CCF4-AM is a lipophilic, esterified form of the CCF4 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF4-AM into its negatively charged form, CCF4, which is retained in the cytosol. CCF4 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for beta-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer® technology.
- Life Technologies
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- 20 mg
- Beta-Lactamase Activity Assays:Cell Analysis:Cell-Based β-Lactamase Assays:Cell-Based GPCR Reporter Assays:Cell-Based Nuclear Receptor Reporter Assays:Cell-Based Reporter Assays:Cellular Pathway Analysis Assays:Drug Discovery & Development:Enzyme & Protein Activity Assays:G-Protein Coupled Receptor Biology:Kinase Biology:Kinase Pathway Analysis:Nuclear Receptor Biology:Other Cellular Pathway Assay Products:Pathway Biology:Proteins, Expression, Isolation & Analysis:Reporter Assays:Target & Lead Identification & Validation:Cellular Imaging & Microscopy