Angiopoietin 1 Antibody 100-401-403 from Rockland Immunochemicals, Inc.

Product Specs
ItemAngiopoietin 1 Antibody
CompanyRockland Immunochemicals, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100-401-403
Quantity200 µL
Conjugate/TagUnconjugated
HostRabbit
ImmunogenThis whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling
ApplicationsELISA,Immunohistochemistry,Immunoprecipitation,Western Blot
Concentration80 mg/mL
ClonalityPolyclonal
ReactivityHuman, Mouse, Mouse
PurityThis antiserum is directed against mouse angiopoietin 1 and shows no reactivity with mouse angiopoietin 2. A 57 kDa band is detected when assayed by immunoblot. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2
Domain/Region/TerminusN-Terminus
NCBI Gene Aliases1110046O21Rik, Ang, Ang1, Ang-1, an
Antibody TypePrimary
FormatLiquid (sterile filtered)
TargetAngiopoietin 1
Gene NameAngpt1
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Note: Anti-Antiopoietin-1 Antiserum has been tested in ELISA, western blot, immunohistochemistry, and immunofluorescence and is suitable for other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.

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Rockland Immunochemicals, Inc.

P.O. Box 5199

Limerick, Pennsylvania 19468

United States

Phone: (484) 791-3823
Fax: (484) 369-8654

Company Profile

Website: https://www.rockland.com

(1) Shear stress-induced angiogenesis in mouse muscle is independent of the vasodilator mechanism and quickly reversibleActa physiologica (Oxford, England)November 1, 2016Egginton S, Hussain A, Hall-Jones J, Chaudhry B, Syeda F, Glen KE.Santa Cruz Biotechnology, cat#SC‐504), Ang‐1 (1 : 500, Rockland Immunochemicals, Limerick, PA, USA, cat#100‐401‐403), Tie‐2 (1 : 1000; R&D Systems, Abingdon, UK, cat#AF762), TSP‐1 (1 : 1000; R&D Systems, cat#AF3074).

(2) Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophyFASEB journal : official publication of the Federation of American Societies for Experimental BiologyAugust 1, 2008Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA.Elmer, Boston, MA, USA). Membranes were probed for ang1 with antibodies: [Rockland, Gilbertsville, PA (100-401-403), R&D Systems (MAB050 or AF923), Sigma (A0604), Santa Cruz, Santa Cruz, CA, USA (sc6319), Abcam, Cambridge,

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Acta physiologica (Oxford, England)

Angiopoietin 1 Antibody from Rockland Immunochemicals, Inc.

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Specifications/Features

Rockland Immunochemicals, Inc.

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Angiopoietin 1 Antibody from Rockland Immunochemicals, Inc.

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