Fig 2: Temporal profiling of the ERa interactome following treatment of MCF7 cells with OHT. a Volcano plots highlighting enriched or lost proteins in the ERa interactome upon OHT treatment for 2 h, 6 h and 24 h. b Boxplots illustrating the loss of ERa co-activator proteins CREBBP, NCOA3 and NRIP1 at 2 h and the enrichment of several subunits of the SWI/SNF and NuRD complexes at 6 h (left to right). Quantitative values are normalised so that the median of the vehicle treated samples is zero (centered around the median of vehicle). Centre line shows the median, bounds of box correspond to the first and third quartiles and the upper and lower whiskers extend to the largest or the smallest value no further than 1.5 × IQR (inter-quartile range)

Fig 3: The LANA SET homology sequence interacts with p53 and acetylation reduces LANA–p53 interaction. (A) Schematic of LANA with acidic SET homology regions indicated below. Sequence alignments were determined with LALIGN. Identity and similarity are shown. (B) GFP LANA 275–435 interacts with p53. GFP, GFP-LANA, or GFP-LANA 275–435 and Flag-p53 were cotransfected into 293T cells. At 48 h after transfection, Flag p53 was immunoprecipitated with M2-Flag Ab, and Western blot analysis was performed. (C) Expression of CBP, which acetylates p53, reduces the LANA–p53 interaction. pT7LANA, Flag-p53, and HA-CBP were transfected into 293T cells. At 48 h after transfection, Flag p53 was immunoprecipitated using M2-Flag Ab, and Western blot analysis was performed. Data in B and C are representative of at least three independent experiments.