Description
Using a novel approach to target enrichment, the NEBNext Direct™ Cancer Hotspot Panel enables highly specific hybridization-based capture of 190 common cancer targets from 50 genes. The NEBNext Direct technology offers significant advantages over both traditional in-solution hybridization and multiplex PCR protocols. Target enrichment is combined with library preparation, reducing processing time and minimizing sample loss. Ideal for automation, NEBNext Direct enables deep sequencing of genomic regions of interest for the discovery and identification of low frequency variants from challenging sample types.
Features: - Generate a higher percentage of your sequencing reads aligning to your targets
- Eliminate the need to over-sequence, reducing cost per sample
- Obtain uniform sequencing of all targets, regardless of GC content
- Save time with a 1-day workflow that combines enrichment with library preparation
- Generate high quality libraries with limited input amounts and degraded DNA samples, including FFPE and ctDNA
- Distinguish molecular duplicates, reducing false positive variants and improving sensitivity