BD FACSMelody™ Cell Sorter from BD Biosciences

Product Specs
ItemBD FACSMelody™ Cell Sorter
CompanyBD Biosciences
Price Get Pricing Get Quote for this Product
Catalog NumberBD FACSMelody
Dimensions(WxDxH) Cell Sorter: 49.5 x 55.9 x 48.3 cm; Electronics Box: 50.8 x 55.9 x 48.3 cm
QuantityEA
ApplicationsImmunology, genomics, cell and cancer biology, life sciences, stem cell, bioprocessing
DetectionFSC, SSC, Up to 9 fluorescence detection channels with optional changeable filters
Absorbance / Excitation488 nm (Blue), 640 nm (Red), 405 nm (Violet), 561 nm (Yellow/Green)
Purity> 98%
Sample Type5.0-mL polystyrene and polypropylene tubes
Sensitivity30 molecules of equivalent soluble fluorochrome PE (MESF-PE), 80 MESF-FITC (lower numbers indicate higher system sensitivity)
Yield> 80%
Throughput10,000 cells/second; 40,000 events per second; 2-way sorting
Analysis TimeInquire
ResolutionFull peak coefficient of variation (FPCV) of < 3.0%
Sample VolumeInput: 5 mL tubes Two-way sorting: 1.5-, 2.0- and 5.0-mL tubes One-way sorting: 6-, 24-, 48-, 96- and 384-well plates, 96-well PCR tray, microscope slide (optional)
Flow Rate34,000 drops/second

Add to Compare List

BD Biosciences

2350 Qume Drive

San Jose, California 95131

United States

Company Profile

Website: http://www.bdbiosciences.com/

(1) STIM-mediated calcium influx regulates maintenance and selection of germinal center B cellsJournal of Experimental MedicineJanuary 1, 2024Yutaro Yada, Masanori Matsumoto, Takeshi Inoue, Akemi Baba, Ryota Higuchi, Chie Kawai, Masashi Yanagisawa, Daisuke Kitamura, Shouichi Ohga, Tomohiro Kurosaki, et al.isolation of B cells and adoptive transfer Cell sorting was done on BD FACS Aria II (RRID:SCR_018091) or BD FACSMelody Cell Sorter (RRID:SCR_023209). For isolation of single IgG1+NIP+CD38lowB220+ clones, splenocytes of BM chimeric

(2) Obesity-induced PARIS (ZNF746) accumulation in adipose progenitor cells leads to attenuated mitochondrial biogenesis and impaired adipogenesisSci RepDecember 27, 2023Kazuki Hachiya, Yusuke Deguchi, Takuro Hirata, Tomoya Arikawa, Hiroto Fukai, Tatsuhiro Esashi, Kota Nagasawa, Yuhei Mizunoe, Yuka Nozaki, Masaki Kobayashi, et al.cell strainer (BD Biosciences) and resuspended in 2% FBS. Cells were analyzed with a flow cytometer (BD FACSMelody; BD Biosciences). Quantification of mitochondrial membrane potential Cells plated on a glass-bottom

(3) Heart Failure Promotes Cancer Progression in an Integrin β1-Dependent MannerInt J Mol SciDecember 11, 2023Irina Langier Goncalves, Lama Awwad, Sharon Aviram, Talel Izraeli, Laris Achlaug, Ami Aronheim48 h thereafter for GFP sorting. Single-cell clones of GFP positive were isolated by a cell sorter (BD FACSMelody, BD Biosciences, Franklin Lakes, NJ, USA; FacsChorus version 1.4.3.0) in 96-well plates (doublet discriminated;

(4) Co-encapsulated astaxanthin and kaempferol nanoparticles: fabrication, characterization, and their potential synergistic effects on treating non-alcoholic fatty liver disease†RSC AdvNovember 30, 2023Ho Thi Oanh, Ngo Thi Hoai Thu, Nguyen Van Hanh, Mai Ha Hoang, Hoang Thi Minh Hienaccordance with the procedure outlined in Hien et al.17 The stained cells were identified utilizing the BD FACSMelody™ Cell Sorter (BD Biosciences, San Diego, CA, USA) and subsequently analyzed employing the BD FACSChorus software

(5) The Parkinson’s drug entacapone disrupts gut microbiome homeostasis via iron sequestrationbioRxivNovember 16, 2023Fátima C. Pereira, Xiaowei Ge, Jannie Munk Kristensen, Rasmus H. Kirkegaard, Klara Maritsch, Yifan Zhu, Marie Decorte, Bela Hausmann, David Berry, Kenneth Wasmund, et al.The flow cytometry analysis of the microbial cells present in the suspension was performed using a BD FACSMelody™ (BD Biosciences), equipped with a BD FACSChorus™ software (BD). Briefly, background signals from the

(6) Characterization of neural stem cells derived from human stem cells from the apical papilla undergoing three-dimensional neurosphere inductionJournal of Applied Oral ScienceNovember 13, 2023Anupong Thongklam SONGSAAD, Sarut THAIRAT, Peeratchai SEEMAUNG, Amarin THONGSUK, Tatcha BALIT, Nisarat RUANGSAWASDI, Chareerut PHRUKSANIYOM, Thanasup GONMANEE, Kenneth L. WHITE, Charoensri THONABULSOMBATanalyzed to assess the presence and profile of antigen molecules. This analysis was conducted with the BD FACSMelody™ Cell sorter (BD Biosciences, San Jose, CA, USA). Five specific antibodies were used as markers for MSCs properties,

(7) PAR4 Inhibition Reduces Coronary Artery Atherosclerosis and Myocardial Fibrosis in SR-B1/LDLR Double Knockout MiceArteriosclerosis, Thrombosis, and Vascular BiologyNovember 1, 2023Samuel K. Lee, Rida A. Malik, Ji Zhou, Wei Wang, Peter L. Gross, Jeffrey I. Weitz, Rithwik Ramachandran, Bernardo L. Trigattiaddition of 400 µL PBS containing 1% formaldehyde. Data for 10 000 gated events were collected using a BD FACSMelody flow cytometer, and analysis was performed using BD FACSChorus and FlowJo 10.6.2 software. Histology For

(8) Human CD79b+ neutrophils in the blood are associated with early-stage melanomaFrontiers in ImmunologyOctober 31, 2023Melissa A. Meyer, Huy Q. Dinh, Ahmad Alimadadi, Daniel J. Araujo, Nandini Chatterjee, Norma A. Gutierrez, Yanfang Peipei Zhu, Emma L. Hunter, Shu Liang, Gregory Seumois, et al.dark. Cells were then washed with FACS buffer and filtered through 70μm strainers. Cells were sorted on BD FACSMelody or BD FACSARIA, received into TriZOL LS Reagent (ThermoFisher Scientific), and frozen immediately at

(9) Mouse methylation profiles for leukocyte cell types, and estimation of leukocyte fractions in inflamed gastrointestinal DNA samplesPLOS ONEOctober 5, 2023Kazuhiro Nishiyama, Hitomi Nishinakamura, Hideyuki Takeshima, Liu Yuyu, Chihiro Takeuchi, Naoko Hattori, Haruna Takeda, Satoshi Yamashita, Mika Wakabayashi, Kotomi Sato, et al.positive selection on a MACS column. Purity of the isolated specific leukocytes was evaluated using a BD FACSMelody cell sorter (BD Biosciences) with specific antibodies [dendritic cells (CD3- CD11c+ I-Ab+), monocytes (CD3- CD11b+),

(10) Identification of a non-canonical ciliate nuclear genetic code where UAA and UAG code for different amino acidsPLOS GeneticsOctober 5, 2023Jamie McGowan, Estelle S. Kilias, Elisabet Alacid, James Lipscombe, Benjamin H. Jenkins, Karim Gharbi, Gemy G. Kaithakottil, Iain C. Macaulay, Seanna McTaggart, Sally D. Warring, et al.(5–50 cells) were sorted into a 384-well plate containing 5 μL of autoclaved source water using FACS (BD FACSMelody Cell Sorter, BD Biosciences). 10 μL of RLT+ lysis buffer (Qiagen) was then added to each well and the plate was

(11) Comparison of Two Human Skin Cell Isolation Protocols and Their Influence on Keratinocyte and Fibroblast CultureInternational Journal of Molecular SciencesSeptember 28, 2023Álvaro Sierra-Sánchez, Martin A. Barbier, Brice Magne, Danielle Larouche, Salvador Arias-Santiago, Lucie Germainin 200 μL of PBS FACS solution (PBS 1X, 0.5 mM EDTA (Sigma-Aldrich) and 2% Serum) and analyzed using BD FACSMelody™ Cell Sorter (BD Biosciences). 4.6. Statistical Analysis Microsoft Excel software (v2308) was used for primary

(12) An inducible germ cell ablation chicken model for high-grade germline chimerasDevelopment (Cambridge, England)September 15, 2023Chen YC, Saito D, Suzuki T, Takemoto T.analysis For fluorescent cell enrichment sorting and analysis, experiments were conducted using a BD FACSMelody cell sorter (BD Biosciences) with the default configuration and equipped with blue (488 nm), red (640 nm), yellow-green

(13) Intrathecal CD8+CD20+ T Cells in Primary Progressive Multiple SclerosisNeurology - Neuroimmunology NeuroinflammationSeptember 1, 2023Marina Rode von Essen, Jacob Talbot, Rikke Holm Holm Hansen, Helene Højsgaard Chow, Henrik Lundell, Hartwig Roman Siebner, Finn Sellebjerg(BV605; IP26) and CD20 (PE/Cy7; 2H7) both from BioLegend and sorted into CD20− and CD20+ T cells on a BD FACSMelody BRV 9 color (BD) by fluorescence-activated cell sorting. The purity of CD20− T cells was 100% and 98%–100%

(14) MKL-1 suppresses ferroptosis by activating system Xc- and increasing glutathione synthesisInternational Journal of Biological SciencesAugust 21, 2023Zhou-Tong Dai, Yong-Lin Wu, Xing-Rui Li, Xing-Hua Liaoand incubated at 37°C for 20 minutes. After centrifugation, PBS was used to resuspend the cells. The BD FACSMelody flow cytometer (BD, USA) was used to analyze the samples for lipid ROS production. 2.10 GSH assay The

(15) Modifications of The Human Liver Cancer Cells through microRNA-145-Mediated Targeting of CDCA3Cell JAugust 20, 2023Yongqiang Lai, Junhao Liu, Xiao Hu, Xiancheng Zeng, Peng Gaofor 30 minutes at 37°C. The cells were then subjected to cell cycle analysis using flow cytometry (BD FACSMelody™ 4-Way Cell Sorter, BD, Franklin Lakes, NJ, USA). The experiment was repeated thrice. Analysis of migration

(16) Comparative transcriptomics of porcine liver-resident CD8αdim, liver CD8αhigh and circulating blood CD8αhigh NK cells reveals an intermediate phenotype of liver CD8αhigh NK cellsFrontiers in ImmunologyAugust 18, 2023Leen Hermans, Sofie Denaeghel, Robert J. J. Jansens, Steffi De Pelsmaeker, Filip Van Nieuwerburgh, Dieter Deforce, Everardo Hegewisch-Solloa, Emily M. Mace, Eric Cox, Bert Devriendt, et al.Bergisch Gladbach, Germany) followed by fluorescence-activated cell sorting (FACS) purification using a BD FACSMelody Cell Sorter (BD Biosciences, Franklin Lakes, NJ, USA) and monoclonal antibodies against porcine CD172a (IgG1, clone

(17) Defining cardiac functional recovery in end-stage heart failure at single-cell resolutionNature Cardiovascular ResearchAugust 16, 2023Junedh M. Amrute, Lulu Lai, Pan Ma, Andrew L. Koenig, Kenji Kamimoto, Andrea Bredemeyer, Thirupura S. Shankar, Christoph Kuppe, Farid F. Kadyrov, Linda J. Schulte, et al.incubate for 5 minutes before sorting. DRAQ5+ nuclei were sorted into 300 μl of nuclei wash buffer using a BD FACSMelody (BD Biosciences) with a 100 μM nozzle. Sorted nuclei were then centrifuged using the above conditions

(18) Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editingNat CommunAugust 14, 2023Sandra Wimberger, Nina Akrap, Mike Firth, Johan Brengdahl, Susanna Engberg, Marie K. Schwinn, Michael R. Slater, Anders Lundin, Pei-Pei Hsieh, Songyuan Li, et al.Scientific), passed through a 35 µm mesh filter (Corning, Falcon) to disperse clumps, and loaded onto the BD FACSMelody (BD Biosciences) cell sorter. Single cells were sorted into each well of solid white 96-well tissue culture

(19) Basonuclin-2 regulates extracellular matrix production and degradationLife Sci AllianceAugust 3, 2023Ayla Orang, B Kate Dredge, Chi Yau Liu, Julie M Bracken, Chun-Hsien Chen, Laura Sourdin, Holly J Whitfield, Rachael Lumb, Sarah T Boyle, Melissa J Davis, et al.(BD Biosciences) and sorted based on GFP or m-Cherry intensity compared with parental cells using a BD FACSMelody Cell Sorter. Where single clones were needed, pooled cells were sorted into single cells in 96-well plates and grown

(20) Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrestLife science allianceAugust 1, 2023Acharya BR, Fang JS, Jeffery ED, Chavkin NW, Genet G, Vasavada H, Nelson EA, Sheynkman GM, Humphries MJ, Hirschi KK.mVenus were used to determine the cell cycle state during flow cytometry analysis at BD FACSAria or BD FACSMelody. NivaniFlex mouse–rabbit Proximity Ligation Assay Cultured HUVEC on glass coverslips was fixed with

Be the First to Write a Review!

BD FACSMelody™ Cell Sorter 1

BD FACSMelody™ Cell Sorter 2

BD FACSMelody™ Cell Sorter 3

BD FACSMelody™ Cell Sorter 4

BD FACSMelody™ Cell Sorter 5

BD FACSMelody™ Cell Sorter from BD Biosciences

Description

BD Biosciences

Citations (193)

Reviews

BD FACSMelody™ Cell Sorter from BD Biosciences

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map