NEBNextLibrary Quant Kit for Illumina from New England Biolabs

The NEBNext Library Quant Kit delivers significant improvements to qPCR-based library quantitation for next gen sequencing. The NEBNext Library Quant Kit for Illumina contains components that are optimized for qPCR-based quantitation of libraries prepared for Illumina next-generation sequencing platforms. The NEBNext Library Quant Kit contains primers which target the P5 and P7 Illumina adaptor sequences and a set of high-quality, pre-diluted DNA standards to enable reliable quantitation of diluted DNA libraries between 150–1000 bp.

Features:

• Provides more accurate and reproducible quant values than alternative methods and kits
• Compatible with libraries with a broad range of insert sizes and GC content, made by a variety of methods
• Requires only 4 standards, allowing more libraries to be quantitated per kit
• Supplied with a convenient, Library Dilution Buffer
• The NEBNext Library Quant Master Mix requires only the addition of primers
• Utilizes a single extension time for all libraries, regardless of insert size
• Library quant values can be easily calculated using NEB’s online tool
• ROX is included in the kit, for use with qPCR instruments that require a reference dye for normalization