Anti-Collagen IV antibody [COL-94] ab6311 from Abcam

Product Specs
ItemAnti-Collagen IV antibody [COL-94]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab6311
Antibody TypePrimary
Quantity50 UL
CloneCOL-94
ClonalityMonoclonal
IsotypeIgG1
ApplicationsDot Blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration0.1 mg/ml
Gene NameCol4a1
ReactivityHuman
TargetCollagen IV

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Characterization of extracellular matrix deposited by segmental trabecular meshwork cellsbioRxivMarch 16, 2023VijayKrishna Raghunathan, Andrews Nartey, Kamesh Dhamodaran, Hasna Baidouri, Julia A. Staverosky, Kate E Keller, Keith Zientek, Ashok Reddy, Ted Acott, Janice A VrankapH 7.4) and PBS, blocked with 3% fish gelatin, and immunolabelled with anti-collagen IV (catalog # ab6311, Abcam, Cambridge, MA, USA), anti-laminin (catalog # ab11575, Abcam, Cambridge, MA, USA) or anti-Fibronectin

(2) Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processesAPL BioengineeringMarch 1, 2023Hannah M. Micek, Lauren Rosenstock, Yicheng Ma, Caitlin Hielsberg, Lauren Montemorano, Metti K. Gari, Suzanne M. Ponik, Pamela K. Kreegerμg/ml), FSP1 (Sigma-Aldrich 07–2274, 10 μg/ml), collagen I (Abcam ab34710, 1:500), collagen IV (Abcam ab6311, 1:200), laminin (Abcam ab11575, 1:400), fibronectin (Abcam ab6328, 1:200), PAX8 (PA1–112, 1:300), vimentin

(3) Space Omics and Tissue Response in Astronaut Skeletal Muscle after Short and Long Duration MissionsInternational Journal of Molecular SciencesFebruary 17, 2023Dieter Blottner, Manuela Moriggi, Gabor Trautmann, Maria Hastermann, Daniele Capitanio, Enrica Torretta, Katharina Block, Joern Rittweger, Ulrich Limper, Cecilia Gelfi, et al.rabbit monoclonal. 1:2000, Abcam ab 199720), anti-collagen type 6 (mouse monoclonal, 1:2000, Abcam ab6311), perlecan (rat IgG2a, 1:400, Invitrogen MA-06821), and matrix metalloproteinase 2 (1:100), with secondary

(4) The Role of CD200–CD200 Receptor in Human Blood and Lymphatic Endothelial Cells in the Regulation of Skin Tissue InflammationCellsMarch 21, 2022Dominic Rütsche, Katarzyna Michalak-Micka, Dominika Zielinska, Hannah Moll, Ueli Moehrlen, Thomas Biedermann, Agnes S. KlarSwitzerland",1:50 CD200R,OX-108,329302,"BioLegend, Switzerland",1:50 CD200R-APC,OX-108,329308,"BioLegend, Switzerland",1:20 Collagen IV,COL-94,ab6311,"Abcam, UK",1:200 Ki67,B56,550609,"BD Biosciences, Switzerland",1:100 LYVE1,polyclonal,Ab10278,"Abcam,

(5) Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation cultureRegenerative therapyMarch 1, 2022Yamasaki S, Kuwahara A, Kishino A, Kimura T, Takahashi M, Mandai M.,Santa Cruz Biotechnology,sc-365519,AB_10842442,1:500 Chx10,Sheep,Exalpha Biologicals,X1180P,AB_2314191,1:500 Collagen-IV,Rabbit,Abcam,ab6311,AB_305414,1:500 Crx,Rabbit,Takara Bio Inc.,M231,,1:500 EphrinB2,Goat,R&D Systems,AF496,AB_2095679,1:500 Laminin,Rabbit,Abcam,ab-11575,AB_298179,1:500 Mitf,Mouse,Exalpha

(6) Epithelial phenotype restoring drugs suppress macular degeneration phenotypes in an iPSC modelNature communicationsDecember 15, 2021Sharma R, George A, Nimmagadda M, Ortolan D, Karla BS, Qureshy Z, Bose D, Dejene R, Liang G, Wan Q, et al.ZO-1 (5 µg/mL; MA3-39100-A488, ThermoFisher), EZRIN (1 µg/mL; E8897, Sigma), COLLAGEN IV (~5 µg/mL; ab6311, Abcam), C5aR1 (~1 µg/mL; ab11867, Abcam), C3aR1 (~1 µg/mL; 126250, Abcam), RELB (~2 µg/mL; 4922, CST),

(7) Serum Anti-Collagen IV IgM and IgG Antibodies as Indicators of Low Vascular Turnover of Collagen IV in Patients with Long-Term Complications of Type 2 DiabetesDiagnostics (Basel)May 19, 2021Krasimir Kostov, Alexander Blazhevwas sensitized with 100 µL of 10 µg/mL of mouse monoclonal antibody to collagen IV [COL-94] (Cat. No. ab6311, Abcam, Cambridge, UK), followed by an overnight incubation at 4 ◦ C. Then, 100 µL serum sample (diluted

(8) The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3DNature protocolsApril 1, 2021Rodrigues Toste de Carvalho AL, Liu HY, Chen YW, Porotto M, Moscona A, Snoeck HW.3267,RRID:AB_2275453,https://scicrunch.org/resolver/RRID:AB_2275453,1:400 COL4,mouse,unconjugated,COL-94,Abcam Cat# ab6311,RRID:AB_305414,https://scicrunch.org/resolver/RRID:AB_305414,1:200 HN,rat,unconjugated,,"Moscona Lab

(9) Piezo2 downregulation via the Cre-lox system affects aqueous humor dynamics in miceMolecular visionJanuary 1, 2021Fang J, Hou F, Wu S, Liu Y, Wang L, Zhang J, Wang N, Wang K, Zhu W.antibody anti-Piezo2 (NBP1–78624, Novus, Littleton, CO), mouse polyclonal antibodies anti-Collagen IV (ab6311) and GFAP (ab10062, Abcam, Cambridge, MA), and mouse monoclonal antibody anti-SNCG (MAB5745, Novus).

(10) Pancreas Optical Clearing and 3-D Microscopy in Health and DiabetesFrontiers in endocrinologyJanuary 1, 2021Campbell-Thompson M, Tang SC.(arteries) Rabbit Mouse Mouse Mouse Abcam ThermoFisher ThermoFisher Abcam Ab28364 MS-353-S1 MA1-10202 ab6311 1:30 1:50 1:50 1:200 Worked Worked, weak staining Did not work Did not work Rabbit Abcam ab6586 1:200 Worked Mouse Sigma A5228;

(11) Dexamethasone and Glucocorticoid-Induced Matrix Temporally Modulate Key Integrins, Caveolins, Contractility, and Stiffness in Human Trabecular Meshwork CellsInvestigative Opthalmology & Visual ScienceNovember 10, 2020Felix Yemanyi, Hasna Baidouri, Alan R. Burns, VijayKrishna Raghunathananti-fibronectin extra domain B (ED-B; catalog number: ab154210; Abcam), anti-collagen IV (catalog number: ab6311; Abcam), respectively, at 1/250 dilution in 5% BSA/PBS (primary antibodies were omitted as negative

(12) Glucocorticoid-induced cell-derived matrix modulates transforming growth factor β2 signaling in human trabecular meshwork cellsScientific reportsSeptember 24, 2020Yemanyi F, Vranka J, Raghunathan VK.anti-Fibronectin (catalog number: ab6584, Abcam, Cambridge, MA, USA) and anti-Collagen IV (catalog number: ab6311, Abcam, Cambridge, MA, USA) respectively at 1/250 dilution in 5% BSA/PBS (Primary anti-bodies were omitted

(13) CXCL12γ induces human prostate and mammary gland developmentThe ProstateSeptember 1, 2020Younghun Jung, Jin Koo Kim, Eunsohl Lee, Frank C. Cackowski, Ann M. Decker, Paul H. Krebsbach, Russell S. TaichmanFisher Scientific, Fremont, CA), CK5 (cat. ab52635, Abcam), CD44 (cat. ab6124, Abcam), Collagen IV (cat. ab6311, Abcam), SYN (cat. ab23754, Abcam), and human leukocyte antigen (HLA, cat. ab70328, Abcam) antibodies

(14) Crosslinked Extracellular Matrix Stiffens Human Trabecular Meshwork Cells Via Dysregulating β-catenin and YAP/TAZ Signaling PathwaysInvestigative Opthalmology & Visual ScienceAugust 25, 2020Felix Yemanyi, Janice Vranka, Vijay Krishna Raghunathanab11575; Abcam), anti-Fibronectin (catalog number: ab2413; Abcam) or anti-Collagen IV (catalog number: ab6311; Abcam), respectively, at 1/250 dilution in 5% BSA/PBS (primary antibodies were omitted as negative

(15) Use of Glycated Hemoglobin (A1c) as a Biomarker for Vascular Risk in Type 2 Diabetes: Its Relationship with Matrix Metalloproteinases-2, -9 and the Metabolism of Collagen IV and ElastinMedicina (Kaunas)May 11, 2020Krasimir Kostov, Alexander Blazhevwas sensitized with 100 µL of 10 µg/mL of mouse monoclonal antibody to collagen IV (COL-94) (Cat. No. ab6311, Abcam, Cambridge, UK) at room temperature for 3 h, followed by overnight incubation at 4 ◦ C. The plate

(16) Патофизиологические механизмы эпителиально-мезенхимальной трансформации при идиопатическом эпиретинальном фиброзеZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia»March 31, 2020S.A. Borzenok, V.D. Zakharov, A.V. Miridonova, A. Kupriyanova, S.V. Kolesnik, D.S. Ostrovsky, I.M. Gorshkov, A.I. Kolesnik, P.M. Arbukhanova1:100 (номер по каталогу ab5694), антититела к коллагену IV типа в разведении 1:200 (номер по каталогу ab6311), анти-коллаген VI в разведении 1:250 (номер по каталогу ab6588) (Abcam; Cambridge, UK). В качестве

(17) Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitroMatrix BiologyJanuary 1, 2020J.C. Ashworth et al.1:1000), pFAK (Tyr397, 44-624G Thermofisher, 1:100), collagen I (ab34710 AbCam, 1:100–1:500), collagen IV (ab6311 AbCam, 1:200), cleaved caspase 3 (9661 CST, 1:400) or CD44 (3570 CST, 1:400). After further washes in

(18) A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrierNature communicationsAugust 13, 2019Petrosyan A, Cravedi P, Villani V, Angeletti A, Manrique J, Renieri A, De Filippo RE, Perin L, Da Sacco S.1:100 Nephrin (NPHS1),Invitrogen,PA5-20330,WB: 1:1000 WT1,Abcam,ab15249,IF 1:50 "Col IV α1,2",Abcam,ab6311,IF 1:50 Col IV α3,Shigei Research Institute,H31,WB: 1:100 Col IV α4,Shigei Medical Research,RH42,IF:

(19) An Exosomal Urinary miRNA Signature for Early Diagnosis of Renal Fibrosis in Lupus NephritisCellsJuly 25, 2019Cristina Solé, Teresa Moliné, Marta Vidal, Josep Ordi-Ros, Josefina Cortés-Hernándezanti-SP1 (Abcam, ab124804), 1:100 mouse anti-COL1A1 (Abcam, ab6308), or 1:100 mouse anti-COL4A1 (Abcam, ab6311) overnight at 4 °C (more details in SI). 2.8. Human Kidney Cells Culture Primary human renal mesangial cells

(20) Using a Three-Dimensional Collagen Matrix to Deliver Respiratory Progenitor Cells to Decellularized Trachea In VivoTissue Engineering Part C: MethodsFebruary 1, 2019Nick J.I. Hamilton, Robert E. Hynds, Kate H.C. Gowers, Angela Tait, Colin R. Butler, Colin Hopper, Alan J. Burns, Martin A. Birchall, Mark Lowdell, Sam M. Janes5 [Abcam; ab17130], MUC5AC [Sigma-Aldrich; M5293], ACT [Sigma-Aldrich; T6793], collagen IV [Abcam; ab6311], fibronectin [Thermo Scientific; PA1-84601], laminin alpha 4 [Abcam; ab69634]) were applied to the

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Anti-Collagen IV antibody [COL-94] from Abcam

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Anti-Collagen IV antibody [COL-94] from Abcam

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