alamarBlue® from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemalamarBlue®
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberBUF012A
FormatReagent
Quantity25 ml
ApplicationsELISA,Functional Assays,Immunofluorescence
TypeAccessory Reagent

Add to Compare List

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Screen for New Antimicrobial Natural Products from the NCI Program for Natural Product Discovery Prefractionated Extract LibraryACS Infectious DiseasesJune 9, 2023Lucero Martínez-Fructuoso, S. J. Ryan Arends, Vitor F. Freire, Jason R. Evans, Sean DeVries, Brian D. Peyser, Rhone K. Akee, Christopher C. Thornburg, Rohitesh Kumar, Susan Ensel, et al.Growth of the 4 strains was monitored using the metabolic indicator dye, alamarBlue (BioRad; catalogue #BUF012B). After plates were incubated overnight, 5 μL of alamarBlue reagent (i.e., ∼10% final volume) was added

(2) Alleviating iatrogenic effects of paclitaxel via anti-inflammatory treatmentResearch SquareMay 26, 2023Mengwei Zhang, Saran Lotfollahzadeh, Nagla Elzinad, Xiaosheng Yang, Murad Elsadawi, Adam Gower, Mostafa Belghasem, Tarek Shazly, Vijaya B. Kolachalama, Vipul ChitaliaCell viability assay ECs seeded in 96-well plates underwent survival assay using alamarBlue solution (Cat No. BUF012B; Bio-Rad) per the manufacturer’s instructions. RNA extraction ECs were seeded in p60 plates, treated

(3) Optimized metrics for orthogonal combinatorial CRISPR screensScientific ReportsMay 6, 2023Ronay Cetin, Martin Wegner, Leah Luwisch, Sarada Saud, Tatjana Achmedov, Sebastian Süsser, Antonella Vera-Guapi, Konstantin Müller, Yves Matthess, Eva Quandt, et al.DMSO or Nutlin-3 (Selleckchem, S8059) (1 µM). Cell viability was recorded with alamarBlue (BioRad, BUF012A) according to the manufacturer's protocol for 14 days. gRNA sequences for the Cas activity experiment

(4) Utilizing mixture design response surface methodology to determine effective combinations of plant derived compounds as prostate cancer treatmentsCancer Rep (Hoboken)April 1, 2023Ian Geddes Berlin, Charity Conlin Jennings, Spencer Shin, Jason Kenealeywhich the other treatments were normalized to. After 48 h of incubation, 10 μL of AlamarBlue (BioRad, BUF012A) was added to each well containing cells and placed in the incubator for an additional 5–6 h. A time

(5) Dantrolene inhibits lysophosphatidylcholine-induced valve interstitial cell calcific nodule formation via blockade of the ryanodine receptorFrontiers in Cardiovascular MedicineMarch 30, 2023Christopher B. Sylvester, Farshad Amirkhosravi, Angelina S. Bortoletto, William J. West, Jennifer P. Connell, K. Jane Grande-Allendetermine the appropriate treatment concentration of dantrolene, we first used an alamarBlue (Bio-Rad BUF012) cell viability assay (19). Cells were treated with serial dilutions of dantrolene overnight in an incubator.

(6) Alamar Blue assay optimization to minimize drug interference and inter-assay viabilitybioRxivMarch 23, 2023Mina N. Dinh, Masahiro Hitomi, Zahraa A. Al-Turaihi, Jacob G. Scott(Cat# 10483, and lapatinib (Cat# 11493) were obtained CaymanChemical (Ann Arbor, MI). Alamar Blue(Cat# BUF012A) was purchased from Bio-Rad (Hercles, CA). Sodium dodecyl sulfate (Cat# 28312) was ordered from ThermoFisher

(7) Concurrent silencing of TBCE and drug delivery to overcome platinum-based resistance in liver cancerActa Pharmaceutica Sinica BMarch 1, 2023Senlin Li, Siyu Chen, Zhihui Dong, Xingdong Song, Xiuling Li, Ziqi Huang, Huiru Li, Linzhuo Huang, Ganyuan Zhuang, Ran Lan, et al.NP(siTBCE+DDP) were added into the fresh medium. Cell viability was measured by AlamarBlue reagent (BIO-RAD, BUF012A, California, America) at predetermined intervals. 2.10 Colony formation assay Cells were grown in

(8) Anticancer Activities of Novel Nicotinamide Phosphoribosyltransferase Inhibitors in Hematological MalignanciesMoleculesFebruary 16, 2023Paulina Biniecka, Saki Matsumoto, Axel Belotti, Jessie Joussot, Jian Fei Bai, Somi Reddy Majjigapu, Paul Thoueille, Dany Spaggiari, Vincent Desfontaine, Francesco Piacente, et al.Proliferation Determination The cell proliferation was assessed with alamarBlue® reagent (Bio-Rad, BUF012B, Hercules, CA, USA), which is based on REDOX reaction by viable cells. Specifically, resazurin sodium

(9) The Role of Acetyl Zingerone and Its Derivatives in Inhibiting UV-Induced, Incident, and Delayed Cyclobutane Pyrimidine DimersAntioxidants (Basel)January 26, 2023Jyoti Srivastava, Montana M. Young, Vipin Kumar Yadav, Pravin R. Phadatare, Thomas A. Meyer, Ratan K. Chaudhuri, Sanjay Premisurvival was assessed using the oxidation of Resazurin to the fluorescent Resorufin (Alamar blue dye, Cat. BUF012A, Bio-Rad, Hercules, CA, USA) following manufacturer’s instructions and published protocols [29]. In

(10) Enhanced genomic stability of new miRNA-regulated oncolytic coxsackievirus B3Molecular Therapy - OncolyticsDecember 15, 2022Huitao Liu, Amirhossein Bahreyni, Yasir Mohamud, Yuan Chao Xue, William W.G. Jia, Honglin LuoCell viability assay Cell viability was evaluated using alamarBlue according to the manufacture’s protocol (#BUF012A, Bio-Rad Laboratories). Briefly, the alamarBlue solution was added to the 48-well plate to a final concentration

(11) SARS-CoV-2 Spike Proteins and Cell-Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy PathogenesisInternational journal of molecular sciencesSeptember 9, 2022Bhargavan B, Kanmogne GD.S-proteins, rTFPI, DTNB, and BDAC3 was assessed by AlamarBlue assay, using the AlamarBlue reagent (cat# BUF012, Bio-Rad, Hercules, CA, USA), in accordance with the manufacturer’s instructions. Doses shown not to

(12) NAD(+) metabolism drives astrocyte proinflammatory reprogramming in central nervous system autoimmunityProceedings of the National Academy of Sciences of the United States of AmericaAugust 30, 2022Meyer T, Shimon D, Youssef S, Yankovitz G, Tessler A, Chernobylsky T, Gaoni-Yogev A, Perelroizen R, Budick-Harmelin N, Steinman L, et al.readings were then normalized to viable cell numbers as determined by the AlamarBlue assay (Bio-Rad, #BUF012B). Normalized values are presented as CD38 activity. Isolation and FACS Analysis of Adult Mice CNS Cells. Naïve

(13) Optimization of the Rheological Properties of Self-Assembled Tripeptide/Alginate/Cellulose Hydrogels for 3D PrintingPolymers (Basel)May 30, 2022Alejandro Hernández-Sosa, Rosa Ana Ramírez-Jiménez, Luis Rojo, Fouzia Boulmedais, María Rosa Aguilar, Miryam Criado-Gonzalez, Rebeca Hernández(n = 8). After 24 h of incubation, the media was removed and 100 µL of the Alamar Blue (AB, BIO-RAD BUF012B) solution prepared in warm DMEM–low glucose phenol red-free (10% v/v) (Gibco) was added to each well

(14) Targeting farnesylation as a novel therapeutic approach in HRAS-mutant rhabdomyosarcomaOncogeneMay 1, 2022Odeniyide P, Yohe ME, Pollard K, Vaseva AV, Calizo A, Zhang L, Rodriguez FJ, Gross JM, Allen AN, Wan X, et al.(control) or a range of doses of tipifarnib. 96 h later, medium was removed and alamarBlue (BioRad cat # BUF012B) cell viability assay reagent was added at 1:10 ratio in culture media. After 4 h incubation, fluorescence

(15) Ventilation‐induced epithelial injury drives biological onset of lung trauma in vitro and is mitigated with prophylactic anti‐inflammatory therapeuticsBioengineering & Translational MedicineMay 1, 2022Eliram Nof, Arbel Artzy‐Schnirman, Saurabh Bhardwaj, Hadas Sabatan, Dan Waisman, Ori Hochwald, Maayan Gruber, Liron Borenstein‐Levin, Josué Sznitmanab14084, Abcam). Metabolic activity tests were performed using an alamarBlue cell viability assay (BUF012, Bio‐rad). The absorbance of the incubated media containing 10% alamarBlue (2 h) was measured at 570

(16) Insulin enhances neurite extension and myelination of diabetic neuropathy neuronsThe Korean Journal of PainApril 1, 2022Vuong M. Pham, Nitish Thakorassay The proliferation rate of SCs under hyperglycemic culture was evaluated by alamarBlue® (AB) (BUF012B, Bio-Rad, Hercules, CA) staining at 1, 3, 5, and 7 days of culture. SCs were seeded into a 96-well plate

(17) Negative Immune Checkpoint Protein, VISTA, Regulates the CD4+ Treg Population During Sepsis Progression to Promote Acute Sepsis Recovery and SurvivalFrontiers in ImmunologyMarch 24, 2022Chyna C. Gray, Bethany Biron-Girard, Michelle E. Wakeley, Chun-Shiang Chung, Yaping Chen, Yael Quiles-Ramirez, Jessica D. Tolbert, Alfred AyalaAB_1107773) for 30 min at 37°C, 5% CO2 then stained with alamarBlue (Bio-Rad, Hercules, CA, USA: product code BUF012A) according to the manufacturer’s protocol. Sample absorbance was measured every 24 h for 7 days using

(18) Prediction of histone post-translational modification patterns based on nascent transcription dataNature GeneticsMarch 10, 2022Zhong Wang et al.Triptolide alone or a Triptolide + Trichostatin A dual treatment, then incubated with almarBlue (BIORAD, BUF012A) following the BIORAD protocol. Absorbance of cells incubated with almarBlue was measured at 590 nm.

(19) Surface Functionalization of Ureteral Stents-Based Polyurethane: Engineering Antibacterial CoatingsMaterials (Basel)February 23, 2022Kardelen Ecevit, Eduardo Silva, Luísa C. Rodrigues, Ivo Aroso, Alexandre A. Barros, Joana M. Silva, Rui L. Reisa 96-well polystyrene flat-bottom, and 200 μL of TSB media including 10%(v/v) AB dye (Bio-Rad, ref BUF012, Kidlington Oxford, UK) was added. Besides, it should be noted TSB media, including 10%(v/v) AB, was

(20) PEGylated talazoparib enhances therapeutic window of its combination with temozolomide in Ewing sarcomaiScienceFebruary 18, 2022Vanessa Del Pozo, Andrew J. Robles, Shaun D. Fontaine, Qianqian Liu, Joel E. Michalek, Peter J. Houghton, Raushan T. Kurmashevaassays,Critical commercial assays,Critical commercial assays ,, Alamar Blue,Bio-Rad Laboratories,Cat#BUF012B ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, A-673

Be the First to Write a Review!

alamarBlue®

alamarBlue®

alamarBlue®

alamarBlue® from Bio-Rad (Formerly AbD Serotec)

Description

Bio-Rad

Citations (112)

Reviews

alamarBlue® from Bio-Rad (Formerly AbD Serotec)

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map