gateway pDEST vectors

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while
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gateway pDEST vectors
3/2/2012 7:17:00 AM
I didn't know, that the pDEST vectors contain any lacO sequence,
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gateway pDEST vectors
2/29/2012 10:02:00 AM
The IPTG is a lactose mimic and allows transcription of downstream elements.
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BlueDevil
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gateway pDEST vectors
1/30/2012 2:35:00 PM
Thank you for the reply!
I didn't know, that the pDEST vectors contain any lacO sequence, so without IPTG there is only some basal transcription or no transcription at all?


There can be a little leakiness to the system so it's possible to see some protein being made in the absence of IPTG. Overall, it's pretty good. The addition of IPTG at an optimal concentration (which should be determined by titration for your specific application) should result in a very large increase in protein expression.
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nadine.schweizer...
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gateway pDEST vectors
1/27/2012 10:34:00 PM
Thank you for the reply!
I didn't know, that the pDEST vectors contain any lacO sequence, so without IPTG there is only some basal transcription or no transcription at all?
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BlueDevil
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gateway pDEST vectors
1/27/2012 5:14:00 PM
Hi,
I want to express my protein from the pDEST15 and pDEST17 vectors, what I am puzzeled about is, that in the information for these vectors, it says inducible by IPTG.
Doesn't it only depend on the expression cells what kind of inducer I need to take, like arabinose or IPTG e.g.?


It just depends on the vector. The pDEST vectors, and many others, contain activation elements borrowed from the lac operon. The IPTG is a lactose mimic and allows transcription of downstream elements.
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nadine.schweizer...
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gateway pDEST vectors
1/25/2012 8:24:00 PM
Hi,
I want to express my protein from the pDEST15 and pDEST17 vectors, what I am puzzeled about is, that in the information for these vectors, it says inducible by IPTG.
Doesn't it only depend on the expression cells what kind of inducer I need to take, like arabinose or IPTG e.g.?
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