Hi,
I want to express my protein from the pDEST15 and pDEST17 vectors, what I am puzzeled about is, that in the information for these vectors, it says inducible by IPTG.
Doesn't it only depend on the expression cells what kind of inducer I need to take, like arabinose or IPTG e.g.?
It just depends on the vector. The pDEST vectors, and many others, contain activation elements borrowed from the lac operon. The IPTG is a lactose mimic and allows transcription of downstream elements.