Anti-Angiopoietin 1 antibody ab8451 from Abcam

Product Specs
ItemAnti-Angiopoietin 1 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab8451
Antibody TypePrimary
HostRabbit
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
ClonalityPolyclonal
Quantity200 UL
IsotypeIgG
Conjugate/TagUnconjugated
Concentration85 mg/ml
Gene NameANGPT1
ReactivityAfrican green monkey, Human, Mouse
TargetAngiopoietin 1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Characterization of the Ang/Tie2 Signaling Pathway in the Diaphragm Muscle of DMD MiceBiomedicinesAugust 14, 2023Lin Y, McClennan A, Hoffman L.with continuous agitation. After blocking, membranes were incubated with primary anti-Ang1 (Abcam, ab8451, 1:1000, Cambridge, United Kingdom), Ang2 (Invitrogen, PA5–27297, 1:1000, Waltham, MA, USA), AKT (Cell Signaling

(2) Utilizing Contrast-Enhanced Ultrasonography with Phosphatidylserine Microbubbles to Detect Placental Inflammation in Rhesus MacaquesMoleculesMarch 23, 2023Rachel C. Wilson, Jamie O. Lo, Gabriel Romero Jimenez, Jonathan R. Lindner, Ov D. Slayden, Victoria H. J. Roberts°C (Selectin p: 1:200 dilution, Abcam, Cat# ab6632, Waltham, MA, USA; ANGPT1: 1:800 dilution, Abcam, ab8451; ANGPT2: 1:800 dilution, Abcam, ab56301). We stained a separate, corresponding section from each tissue

(3) Effects of swimming before and during pregnancy on placental angiogenesis and perinatal outcome in high-fat diet-fed micePeerJFebruary 20, 2023Xiaofeng Zhu, Weiwei Chen, Haitang Wangagainst PPARγ (CST, #2443, 53 kDa, 1:1000), PPARα (ab3484, 52 kDa, 1:1000; Abcam, Cambridge, UK), ANGPT1 (ab8451, 57 kDa, 1:500; Abcam, Cambridge, UK), VEGF (CY2367, 28 kDa, 1:1000; Abways, Kärnten, Austria), β-actin

(4) Intrarenal Single-Cell Sequencing of Hepatitis B Virus Associated Membranous NephropathyFrontiers in MedicineJuly 22, 2022Leilin Yu et al.(GB11310, Wuhan Servicebio Technology, 1:5000), HSPA5 (GB11492, Wuhan Servicebio Technology, 1:50), IGFBR7 (ab8451, Abcam, 1:3000), JUN (ab8451, Abcam, 1:500), SELE (GB113279, Wuhan Servicebio Technology, 1:100), TNFAIP3

(5) Endothelial-mesenchymal transition induced by metastatic 4T1 breast cancer cells in pulmonary endothelium in aged miceFrontiers in molecular biosciencesJanuary 1, 2022Smeda M, Jasztal A, Maleki EH, Bar A, Sternak M, Kwiatkowski G, Suraj-Prażmowska J, Proniewski B, Kieronska-Rudek A, Wojnar-Lason K, et al.eNOS (1:1000; 610296; BD Transduction Laboratories; Franklin Lakes, NJ, United States), Ang-1 (1:1000; ab8451; Abcam), Ang-2 (1:10000; PA5-27297; Thermo Fisher Scientific), HIF1α (5 μg/ml; ab1; Abcam), VE-CAD (1:1000;

(6) The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous mannerCell reportsAugust 17, 2021Luck R, Karakatsani A, Shah B, Schermann G, Adler H, Kupke J, Tisch N, Jeong HW, Back MK, Hetsch F, et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER ,, Antibodies,, ,, Rabbit polyclonal anti-Ang1,Abcam,ab8451 anti-chicken-Alexa488,Molecular Probes,A11039 anti-goat-Alexa488,Jackson ImmunoResearch,705–545-033 anti-goat-Alexa568,Molecular Probes

(7) The Inhibitory Effect of Lysophosphatidylcholine on Proangiogenesis of Human CD34+ Cells Derived Endothelial Progenitor CellsFrontiers in Molecular BiosciencesJune 10, 2021Haijun Zhao, Yanhui He1/ 1,000, Abcam), anti-IL-8 (#ab235584, 1/1,000, Abcam), anti-VEGF (#ab1316, 1/2000, Abcam), anti-ANG-1 (#ab8451, 1/500, Abcam), anti-ANG-2 (#ab155106, 1/500, Abcam), anti-TGF-β1 (#ab215715, 1/ 1,000, Abcam), anti-p-PKC

(8) Overexpression of COMP-Angiopoietin-1 in K14-Expressing Cells Impairs Hematopoiesis and Disturbs Erythrocyte MaturationMolecules and CellsApril 30, 2021Hyun-Jaung Sim, Min-Hye Kim, Govinda Bhattarai, Jae-Won Hwang, Han-Sol So, Sher Bahadur Poudel, Eui-Sic Cho, Sung-Ho Kook, Jeong-Chae Lee(cat.#sc-28876, 1:50; Santa Cruz Biotechnology), mouse-anti-CD31 (cat.#ab9498; Abcam, UK), anti-Ang1 (cat.#ab8451; Abcam), or with anti-vascular endothelial growth factor (VEGF) (cat.#BS2853; Bioworld Technology, USA)

(9) Interleukin-6 as an enhancer of anti-angiogenic therapy for ovarian clear cell carcinomaScientific reportsApril 8, 2021Seki T, Yanaihara N, Shapiro JS, Saito M, Tabata J, Yokomizo R, Noguchi D, Kuroda T, Kawabata A, Suzuki J, et al.retrieval, and then incubated in antibodies against IL-6 (1:400, 21865-1-AP; Proteintech) and Ang1 (1:200, ab8451; Abcam, Cambridge, UK). Immunostained slides were evaluated by two independent pathologists (H.M and

(10) Exosomes from microRNA-126 overexpressing mesenchymal stem cells promote angiogenesis by targeting the PIK3R2-mediated PI3K/Akt signalling pathwayJournal of cellular and molecular medicineFebruary 1, 2021Zhang L, Ouyang P, He G, Wang X, Song D, Yang Y, He X.(1:1000; Cell Signaling Technology; #2171), anti‐VEGF (1:1000; Abcam; ab46154), anti‐Ang‐1 (1:500; Abcam; ab8451), anti‐PIK3R2 (1:1000; Abcam; ab131067), anti‐phosphorylated Akt (p‐Akt; 1:1000, Cell Signaling Technology;

(11) LncRNA NEAT1 promotes proliferation of ovarian cancer cells and angiogenesis of co-incubated human umbilical vein endothelial cells by regulating FGF9 through sponging miR-365: An experimental studyMedicine (Baltimore)January 22, 2021Jialing Yuan, Ke Yi, Lingyun YangPrimary antibodies of β-actin (ab8226, 1:1000), FGF9 (ab206408, 1:1000), VEGF (ab69479, 1:1000), Ang-1 (ab8451, 1:500), or MMP2 (ab97779, 1:500) (Abcam, Cambridge, MA, USA) were incubated with the proteins on a

(12) Cryptotanshinone enhances wound healing in type 2 diabetes with modulatory effects on inflammation, angiogenesis and extracellular matrix remodellingPharmaceutical BiologySeptember 2, 2020Min Song, Lu Chen, Lusha Zhang, Chunxiao Li, Joel Wake Coffie, Zhirui Fang, Liyuan Zhang, Shaoxia Wang, Xiumei Gao, Hong Wang(Shenyang, China). Other antibodies including VEGF (ab46154), CD45 (ab10558), Angiopoietin-1 (Ang-1, ab8451), matrix metallopeptidase 9 (MMP9, ab38898), α-smooth muscle actin (α-SMA, ab5694) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594

(13) Endostar blocks the metastasis, invasion and angiogenesis of ovarian cancer cellsNeoplasmaJanuary 1, 2020Y. Ding, Y. Wang, J. Cui, T. Siangiogenesis-related proteins (EGFR (1:1000, ab52894, Abcam, USA), bFGF (0.5 µg, ab126861, Abcam, USA), ANG1 (1:500, ab8451, Abcam, USA), PD-L1 (2 µg/ml, ab205921, Abcam, USA), p-STAT3 (1:2000, #9145, Cell Signaling Technology,

(14) Hematoma-derived exosomes of chronic subdural hematoma promote abnormal angiogenesis and inhibit hematoma absorption through miR-144-5pAgingDecember 16, 2019Gao C, Gong Z, Wang D, Huang J, Qian Y, Nie M, Jiang W, Liu X, Luo H, Yuan J, et al.concentrations of ANG-1 and ANG-2 in HUVECs were evaluated by western blotting using rabbit anti-ANG-1 (ab8451, Abcam, Cambridge, UK, 1:500), rabbit anti-ANG-2 (ab8452, Abcam, Cambridge, UK, 1:500) antibodies, and

(15) lncRNA ATXN8OS promotes breast cancer by sequestering miR‑204Molecular Medicine ReportsJune 6, 2019Zhen Deng, Huayu Cai, Liying Lin, Lingfeng Zhu, Weizhen Wu, Shunliang Yang, Jinquan Cai, Jianming Tan(1:1,000; cat. no. 53528; Cell Signaling Technology, Inc.), angiopoietin 1 (ANGPT1; 1:500; cat. no. ab8451; Abcam), TGF-β receptor type 2 (TGFβR2; 1:500; cat. no. ab186838; Abcam) and GAPDH (1:1,000; cat. no.

(16) Embossed Membranes with Vascular Patterns Guide Vascularization in a 3D Tissue ModelPolymers (Basel)May 2, 2019Soyoung Hong, Eun Young Kang, Jaehee Byeon, Sung-ho Jung, Changmo Hwangmarker Anti-VE-cadherin poly 1:100 Abcam Ab33168 Anti-CD31 mono 1:100 Thermo MA3100 Endothelial cell marker Anti-Ang-1 poly 1:100 Abcam Ab8451 Vascularization Anti-VEGF mono 1:100 Abcam Ab1316 Vascularization Anti-Human mitochondria mono 1:100 Sigma MAB1273 Human

(17) Biopolymer-delivered vascular endothelial growth factor improves renal outcomes following revascularizationAmerican journal of physiology. Renal physiologyMay 1, 2019Guise E, Engel JE, Williams ML, Mahdi F, Bidwell GL 3rd, Chade AR.(ab18976; Abcam), VEGF (ab53465; Abcam), Flk-1 (sc-505; Santa Cruz Biotechnology), angiopoietin-1 (Ang-1, ab8451; Abcam), stromal-derived factor-1 (SDF-1, AVARP07031-P050; Aviva Systems Biology, San Diego, CA), CXCR-4

(18) Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in miceBreast cancer research : BCRAugust 3, 2018Smeda M, Kieronska A, Adamski MG, Proniewski B, Sternak M, Mohaissen T, Przyborowski K, Derszniak K, Kaczor D, Stojak M, et al.eNOS (610,296; BD Transduction Laboratories), VEGFA (ab68334; Abcam), VEGFR2 (ab39256; Abcam), Ang-1 (ab8451; Abcam), Ang-2 (PA5-27297; Thermo Fisher Scientific), VCAM-1 (CBL1300; Merck), Slit2 (ab134166; Abcam),

(19) Targeting Tie-2/angiopoietin axis in experimental mesothelioma confers differential responses and raises predictive implicationsOncotargetApril 24, 2018Sophia Magkouta, Apostolos Pappas, Ioannis S. Pateras, Androniki Kollintza, Charalampos Moschos, Maria-Eleni Vazakidou, Vasiliki Karavana, Vassilis G. Gorgoulis, Ioannis KalomenidisPathology, Evangelismos Hospital, Greece. Mesothelioma tumor tissue sections were stained for Ang-1 (ab8451) and -2 (ab153934) (Abcam, Cambridge, UK) and staining was evaluated by an experienced pathologist.

(20) Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumourOncotargetApril 3, 2018Smeda M, Kieronska A, Proniewski B, Jasztal A, Selmi A, Wandzel K, Zakrzewska A, Wojcik T, Przyborowski K, Derszniak K, et al.primary antibodies directed against the following antigens: MMP-2 (ab19167), MMP-9 (ab19016), Ang-1 (ab8451), TSP-1 (ab85762), TGFβ1 (ab155264), VEGF(A) (ab68334), PDGF AB+BB (ab34074), vWF (ab9378), Slit2 (ab134166)

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8/23/2023State of the Art Antibody for the Immunodetection of Angiopoietin 1 in Human Primary Cells Raj KumarCells from the human cell line were exposed to a cell growth factor, and cell extract was prepared. The extracted protein was quantified by using a BCA reagent, and an equal amount of proteins from ... Read Review

Anti-Angiopoietin 1 antibody from Abcam

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Anti-Angiopoietin 1 antibody from Abcam

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