflammation in mouse modelPloS oneJanuary 1, 2023Obara K, Shirai K, Hamada Y, Arakawa N, Hasegawa A, Takaoka N, Aki R, Hoffman RM, Amoh Y.

(17) Transplanted human induced pluripotent stem cells- derived retinal ganglion cells embed within mouse retinas and are electrophysiologically functionaliScienceNovember 18, 2022Vrathasha V, Nikonov S, Bell BA, He J, Bungatavula Y, Uyhazi KE, Murthy Chavali VR.Tubulin β 3 (TUJ1; BioLegend, catalog #: 801201, San Diego, CA, USA), GFP (Novus Biologicals, catalog #: NB600-308, Littleton, CO, USA), PSD95 (Cell Signaling Technology, catalog #: 3409S, Danvers, MA, USA), VGLUT2

(18) SMAD4 Suppresses Colitis-associated Carcinoma Through Inhibition of CCL20/CCR6-mediated InflammationGastroenterologyNovember 1, 2022Hanna DN, Smith PM, Novitskiy SV, Washington MK, Zi J, Weaver CJ, Hamaamen JA, Lewis KB, Zhu J, Yang J, et al.

(19) A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migrationCell reportsOctober 18, 2022Ghochani Y, Muthukrishnan SD, Sohrabi A, Kawaguchi R, Condro MC, Bastola S, Gao F, Qin Y, Mottahedeh J, Iruela-Arispe ML, et al.REA730",Miltenyi Biotec,Cat#: 130-110-670; RRID:AB_2657283 Rabbit polyclonal anti-GFP,Novus Biologicals,Cat#: NB600-308; RRID:AB_341929 Mouse monoclonal anti-mCherry,Novus Biologicals,Cat#: NBP1-96752; RRID:AB_11034849 Chicken

(20) Inka2, a novel Pak4 inhibitor, regulates actin dynamics in neuronal developmentPLoS geneticsOctober 1, 2022Yamada S, Mizukoshi T, Tokunaga A, Sakakibara SI.(rabbit polyclonal, GTX113617, GeneTex, Irvine, CA, USA; 1:2000 for WB), anti-GFP (rabbit polyclonal, NB600-308, Novus Biologicals, Littleton, CO, USA; 1:500 for IP), anti-DDDDK (Flag) (mouse monoclonal, FLA-1, MBL

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(1)

7/31/2023Great GFP Antibody for Western Blot and Immunofluorescence From Novus Biologicals Parijat SarkarI use the GFP antibody to detect the expression level of GFP-tagged proteins in my experiments. By using the GFP tag, I can easily visualize and quantify the presence of the tagged protein on a ... Read Review
(1)

11/18/2019Good GFP Antibody from Novus Abhijit NirwaneGood antibody to detect endogenous GFP.
(1)

9/15/2016GFP Antibody For Immunofluorescence Staining Ganesh PusapatiG-protein coupled receptor kinase 2 (GRK2) phosphorylates and desensitizes activated GPCRs on the plasma membrane to regulate the GPCR on/off cycle in signal transduction events. GRK2 displays a ... Read Review
(1)

1/13/2016GFP Antibody for Western Blotting Ganesh PusapatiThe goal of this experiment was to test the GFP antibody from Novus Biologicals for Western Blotting. I wanted to test if this antibody works at higher dilution like 1:5000-1:10000
(1)

5/20/2015Immunoprecipitation of GFP-fusion protein Marcio FonteneleThis antibody was used in a study that investigated the role of Capain-A protease in the Dorsal/Cactus (NFkB/IkB) activities during Drosophila embryogenesis.

GFP Antibody

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GFP Antibody from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Novus Biologicals, a Bio-Techne Brand

Citations (251)

Reviews

GFP Antibody from Novus Biologicals, a Bio-Techne Brand

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