Anti-KPNB1 antibody [3E9] ab2811 from Abcam

Product Specs
ItemAnti-KPNB1 antibody [3E9]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab2811
Antibody TypePrimary
ClonalityMonoclonal
HostMouse, Nude mouse
Quantity100 UL
IsotypeIgG2a
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Clone3000000000
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameKPNB1
ReactivityHuman, Mouse, Rat
TargetKPNB1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Visualizing the disordered nuclear transport machinery in situNatureMay 1, 2023Yu M, Heidari M, Mikhaleva S, Tan PS, Mingu S, Ruan H, Reinkemeier CD, Obarska-Kosinska A, Siggel M, Beck M, et al.buffer (3% BSA in PBS) for 90 min. The cells were subsequently immunolabelled with anti-KPNB1 antibody (ab2811, Abcam; at 1:1,000) and anti-mouse Alexa Fluor 488 secondary antibody (A-11001, Thermo Fisher; at 1:1,000).

(2) Therapeutic targeting of CNBP phase separation inhibits ribosome biogenesis and neuroblastoma progression via modulating SWI/SNF complex activityClinical and Translational MedicineApril 26, 2023Anpei Hu, Guo Chen, Banghe Bao, Yanhua Guo, Dan Li, Xiaojing Wang, Jianqun Wang, Qilan Li, Yi Zhou, Haiyang Gao, et al.49 , 52 , 53 using antibodies against CNBP (sc‐515387, Santa Cruz Biotechnology, Inc.), KPNB1 (ab2811), SMARCC2 (ab243634), Flag‐tag (ab125243, Abcam Inc.), Myc‐tag (#2276), His‐tag (#9991) or GST‐tag (#2624,

(3) HURP localization in metaphase is the result of a multi-step process requiring its phosphorylation at Ser627 residueFrontiers in cell and developmental biologyJanuary 1, 2023Didaskalou S, Efstathiou C, Galtsidis S, Kesisova I, Halavatyi A, Elmali T, Tsolou A, Girod A, Koffa M.Novus Biologicals, Littleton, CO, United States), anti-Importin ß mouse monoclonal antibody (1:1,000; ab2811, Abcam, Cambridge, United Kingdom), anti-GFP rabbit polyclonal antibody (1:1,000; ab290, Abcam, Cambridge,

(4) Mechanical force application to the nucleus regulates nucleocytoplasmic transportNature cell biologyJune 1, 2022Andreu I, Granero-Moya I, Chahare NR, Clein K, Molina-Jordán M, Beedle AEM, Elosegui-Artola A, Abenza JF, Rossetti L, Trepat X, et al.KPNA2 / Importin alpha 1 (MAB6207 Bio-techne, Clone number: 682239) 1:200, KPNB1 / Importin Beta 1 (ab2811 Abcam, RRID:AB_2133989) 1:200. The secondary antibodies used were Alexa Fluor 488 anti-mouse (A-11029;

(5) SARS-CoV-2 ORF6 disrupts nucleocytoplasmic trafficking to advance viral replicationCommunications biologyMay 19, 2022Miyamoto Y, Itoh Y, Suzuki T, Tanaka T, Sakai Y, Koido M, Hata C, Wang CX, Otani M, Moriishi K, et al.San Jose, CA, USA), HIF-1α (ab51608 [EP1215Y], Abcam), NF-κB p65 (#8242 [D14E12], CST), importin β1 (ab2811 [3E9], Abcam), CAS (ab96755, Abcam), Lamin A/C (sc-6215, Santa Cruz, Dallas, TX, USA), Flag (M2 [F1804],

(6) Cellular Stress Induces Nucleocytoplasmic Transport Deficits Independent of Stress GranulesBiomedicinesMay 3, 2022Joni Vanneste, Thomas Vercruysse, Steven Boeynaems, Philip Van Damme, Dirk Daelemans, Ludo Van Den Boschnumber: ab56574), anti-Yb1 (1/500, catalog number: ab76149) and anti-importin-β1 (1/1000, catalog number: ab2811) all provided by Abcam (Cambridge, UK). The cells were subsequently washed with DPBS and incubated with

(7) Karyopherin enrichment and compensation fortifies the nuclear pore complex against nucleocytoplasmic leakageJournal of Cell BiologyMarch 7, 2022Joanna Kalita, Larisa E. Kapinos, Tiantian Zheng, Chantal Rencurel, Anton Zilman, Roderick Y.H. Lim(11096176001; Roche) in TBS with Tween 20 (TBST) solution for at least 1 h at RT and probed for Kapβ1 [ab2811 (3E9); Abcam] or CRM1 (ABIN2778849; Antikörper). Simultaneously, membranes were probed for GAPDH (α-rabbit,

(8) H9N2 virus-derived M1 protein promotes H5N6 virus release in mammalian cells: Mechanism of avian influenza virus inter-species infection in humansPLoS pathogensDecember 1, 2021Li F, Liu J, Yang J, Sun H, Jiang Z, Wang C, Zhang X, Yu Y, Zhao C, Pu J, et al.from Beyotime. Anti-IAV NP antibody (ab20343) and anti-karyopherin subunit beta 1 antibody (KPNB1, ab2811) were from Abcam. Anti-junction plakoglobin antibody (JUP, 2309) was from CST, anti-cleft lip and palate

(9) Multiple pathways of toxicity induced by C9orf72 dipeptide repeat aggregates and G₄C₂ RNA in a cellular modeleLifeJune 23, 2021Frottin F, Pérez-Berlanga M, Hartl FU, Hipp MS.(1:1000) Antibody Rabbit polyclonal anti-KPNA4 Abcam Cat. #: ab84735 RRID:AB_1860702 IF (1:1000) Antibody Mouse monoclonal anti-KPNB1 Abcam Cat. #: ab2811 RRID:AB_2133989 IF (1:1000) Antibody Mouse monoclonal anti-GAPDH Millipore Cat. #: MAB374 RRID:AB_2107445 WB

(10) KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathwayCell death and differentiationApril 1, 2021Du W, Zhu J, Zeng Y, Liu T, Zhang Y, Cai T, Fu Y, Zhang W, Zhang R, Liu Z, et al.Inc., Chicago, IL, USA); anti-Bcl-2(sc-7382), anti-MerTK (sc-365499) (Santa Cruz, CA, USA); anti-KPNB1 (ab2811), anti-p-MerTK (Y749 + Y753 + Y754) (ab14921, Abcam, Cambridge, UK); anti-cyclinD1 (92G2), anti-AKT(11E7),

(11) Conformation of the nuclear pore in living cells is modulated by transport stateeLifeDecember 21, 2020Joan Pulupa, Harriet Prior, Daniel S Johnson, Sanford M Simonmonoclonal),Santa Cruz,sc-101540 RRID:AB_2133549,IF 1:500 Antibody,α-kap1ß/impß −1 (3E9) (mouse monoclonal),Abcam,ab2811 RRID:AB_2133989,IF 1:1000 Antibody,"anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa

(12) Recruitment of Host Nuclear Pore Components to the Vicinity of Theileria SchizontsmSphereFebruary 26, 2020Sandra Huber, Anina Bär, Selina Epp, Jacqueline Schmuckli-Maurer, Naja Eberhard, Bruno M. Humbel, Andrew Hemphill, Kerry Woodsrabbit anti-lamin B1 (ab16048, Abcam) (1:500 dilution), anti-importin B1 (KPNB1) clone 3E9 (Abcam, ab2811) (1:500), anti-alpha tubulin (clone DM1A; T9026, Sigma) (1:5000), anti-CLASP2 (KT69, Absea) (1:100),

(13) Cytoplasmic Parvovirus Capsids Recruit Importin Beta for Nuclear DeliveryJournal of VirologyJanuary 31, 2020Elina Mäntylä, Vesa Aho, Michael Kann, Maija Vihinen-Ranta(Thermo Fisher Scientiﬁc). In microinjection studies, an Imp ␤1 MAb (anti-KPNB1 [clone 3E9], catalogue number ab2811; Abcam, Cambridge, UK) was used to interfere with the docking of the Imp-substrate complex to the NPC. Confocal microscopy

(14) Karyopherin enrichment at the nuclear pore complex attenuates Ran permeabilityJournal of Cell ScienceJanuary 1, 2020Suncica Barbato, Larisa E. Kapinos, Chantal Rencurel, Roderick Y.H. LimScience SHORT REPORT were detected using the following primary antibodies (all Abcam): Kapβ1 (ab2811, 1:200), Kapα (ab6036, 1:200), RanGAP1 (ab2081, 1:500), RanGEF (ab54600, 1:200) and RanBP2 (ab64276,

(15) Mechanisms of nuclear content loading to exosomesScience AdvancesNovember 20, 2019Akira Yokoi et al.Santa Cruz Biotechnology) conjugated to Alexa 594, Lamin B1 (1:100; 8982, Abcam), and Importin (1:100; ab2811, Abcam) conjugated to Alexa 594. Conjugation of antibodies for Nesprin-2, LaminB1, and importin to Alexa

(16) Identification of candidate molecular targets of the novel antineoplastic antimitotic NP-10Scientific reportsNovember 14, 2019Yokoyama T, Yukuhiro M, Iwasaki Y, Tanaka C, Sankoda K, Fujiwara R, Shibuta A, Higashi T, Motoyama K, Arima H, et al.previously7. Antibodies used in this study were as follows: NUP155 (A303-934A, Bethyl Laboratories), IPOβ (ab2811, Abcam), KNTC1 (F2051-10H4, Sigma-Aldrich), hCAP-D2 (E4161-4C12, Sigma-Aldrich), IPO7 (ab88339, Abcam),

(17) Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-αNature communicationsSeptember 20, 2019Chang CC, Chen CJ, Grauffel C, Pien YC, Lim C, Tsai SY, Hsia KC.using antibodies against GM130 (Abcam ab52649), Importin-α (R&D Systems MAB6207), and Importin-β (Abcam ab2811). Uncropped scans of Western blots are provided in Supplementary Fig. 7. To pull down endogenous GM130,

(18) Phosphorylation of importin-α1 by CDK1-cyclin B1 controls mitotic spindle assemblyJournal of Cell ScienceSeptember 15, 2019Li Guo, Khamsah Suryati Mohd, He Ren, Guangwei Xin, Qing Jiang, Paul R. Clarke, Chuanmao Zhangfor WB; I1784; Sigma-Aldrich), mouse monoclonal anti-importin-β (1:100 for IF, 1:1000 for WB; 3E9; ab2811; Abcam), rabbit polyclonal anti-importin-β (raised against GST-tagged full-length importin-β and used

(19) Double-strand breaks in ribosomal RNA genes activate a distinct signaling and chromatin response to facilitate nucleolar restructuring and repairNucleic acids researchSeptember 5, 2019Korsholm LM, Gál Z, Lin L, Quevedo O, Ahmad DA, Dulina E, Luo Y, Bartek J, Larsen DH.#ab70369, 1:500), mouse alpha-tubulin (Santa Cruz, #sc-8035, 1:2000, IgM), mouse beta-importin (Abcam, #ab2811, 1:5000), mouse Mcm7 (Santa Cruz, DCS141, sc65469, 1:500), rabbit SMC1 (Abcam, #ab9262, 1:1000). RESULTS NBS1

(20) IGFBP-3 Induced by Ribotoxic Stress Traffics From the Endoplasmic Reticulum to the Nucleus in Mammary Epithelial CellsJournal of the Endocrine SocietyMarch 1, 2019Allyson Agostini-Dreyer, Amanda E Jetzt, Jennifer Skorupa, Jennifer Hanke, Wendie S Cohick(IP),AB_914704 [23] Importin-β,Full-length native bovine protein,Anti-NTF97/importin-β [3E9],Abcam ab2811,Mouse; mono,1:1000 (WB) 1:500 (IP),AB_2133989 [21] ,Mouse IgG,HRP horse anti-mouse IgG,Vector PI-2000,Horse,Varied,AB_2336177

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9/28/2016NTF97 antibody for Western Blotting Ganesh PusapatiThe hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this pathway results in basal cell carcinoma and medulloblastoma. A... Read Review

Anti-KPNB1 antibody [3E9] from Abcam

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Anti-KPNB1 antibody [3E9] from Abcam

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