Original MessageFrom:Yvonne18Date:03/28/2013 04:50:00In response to your message from 3/28/2013 2:30:00 AMHere is the details of Caco-2 cell line (Seed Details:- CACO-2, Accession No 86010202, Lot No. 10J006, Pass 42, date 01/11/2010)If culturing from a cry-frozen batch of cells, take care to thaw the cells quickly, and immediately adding them to 10ml of fetal bovine serum and spinning them down (5min at 1500rpm). For the culture medium you could try adding HEPES buffer (25mM) to keep the pH stable and some protocols also prescribe adding human transferrin to the culture medium. Products like "glutaMax" could also help to stabilise the cell attachment and growth.Hope this helps,Best regards,Leonie Vogt
Original MessageFrom:luo142Date:10/08/2012 13:54:00Thank you so much. I have another concern: I am also wondering that the HBSS is commonly used for cellular uptake of caco-2 cells, but I assume that it should be Ok to use DMEM, too? Could you please share your opinion, or some reference on this? Thank you so much!
Dear BlueDevil,Yes I agree. I will look into it more :)Many thanks!Regards,Kam
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