Anti-Nrf2 antibody ab89443 from Abcam

Product Specs
ItemAnti-Nrf2 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab89443
Conjugate/TagUnconjugated
ClonalityMonoclonal
HostMouse, Nude mouse
Antibody TypePrimary
IsotypeIgG2a
Quantity100 UG
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameNFE2L2
ReactivityHuman, Rat
TargetNrf2

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Abcam

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Microglia Sirt6 modulates the transcriptional activity of NRF2 to ameliorate high-fat diet-induced obesityMol MedAugust 15, 2023Xiaoxia Xiao, Huiling Hu, Yadi Zhong, Yingjian Chen, Kaijia Tang, Zhisen Pan, Jiawen Huang, Xiaoying Yang, Qi Wang, Yong GaoSubsequent western blot experiments were performed using Sirt6 (13572-1-AP, Proteintech) and NRF2 (ab89443, Abcam) antibodies. For the acetylation assays, the cells were lysed in lysis buffer containing a protease

(2) Treatment of radiation-induced brain injury with bisdemethoxycurcuminNeural regeneration researchFebruary 1, 2023Chang YQ, Zhou GJ, Wen HM, He DQ, Xu CL, Chen YR, Li YH, Chen SX, Xiao ZJ, Xie M.of mouse anti-GFAP (1:200, CST; Cat# 3670s, RRID: AB_561049) and mouse anti-Nrf2 (1:200, Abcam; Cat# ab89443, RRID: AB_2041334). After washing with PBS, the samples were incubated with Alexa Fluor 488 fluorescent

(3) Zearalenone Exposure Affects the Keap1–Nrf2 Signaling Pathway and Glucose Nutrient Absorption Related Genes of Porcine Jejunal Epithelial CellsToxins (Basel)November 14, 2022Qun Cheng, Shuzhen Jiang, Libo Huang, Yuxi Wang, Weiren Yangprocessing steps: washing with PBS three times for 5 min each, blocking with 10% FBS for 1 h, Nrf2 (1:500, ab89443, Abcam, Cambridge, UK), reactive oxygen species (ROS, 1:200, ab236409, Abcam, Cambridge, UK), Sglt1

(4) Dietary intake of deuterium oxide decreases cochlear metabolism and oxidative stress levels in a mouse model of age-related hearing lossRedox biologyNovember 1, 2022Hou S, Chen P, He J, Chen J, Zhang J, Mammano F, Yang J.M PBS, and then with a solution containing anti-nuclear factor erythroid 2-related factor 2 (Nrf2, #ab89443, Abcam, USA), anti-Heme-oxygenase 1 (HO-1, #ADI- SPA-896D Stressgen, Canada) or anti-glutathione (GSH,

(5) Nrf2 overexpression increases the resistance of acute myeloid leukemia to cytarabine by inhibiting replication factor C4Cancer gene therapyNovember 1, 2022Hu T, Pan C, Zhang T, Ni M, Wang W, Zhang S, Chen Y, Wang J, Fang Q.Multi Sciences, China. Matrigel Matrix was purchased from Corning Biocoat, China. Anti-Nrf2 antibody (#ab89443) came from Abcam (Cambridge, UK). The anti-RFC4 antibody was purchased from Gene Tex (#N1C3), USA, whereas

(6) ACE2 deficiency exacerbates obesity-related glomerulopathy through its role in regulating lipid metabolismCell Death DiscoverySeptember 30, 2022Yin-Yin Che, Han Hong, Yu-Ting Lei, Jia Zou, Yi-Ya Yang, Li-Yu He1:5000), FASN (Abcam, ab128870, 1:20,000 dilution), ACE2 (Abcam, ab108252, 1:3000 dilution), Nrf2 (Abcam, ab89443, 1:1000 dilution), SOD1 (Abcam, ab51254, 1:50,000 dilution), HO-1 (Abcam, ab52947, 1:2000 dilution),

(7) A major role for Nrf2 transcription factors in cell transformation by KSHV encoded oncogenesFrontiers in OncologySeptember 21, 2022Daiana Sapochnik, Ana R. Raimondi, Victoria Medina, Julian Naipauer, Enrique A. Mesri, Omar Cosocells. Western blot Nrf2 and phospho-Nrf2 were detected by Western blotting with anti-Nrf2 (Abcam ab89443) and anti-phospho-Nrf2 (Abcam ab76026). For kinase analysis, we used anti-ERK2 (Santa Cruz: sc-154),

(8) Biosynthesis of a water solubility-enhanced succinyl glucoside derivative of luteolin and its neuroprotective effectMicrobial biotechnologySeptember 1, 2022Chen L, Chang S, Zhao L, Li B, Zhang S, Yun C, Wu X, Meng J, Li G, Guo S, et al.in western blotting: rabbit anti‐HO‐1 (ab13243, 1:1000, from Abcam, Shanghai, China), mouse anti‐NRF2 (ab89443, 1:500, from Abcam), rabbit anti‐TUBULIN (10068‐1‐AP, 1:3000, from Proteintech, Shanghai, China), rabbit

(9) Exposure to airborne particulate matter induces renal tubular cell injury in vitro: the role of vitamin D signaling and renin-angiotensin systemHeliyonAugust 1, 2022Kang E, Yim HE, Nam YJ, Jeong SH, Kim JA, Lee JH, Son MH, Yoo KH.1:1000, PA5-20812; Thermo Fisher Scientific), nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1000, ab89443; Abcam), nuclear factor-kappa B p65 (NF-κB p65; 1:1000, sc-8008; Santa Cruz), tumor necrosis factor

(10) Dao-Chi Powder Ameliorates Pancreatitis-Induced Intestinal and Cardiac Injuries via Regulating the Nrf2-HO-1-HMGB1 Signaling Pathway in RatsFrontiers in PharmacologyJuly 11, 2022Jiaqi Yao et al.#A044) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The anti-Nrf2 (#ab89443), anti-HO-1 (#ab68477), and anti-HMGB1 (#ab79823) were purchased from Abcam (Cambridge, Massachusetts,

(11) The Sesquiterpene Lactone Cynaropicrin Manifests Strong Cytotoxicity in Glioblastoma Cells U-87 MG by Induction of Oxidative StressBiomedicinesJuly 2, 2022Rossella Rotondo, Maria Antonietta Oliva, Antonietta Arcella30 min. After blocking with 10% specific serum, the cells were incubated with antibody against Nrf2 (Ab89443, Abcam, Burkingane, CA, USA, 1:100) and Cyt c (BD Pharmingen™, San Diego, CA, USA, 1:100) overnight

(12) Pharmaceutical Activation of Nrf2 Accelerates Diabetic Wound Healing by Exosomes from Bone Marrow Mesenchymal Stem CellsInternational journal of stem cellsMay 30, 2022Wang L, Cai Y, Zhang Q, Zhang Y.Invitrogen), CD63 (1：5,000; PA5-100713; Invitrogen), TSG101 (1：1,000; ab133586; Abcam), Nrf2 (1：1,000; ab89443; Abcam), and β-actin (1：1,000; ab8226; Abcam), respectively, overnight at 4℃. The membranes were subsequently

(13) Oxymatrine inhibits the pyroptosis in rat insulinoma cells by affecting nuclear factor kappa B and nuclear factor (erythroid-derived 2)-like 2 protein/heme oxygenase-1 pathwaysThe Korean Journal of Physiology & PharmacologyMay 1, 2022Jingying Gao, Lixia Xia, Yuanyuan Weianti-IL-1β antibody (ab205924, 1:1,000), anti-NF-κB p65 antibody (ab16502, 1:2,000), anti-Nrf2 antibody (ab89443, 1:500), anti-HO-1 antibody (ab13243, 1:2,000), anti-Keap1 antibody (ab119403, 1:1,000), anti-ASC antibody

(14) Astragaloside-IV alleviates high glucose-induced ferroptosis in retinal pigment epithelial cells by disrupting the expression of miR-138-5p/Sirt1/Nrf2BioengineeredApril 1, 2022Xuyuan Tang, Xiuyi Li, Dongyan Zhang, Wei Hanadded onto the membrane, and primary antibodies (Sirt1, 1:1000, #ab189494, Abcam, UK; Nrf2, 1:1000, #ab89443, Abcam; LaminB, 1:1000, #ab16048, Abcam; GPX4, 1:1000, #ab51944, Abcam; GCLC, 1:2000, #ab207777, Abcam;

(15) Involvement of FoxO1, Sp1, and Nrf2 in Upregulation of Negative Regulator of ROS by 15d-PGJ2 Attenuates H2O2-Induced IL-6 Expression in Rat Brain AstrocytesNeurotoxicity ResearchFebruary 1, 2022Chen-Yu Wang, Chien-Chung Yang, Li-Der Hsiao, Chuen-Mao Yang(rabbit polyclonal antibody, Cat# ab37707, RRID:AB_1524434), Nrf2 (mouse monoclonal antibody, Cat# ab89443, RRID:AB_2041334), and phospho-Nrf2 (phospho-Ser40, Rabbit monoclonal antibody, Cat# ab76026, RRID:AB_1524049)

(16) Galangin Reverses H(2)O(2)-Induced Dermal Fibroblast Senescence via SIRT1-PGC-1α/Nrf2 SignalingInternational journal of molecular sciencesJanuary 26, 2022Lee JJ, Ng SC, Hsu JY, Liu H, Chen CJ, Huang CY, Kuo WW.(c-19) (sc-6298, Santa Cruz, CA, USA), anti-HO-1 (H-105) (sc-10 789, Santa Cruz, CA, USA), anti-Nrf2 (ab89443, Abcam, Cambridge, UK), anti-p-Nrf2 (S40) (ab76026, Abcam), anti-PGC-1α (D-5) (sc-518025, Santa Cruz,

(17) Paclitaxel-Induced Epidermal Alterations: An In Vitro Preclinical Assessment in Primary Keratinocytes and in a 3D Epidermis ModelInternational journal of molecular sciencesJanuary 20, 2022Montero P, Milara J, Pérez-Leal M, Estornut C, Roger I, Pérez-Fidalgo A, Sanz C, Cortijo J.and concentrations used were the following: SOD1 1:2000 (ab16831, Abcam, Cambridge, UK), Nrf2 1:1000 (ab89443, Abcam, Cambridge, UK), NOX4 1:1000 (NB100-58849, Novus Biologicals, Cambridge, UK), p53 1:1000 (ab131442,

(18) NRF2-directed PRPS1 upregulation to promote the progression and metastasis of melanomaFrontiers in immunologyJanuary 1, 2022Xiong G, Feng Y, Yi X, Zhang X, Li X, Yang L, Yi Z, Sai B, Yang Z, Zhang Q, et al.anti-N-Cadherin (Proteintech, 22018-1-AP), anti-Vimentin (Proteintech, 10366-1-AP), anti-NRF2 (Abcam, ab89443), anti-β-actin (Bioss, bs-0061R), and Tubulin (Abcam, #ab7291). The secondary antibodies used in the

(19) Nrf2 Overexpression Decreases Vincristine Chemotherapy Sensitivity Through the PI3K-AKT Pathway in Adult B-Cell Acute Lymphoblastic LeukemiaFrontiers in oncologyJanuary 1, 2022Wang L, Liu X, Kang Q, Pan C, Zhang T, Feng C, Chen L, Wei S, Wang J.streptomycin (100 mg/ml) at 37°C in a humidified atmosphere with 5% CO2. Reagents and Antibodies Anti-Nrf2 (ab89443) purchased from Abcam (Cambridge, UK). Anti-β-actin (20536-1-AP), anti-BAD (10435-1-AP), p-CREB (12208-1-AP),

(20) Heme Oxygenase-1 Protects Hair Cells From Gentamicin-Induced DeathFrontiers in cellular neuroscienceJanuary 1, 2022Yang Y, Chen X, Tian K, Tian C, Chen L, Mi W, Li Q, Qiu J, Lin Y, Zha D.anti-HO-1 (1:100, GTX101147, Gene Tex, Alton Pkwy Irvine, CA, United States), mouse anti-Nrf2 (1:200, ab89443, Abcam, Cambridge, MA, United States), goat anti-Sox2 (1:100, AF2018-SP, R&D Systems, Minneapolis, MN,

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(1)

3/3/2020Nrf2 Expression in Primary Murine Microglia Cells Lisha JoshiA quite good antibody for western blot.

Anti-Nrf2 antibody from Abcam

Abcam

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Anti-Nrf2 antibody from Abcam

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