RAT ANTI HUMAN HLA DR

Published customer image:
Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometry
Image caption:
BILF1 identified as a lytic gene that downregulates surface MHC class I. 293 (A) or MJS (B) cells were transfected with different EBV genes in the bi-cistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I was stained with PE-conjugated W6/32 mAb and (in MJS only) MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyse staining in the untransfected GFP− population, shown as the solid line histogram, and in the transfected GFP+ population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody.

From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009)
The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation.
.
This image is from an open access article distributed under terms of a.

Published customer image:
Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometry
Image caption:
BILF1 identified as a lytic gene that downregulates surface MHC class I. 293 (A) or MJS (B) cells were transfected with different EBV genes in the bi-cistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I was stained with PE-conjugated W6/32 mAb and (in MJS only) MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyse staining in the untransfected GFP− population, shown as the solid line histogram, and in the transfected GFP+ population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody.

From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009)
The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation.
PLoS Pathog 5(1): e1000255.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.