serotyping E. coli
I have a problem with serotyping of E. coli.
I am rather new in serotyping of these bacteria (except O157).
The problem is that I receive a positive reaction to a group antiserum (for several antibodies),
but when I try with specific O antisera, there is no positive reaction for none antisera in this group.
I have read some protocols about boiled cultures (to remove capsule), but this has yealded with no result at all.
I wonder what am I doing wrong.
The type of antisera for E. coli are OK antisera for screening of surface antigens on live bacterial cultures. Can I use these type of antisera for boiled cultures?
I would appreciate any help.
Apparently, antibody-based serotyping "is so tedious that its use is restricted to a few reference laboratories
". That quote is taken from the article at this link
, which describes the construction of a microarray for E. coli serotyping. This article link
describes a similar procedure. Depending on the amount of serotyping you will be doing, a microarray approach might be the way to go. It's at least worth reading over the literature.
If you have trouble accessing the articles, post your email address and I will try to send pdfs.