FITC anti-mouse CD4 100406 from BioLegend

Product Specs
ItemFITC anti-mouse CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100406
NCBI Gene AliasesL3T4, Ly-4
IsotypeIgG2b, Kappa
CloneGK1.5
Quantity500 µg
ApplicationsFlow Cytometry (FC), Immunohistochemistry (IHC)-F, Immunocytochemistry (ICC)
ReactivityMouse
Conjugate/TagFITC
HostRat
Antibody TypePrimary
NCBI Full Gene NameCD4 antigen
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenMouse CTL clone V4
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A HER2-targeting antibody-MMAE conjugate RC48 sensitizes immunotherapy in HER2-positive colon cancer by triggering the cGAS-STING pathwayCell death & diseaseAugust 24, 2023Wu X, Xu L, Li X, Zhou Y, Han X, Zhang W, Wang W, Guo W, Liu W, Xu Q, et al.Biolegend) for dead cell preclusion, CD45-BV510 (103138, Biolegend), CD3-APC (100236, Biolegend), CD4-FITC (100406, Biolegend), CD8-BV421 (100738, Biolegend), IFN-γRα-PE (308606, Biolegend). IHC and TUNEL assays The

(2) Roquin-1 resolves sepsis-associated acute liver injury by regulating inflammatory profiles via miRNA cargo in extracellular vesiclesiScienceAugust 18, 2023Zheng L, Ling W, Zhu D, Li Z, Li Y, Zhou H, Kong L.PerCP-Cy5.5,Biolegend,117327 anti-CD11b PE-Cy7,Biolegend,101215 anti-CD206 APC,Biolegend,141707 anti-CD4 FITC,Biolegend,100405 anti-CD25 APC-Cy5.5,Biolegend,162105 anti-CD45-FITC,Biolegend,304006 anti-CD11b-APC,Biolegend,301410 anti-CD14-APC-Cy7,Biolegend,367108 anti-CCR2-BV605,Biolegend,357214 anti-mouse

(3) Butyrate Protects against Clostridium difficile Infection by Regulating Bile Acid MetabolismMicrobiology spectrumAugust 17, 2023Wang S, Xiang L, Li F, Deng W, Lv P, Chen Y.single-cell suspension and then stained with an FITC anti-mouse CD4 antibody (BioLegend, San Diego, CA; 100406), Alexa Fluor 647 rat anti-mouse IL-17A antibody (BD Biosciences, Franklin Lakes, NJ; 560224), or antigen-presenting cell

(4) RORγt-Raftlin1 complex regulates the pathogenicity of Th17 cells and colonic inflammationNat CommunAugust 17, 2023Amir Kumar Singh, Ritesh Kumar, Jianyi Yin, John F. Brooks Ii, Mahesh Kathania, Sandip Mukherjee, Jitendra Kumar, Kevin P. Conlon, Venkatesha Basrur, Zhe Chen, et al.Fluor® 48 (E11, Santa Cruz biotechnology, sc-514457 AF488, 1:100), Anti-CD4-FITC (GK1.5, Biolegend, 100406, 1:200), Anti-CD4-PE (GK1.5, BD Bioscience, 553730, 1:200), Anti-CD4-APC (GK1.5, eBioscience, 17004181,

(5) A rapid cell-free expression and screening platform for antibody discoveryNature communicationsJuly 3, 2023Hunt AC, Vögeli B, Hassan AO, Guerrero L, Kightlinger W, Yoesep DJ, Krüger A, DeWinter M, Diamond MS, Karim AS, et al.FACS buffer followed by staining with anti-CD19 BV421 (BioLegend # 115537), anti-CD4 FITC (BioLegend # 100405), anti-IgD-PE-Cy7 (BioLegend # 405719), Streptavidin APC (BioLegend # 405207), aqua cell viability dye

(6) T cell infiltration into the brain triggers pulmonary dysfunction in murine Cryptococcus-associated IRISNature communicationsJune 28, 2023Kawano T, Zhou J, Anwar S, Salah H, Dayal AH, Ishikawa Y, Boetel K, Takahashi T, Sharma K, Inoue M.BioLegend), PE/Cy5 CD3 (Ref# 15–0031, eBioscience), PE/Cy7 CD4 (Cat# 100422, BioLegend), FITC CD4 (Cat# 100406, BioLegend), PE/Cy7 CD19 (Cat# 115520, BioLegend), PE/Cy7 CD11c (Cat# 117318, BioLegend), Alexa647 F4/80

(7) A SARS-CoV-2 and influenza double hit vaccine based on RBD-conjugated inactivated influenza A virusScience advancesJune 23, 2023Wang Z, Li Z, Shi W, Zhu D, Hu S, Dinh PC, Cheng K.Following that, the splenocytes were collected and stained with CD4–fluorescein isothiocyanate (FITC) (100406, BioLegend) antibody or CD8-FITC (ab22504, Abcam) antibody at a dilution of 1:100. After washing with

(8) Tryptophan depletion sensitizes the AHR pathway by increasing AHR expression and GCN2/LAT1-mediated kynurenine uptake, and potentiates induction of regulatory T lymphocytesJournal for ImmunoTherapy of CancerJune 21, 2023Marie Solvay, Pauline Holfelder, Simon Klaessens, Luc Pilotte, Vincent Stroobant, Juliette Lamy, Stefan Naulaerts, Quentin Spillier, Raphaël Frédérick, Etienne De Plaen, et al.viability dye eflour780 (1/2000) (#65-0865-14, ThermoFisher) and anti CD4 antibody-FITC (clone GK1.5, #100406, Biolegend) (1/200) and then fixed and permeabilized with the intracellular fixation/permeabilisation

(9) Fcγ receptor-dependent antibody effector functions are required for vaccine protection against infection by antigenic variants of SARS-CoV-2bioRxivMay 8, 2023Samantha R. Mackin, Pritesh Desai, Bradley M. Whitener, Courtney E. Karl, Meizi Liu, Ralph S. Baric, Darin K. Edwards, Taras M. Chicz, Ryan P. McNamara, Galit Alter, et al.BD Biosciences), CD8β (PerCP/Cy5.5; Clone YTS156.7.7; Cat: 126610; BioLegend), CD4 (FITC; Clone GK1.5; Cat: 100406; BioLegend), CD44 (APC/Cy7; Clone IM7; Cat: 103028; BioLegend) for 30 min at 4°C in FACS buffer (1x

(10) Temperature sensitive liposome based cancer nanomedicine enables tumour lymph node immune microenvironment remodellingNature CommunicationsApril 19, 2023Shunli Fu, Lili Chang, Shujun Liu, Tong Gao, Xiao Sang, Zipeng Zhang, Weiwei Mu, Xiaoqing Liu, Shuang Liang, Han Yang, et al.100236; Clone name: 17A2; 1:100 dilution), FITC anti-mouse CD4 Antibody (BioLegend; Catalog number: 100405; Clone name: GK1.5; 1:400 dilution), PE anti-mouse CD8a Antibody (BioLegend; Catalog number: 100708;

(11) Targeting DCLK1 attenuates tumor stemness and evokes antitumor immunity in triple-negative breast cancer by inhibiting IL-6/STAT3 signalingBreast cancer research : BCRApril 17, 2023Liu H, Yan R, Xiao Z, Huang X, Yao J, Liu J, An G, Ge Y.Violet 421 anti-mouse CD3 Antibody (1:500, Biolegend, 100227), FITC anti-mouse CD4 (1:500, Biolegend, 100406), PE anti-mouse CD8a (1:500, Biolegend, 100708), 7-AAD Viability Staining Solution (1:500, BD, 559925). Immunohistochemistry The

(12) EHBP1L1 Drives Immune Evasion in Renal Cell Carcinoma through Binding and Stabilizing JAK1Advanced science (Weinheim, Baden-Wurttemberg, Germany)April 1, 2023Pan Y, Shu G, Fu L, Huang K, Zhou X, Gui C, Liu H, Jin X, Chen M, Li P, et al.anti‐human/mouse Granzyme B (372207, Biolegend), PE anti‐mouse CD45 (147711, Biolegend), FITC anti‐mouse CD4 (100405, Biolegend), Alexa Fluor 700 anti‐mouse FOXP3 (126421, Biolegend), APC anti‐mouse CD19 (152409, Biolegend),

(13) Carbon ion radiotherapy triggers immunogenic cell death and sensitizes melanoma to anti-PD-1 therapy in miceOncoImmunologyDecember 31, 2022Heng Zhou et al.for 30 min. The antibodies FITC-CD11b (#101206), APC-GR1 (#108412), FITC-CD3 (#100204), FITC-CD4 (#100406), and APC-CD8b.2 (#140410) were purchased from BioLegend (San Diego, CA, USA). Flow cytometry was conducted

(14) FOXO1 Alleviates Liver Ischemia-reperfusion Injury by Regulating the Th17/Treg Ratio through the AKT/Stat3/FOXO1 PathwayJournal of Clinical and Translational HepatologyDecember 28, 2022Hao-zhen Ren, Sen-zhe Xia, Xue-qian Qin, An-yin Hu, Jing-lin Wangagainst mouse lymphocyte surface markers were: fluoresceine isothiocyanate-conjugated anti-mouse CD4 (100405; Biolegend, San Diego, CA, USA), allophycocyanin-conjugated anti-mouse CD3 (100235; Biolegend, San Diego,

(15) Directly targeting ASC by lonidamine alleviates inflammasome-driven diseasesJournal of neuroinflammationDecember 28, 2022Chen C, Zhou Y, Ning X, Li S, Xue D, Wei C, Zhu Z, Sheng L, Lu B, Li Y, et al.flow cytometric analysis. The following antibodies were used: CD45-BV510 (BD, 563891), CD4-FITC (BioLegend, 100406), CD8-Alexa Fluor 700 (BD, 557959), and CD11b-BV421 (BioLegend, 101236). Flow cytometry analysis was

(16) A mitochondria-anchored supramolecular photosensitizer as a pyroptosis inducer for potent photodynamic therapy and enhanced antitumor immunityJournal of NanobiotechnologyDecember 3, 2022Hong Wang, Guoxin Jing, Jintong Niu, Li Yang, Youyuan Li, Yi Gao, Huichao Wang, Xiaorong Xu, Yechang Qian, Shilong Wangand further labeled with fluorochrome-conjugated antibody CD45 (Biolegend, 103112), CD4 (Biolegend, 100405), CD8 (Biolegend, 100707), CD80 (Biolegend, 104705) and CD86 (Biolegend, 159203) respectively following

(17) Precision cancer sono-immunotherapy using deep-tissue activatable semiconducting polymer immunomodulatory nanoparticlesNature CommunicationsDecember 1, 2022Jingchao Li, Yu Luo, Ziling Zeng, Dong Cui, Jiaguo Huang, Chenjie Xu, Liping Li, Kanyi Pu, Ruiping Zhangclone PO3), CD3-APC/Cyanine7 (Biolegend, cat. no. 100222, clone 17A2), CD4-FITC (Biolegend, cat. no. 100406, clone GK1.5), CD8-PE (Biolegend, cat. no. 140408, clone 53-5.8), Foxp3-PE (Biolegend, cat. no. 126404,

(18) Sirt6-mediated epigenetic modification of DNA accessibility is essential for Pou2f3-induced thymic tuft cell developmentCommunications BiologyDecember 1, 2022Qian Zhang, Jiayu Zhang, Tong Lei, Zhanfeng Liang, Xue Dong, Liguang Sun, Yong Zhao(eBioscience, clone 4B10, 12-5825-82, 1:200 dilution); Lin--FITC: CD4-FITC (Biolegend, clone GK1.5, 100405, 1:1000 dilution); CD8-FITC (BD Biosciences, clone 53-6.7, 553031, 1:400 dilution); CD19-FITC (Biolegend,

(19) Toll-like receptor 9 deficiency induces osteoclastic bone loss via gut microbiota-associated systemic chronic inflammationBone ResearchDecember 1, 2022Peng Ding, Qiyuan Tan, Zhanying Wei, Qiyu Chen, Chun Wang, Luyue Qi, Li Wen, Changqing Zhang, Chen Yao(128013), anti-Ly-6G-Pacific Blue (127611), anti-lineage cocktail-Pacific Blue™ (133305), anti-CD4-FITC (100405), anti-CD16/32-FITC (101305), anti-CD4-PE (100407), anti-CD19-PE (115507), anti-CD115-PE (135505), anti-CD127-PE

(20) Depletion of tumor associated macrophages enhances local and systemic platelet-mediated anti-PD-1 delivery for post-surgery tumor recurrence treatmentNature CommunicationsDecember 1, 2022Zhaoting Li, Yingyue Ding, Jun Liu, Jianxin Wang, Fanyi Mo, Yixin Wang, Ting-Jing Chen-Mayfield, Paul M. Sondel, Seungpyo Hong, Quanyin HuM1/70, 101227), APC-anti-mouse CD3 (BioLegend, 17A2, 100236), FITC-anti-mouse CD4 (BioLegend, GK1.5, 100406), PE-anti-mouse CD8a (BioLegend, 53-6.7, 100708), FITC-anti-mouse IFNγ (BioLegend, XMG1.2, 505806),

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(1)

4/23/2020CD4 T Cell Detection - FITC Preeyam PatelEasy fit into panels and simple detection.
(1)

8/22/2019Review of Anti-Mouse CD4 (BioLegend) Sreya BagchiGood antibody for staining mouse CD4+ cells.
(1)

7/22/2016Good Separation Using This Antibody Jackie Lee.This antibody was used to gate on the CD4 T cells in a mixed population after in vitro culture.

FITC anti-mouse CD4 from BioLegend

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FITC anti-mouse CD4 from BioLegend

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