Fig 1: Histological images of papillomas and squamous cell carcinomas (SCCs) of Dog 1 under hematoxylin and eosin staining (H&E) and immunohistochemistry staining (IHC). (a) Multiple masses taken from the trunk and digits were diagnosed as papillomas using H&E (40 ×). (b) The masses taken from the inguinal region were diagnosed as squamous cell carcinomas (SCCs) with a stromal invasive pattern, moderate keratinization, and nuclear pleomorphism under H&E (100 ×). (c) Antigen-specific IHC was performed using anti-HPV antibodies (BPV-1/1H8 + CAMVIR; catalog no. ab2417; Abcam, Cambridge, UK). Scattered positive signals were observed (400 ×).
Fig 2: Histological images of a viral papilloma of Dog 2 under hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) staining. The benign viral papilloma was taken from a cutaneous mass growing on the abdomen. (a) Hyperpigmented and proliferative epithelia were observed under H&E (40 ×). (b) Several eosinophilic intra-nuclear inclusion bodies (labeled by arrows) were identified in the stratum granulosum (200 ×). (c) Antigen-specific IHC was performed using anti-HPV antibodies (BPV-1/1H8 + CAMVIR; catalog no. ab2417; Abcam, Cambridge, UK). Positive signals (labeled by arrows) were observed on the viral inclusions (400 ×).
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