FITC anti-mouse IFN-gamma 505806 from BioLegend

Product Specs
ItemFITC anti-mouse IFN-gamma
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number505806
NCBI Gene AliasesIFN-g, If2f, Ifg
Quantity100 µg
CloneXMG1.2
ReactivityMouse
HostRat
Conjugate/TagFITC
IsotypeIgG1, Kappa
ApplicationsIntracellular Staining for Flow Cytometry (ICFC)
Antibody TypePrimary
NCBI Full Gene Nameinterferon gamma
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenE. coli-expressed, recombinant mouse IFN-γ
ClonalityMonoclonal
TargetIFN-gamma

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysisSTAR ProtocolsDecember 1, 2023Rongjing Wang, Minghui Wang, Siyu Pei, Yuchao Zhang, Shiwei Guo, Wei Guo, Zhenchuan Wu, Hailong Wang, Yizhe Li, Yufei Zhu, et al.discard the supernatant completely.g.Incubate the cells with a mouse IFNG-specific antibody (BioLegend, 505806) for 30 min at 4°C. The ratio of the antibody to the cell suspension is 1:200. The concentration of

(2) IFNγ Transcribed by IRF1 in CD4+ Effector Memory T Cells Promotes Senescence-Associated Pulmonary FibrosisAging and diseaseDecember 1, 2023Chen H, Wang Q, Li J, Li Y, Chen A, Zhou J, Zhao J, Mao Z, Zhou Z, Zhang J, et al.CD4-PE (#12-0042-82, eBioscience, USA) and CD44-PE-Cy5 (#15-0441-81, eBioscience, USA), and IFNγ-FITC (#505806, BioLegend, USA) were used. Dylight594-conjugated secondary antibody (goat anti-rabbit IgG, BS10029,

(3) Genetic distinction between functional tissue-resident and conventional natural killer cellsiScienceJuly 21, 2023Luni Hu, Mengwei Han, Yichen Deng, Jingjing Gong, Zhiyuan Hou, Yanyu Zeng, Yime Zhang, Jing He, Chao ZhongAB_2738885 PE/Cyanine7 anti-mouse CD49b,BioLegend,Cat# 103518; RRID: AB_2566103 FITC anti-mouse IFN-γ,BioLegend,Cat# 505806; RRID: AB_315400 APC anti-mouse TNF-α,BioLegend,Cat# 506308; RRID: AB_315429 Brilliant Violet 711™

(4) Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistanceCell reportsApril 25, 2023Gao X, Wu Y, Chick JM, Abbott A, Jiang B, Wang DJ, Comte-Walters S, Johnson RH, Oberholtzer N, Nishimura MI, et al.eBioscience",Thermo Fisher Scientific,Cat# 12–2478-42; RRID:AB_2744700 FITC anti-mouse IFN-gamma Ab,BioLegend,Cat# 505806; RRID:AB_315400 PE anti-mouse IL-2 Ab,BioLegend,Cat# 503808; RRID:AB_315302 FITC anti-human/mouse Granzyme B

(5) Active recruitment of anti–PD-1–conjugated platelets through tumor-selective thrombosis for enhanced anticancer immunotherapyScience AdvancesMarch 31, 2023Yixin Wang, Wen Li, Zhaoting Li, Fanyi Mo, Yu Chen, Mari Iida, Deric L Wheeler, Quanyin Hu(clone no.: 53-6.7, category no.: 100708), and FITC–anti-mouse IFN-γ (clone no.: XMG1.2, category no.: 505806), were purchased from BioLegend. All antibody dilutions were performed according to the manufacturer’s

(6) USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapyNature CommunicationsDecember 1, 2022Wenjun Xiong et al.Perp-Cy5.5-CD45 (30-F11) mAb (103131), PE/Cy7-Granzyme B (QA16A02) mAb (372213), FITC-IFNγ(XMG1.2) mAb (505806), PE/Cy7-CD3666 (TIM3) (RMT3-23) mAb (119715), FITC-H2Kb (AF6-88.5) mAb (116506), APC-H2Kd/ H2Dd (34-1-2

(7) Centrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasisnpj VaccinesDecember 1, 2022Greta Volpedo et al.monoclonal antibodies coupled to fluorescent dyes against these antigens: FITC-IFN-γ (Biolegend Cat#505806, clone: XMG1.2), PerCP/Cy5.5-IL-10 (Biolegend Cat#505027, clone: JES5-16E3), and PE-IL-4 (Biolegend

(8) Depletion of tumor associated macrophages enhances local and systemic platelet-mediated anti-PD-1 delivery for post-surgery tumor recurrence treatmentNature CommunicationsDecember 1, 2022Zhaoting Li, Yingyue Ding, Jun Liu, Jianxin Wang, Fanyi Mo, Yixin Wang, Ting-Jing Chen-Mayfield, Paul M. Sondel, Seungpyo Hong, Quanyin HuGK1.5, 100406), PE-anti-mouse CD8a (BioLegend, 53-6.7, 100708), FITC-anti-mouse IFNγ (BioLegend, XMG1.2, 505806), PerCP/Cy5.5-anti-human/mouse Granzyme B (BioLegend, QA16A02, 372212), PE-anti-mouse CD45 (BioLegend,

(9) Toll-like receptor 9 deficiency induces osteoclastic bone loss via gut microbiota-associated systemic chronic inflammationBone ResearchDecember 1, 2022Peng Ding, Qiyuan Tan, Zhanying Wei, Qiyu Chen, Chun Wang, Luyue Qi, Li Wen, Changqing Zhang, Chen Yaoantibodies used for intracellular staining were purchased from Biolegend and included anti-IFN𝛄-FITC (505806), anti-TNFα-PE (506306) and anti-CD254-PE (510005). Measurements were performed on a CytoFlex flow cytometer

(10) ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progressionNature communicationsNovember 26, 2022Li N, Liu Q, Han Y, Pei S, Cheng B, Xu J, Miao X, Pan Q, Wang H, Guo J, et al.A/E7, 17-4777), Ki67 (BV421, BioLegend; clone: 16A8, 652411) and IFNγ (FITC, BioLegend; clone: XMG1.2, 505806). T cell suppression assay PMN-MDSCs were isolated from 3-month-old PtenPC−/−; Arid1aPC−/− mice prostate

(11) Inhibiting DNA methylation and RNA editing upregulates immunogenic RNA to transform the tumor microenvironment and prolong survival in ovarian cancerJ Immunother CancerNovember 7, 2022Stephanie Gomez, Olivia L Cox, Reddick R Walker, Uzma Rentia, Melissa Hadley, Elisa Arthofer, Noor Diab, Erin E Grundy, Tomas Kanholm, James I McDonald, et al.Staining Buffer Set (Thermo Fisher Scientific, 00-5523-00), and then stained with IFN-γ-FITC (BioLegend, 505806) and FOXp3-AlexaFluor 647 (BD, 563486). Data acquisition was performed on a 3-laser, 12-color FACSCelesta

(12) Nociceptor neurons affect cancer immunosurveillanceNatureNovember 1, 2022Balood M, Ahmadi M, Eichwald T, Ahmadi A, Majdoubi A, Roversi K, Roversi K, Lucido CT, Restaino AC, Huang S, et al.554714) and stained with anti-IFNγ–APC (1:100, BioLegend, 505810), anti-IFNγ–FITC (1:100, BioLegend, 505806), anti-TNF–BV510 (1:100, BioLegend, 506339), anti-TNF–BV5711 (1:100, BioLegend, 506349), anti-TNF–PE

(13) Identification of environmental factors that promote intestinal inflammationNatureNovember 1, 2022Sanmarco LM, Chao CC, Wang YC, Kenison JE, Li Z, Rone JM, Rejano-Gordillo CM, Polonio CM, Gutierrez-Vazquez C, Piester G, et al.anti-IL17a (12-7178-42, BioLegend, 1:100), APCCy7- or FITC-labelled anti-IFNγ (BD Biosciences, and 505806, BioLegend, 1:100), PECy7-labelled anti-hNGFR (345110, BioLegend, 1:100), APC-labelled anti-RORγt (17-6988-82,

(14) Microbial regulation of intestinal motility provides resistance against helminth infectionMucosal ImmunologyNovember 1, 2022Mati Moyat, Luc Lebon, Olaf Perdijk, Lakshanie C. Wickramasinghe, Mario M. Zaiss, Ilaria Mosconi, Beatrice Volpe, Nadine Guenat, Kathleen Shah, Gillian Coakley, et al.performed with Foxp3 staining buffer set (Ebioscience 00-5523-00) and with anti-IFNγ-FITC (Biolegend 505806), anti-IL4-PE (Biolegend 504104) and anti-IL13-biotin (Ebioscience 13-7135-85) overnight. Streptavidin-PECy7

(15) Enhancing Gasdermin-induced tumor pyroptosis through preventing ESCRT-dependent cell membrane repair augments antitumor immune responseNature CommunicationsOctober 24, 2022Zhaoting Li, Fanyi Mo, Yixin Wang, Wen Li, Yu Chen, Jun Liu, Ting-Jing Chen-Mayfield, Quanyin HuGK1.5, 100406), PE-anti-mouse CD8a (BioLegend, 53-6.7, 100708), FITC-anti-mouse IFNγ (BioLegend, XMG1.2, 505806), PerCP/Cy5.5-anti-human/mouse Granzyme B (BioLegend, QA16A02, 372212), PE-anti-mouse CD45 (BioLegend,

(16) Tumorous expression of NAC1 restrains antitumor immunity through the LDHA-mediated immune evasionJournal for ImmunoTherapy of CancerSeptember 23, 2022Yijie Ren et al.the following antibodies: APC-PD-1 (#135210), BV711-TIM3 (#134021), PE-IL-2 (#503808), FITC-IFN-γ (#505806), APC-Thy1.2 (#140331), Pacific Blue-Granzyme B (#515408), PE-TNFα (#506306), PE-CD4 (#100408), APC-perforin

(17) Glucose promotes regulatory T cell differentiation to maintain intestinal homeostasisiScienceSeptember 16, 2022Yu Yu et al.RRID:AB_1107634 Purified anti-mouse CD28(37.51),Bio X Cell,Cat#BE00015-1 anti-IFN-γ-FITC,Biolegend,Cat#505806; RRID:AB_315400 anti-CD4-Brilliant Violet 421,Biolegend,Cat#100544; RRID:AB_11219790 anti-CD4-APC,Biolegend,Cat#100516;

(18) Parvimonas micra promotes colorectal tumorigenesis and is associated with prognosis of colorectal cancer patientsOncogeneSeptember 2, 2022Liuyang Zhao, Xiang Zhang, Yunfei Zhou, Kaili Fu, Harry Cheuk-Hay Lau, Tommy Wai-Yiu Chun, Alvin Ho-Kwan Cheung, Olabisi Oluwabukola Coker, Hong Wei, William Ka-Kei Wu, et al.Biolegend), CD4 (100528, Biolegend), intracellular markers Il-17 (506903, Biolegend) and interferon (IFN)-γ (505806, Biolegend). CD4+ T cells isolation and treatment CD4+ T cells were isolated from mouse spleen by

(19) Intestinal tissue-resident T cell activation depends on metabolite availabilityProceedings of the National Academy of SciencesAugust 23, 2022Špela Konjar, Cristina Ferreira, Filipa Sofia Carvalho, Patrícia Figueiredo-Campos, Júlia Fanczal, Sofia Ribeiro, Vanessa Alexandra Morais, Marc Veldhoen,BD Bioscience,561126,https://www.bdbiosciences.com/en-us/products/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/alexa-fluor-647-mouse-anti-ki-67.561126 IFNγ,FITC,XMG1.2,BioLegend,505806,https://www.biolegend.com/en-us/products/fitc-anti-mouse-ifn-gamma-antibody-995 IFNγ,APC,XMG1.2,BioLegend,505810,https://www.biolegend.com/en-us/products/apc-anti-mouse-ifn-gamma-antibody-993?GroupID=GROUP24 Flow cytometry

(20) Succinate uptake by T cells suppresses their effector function via inhibition of mitochondrial glucose oxidationCell reportsAugust 16, 2022Gudgeon N, Munford H, Bishop EL, Hill J, Fulton-Ward T, Bending D, Roberts J, Tennant DA, Dimeloe S.being stained for 20 min with anti-IFN-γ and anti-TNF-α (anti-IFN-γ-FITC, Biolegend, Clone XMG1.2, Cat# 505806; anti-TNF-α-PE, Biolegend, Clone MP6-XT22, Cat# 506305) and then undergoing further washing and analysis. Multiplex

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(1)

1/4/2024Anti-Mouse IFNg FITC Antibody Produces Satisfactory Results Tin NgoWe use this antibody to stain for IFNgamma cytokine in T cells after ex vivo stimulation. It works well and we never have issues with this antibody.
(1)

3/28/2019Flow Cytometry Antibody for Mouse IFN-g Prabhakaran KumarA cheap antibody, worth its price.
(1)

2/21/2017IFNgamma FITC Antibody Maria Carmela SperanzaSplenocytes extracted from brain tumor-bearing mice, gated for live cells, CD45+, CD3+, CD8+ and IFNg. The experiment was perform to evaluate the release of IFNg after treatment.The Ab was choose for ... Read Review

FITC anti-mouse IFN-gamma from BioLegend

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FITC anti-mouse IFN-gamma from BioLegend

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