Fig 1: (A–C) Representative images of CTCs isolated from NSCLC patients stained by antibodies against cytokeratin (red), a leukocyte marker CD45 (green), and a nuclear stain (DAPI). (A) CK (+)/PD-L1 (+) CTC, (B) CK (+)/PD-L1 (-) CTC, (C) CTC cluster with WBC. Scale bar is 10 µm. (D) CTC enumeration from blood samples of healthy donors (n = 6) and from blood samples of NSCLC patients (n = 38), (***)P-value < 0.001. (E) Number of CTCs isolated by GO chip from blood samples from different visits of 13 NSCLC patients (P1 through P13). ‘P1’ stands for patient 1. Blue bar represents the number of CK (+)/PD-L1 (-) CTCs. Red bar represents the number of CK (+)/PD-L1 (+) CTCs.