XbaI from New England Biolabs

Product Specs
ItemXbaI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0145L
Concentration20-100 u/µL
Quantity15000 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

T/CTAGA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dimerization of melanocortin 4 receptor controls puberty onset and body size polymorphismFront Endocrinol (Lausanne)November 10, 2023Ruiqi Liu, Mike Friedrich, Katherina Hemmen, Kerstin Jansen, Mateus C. Adolfi, Manfred Schartl, Katrin G. Heinzerestriction enzymes HindIII (R3104S, New England Biolabs, Ipswich, Massachusetts, United States) and XbaI (R0145S, New England Biolabs), and ligated to backbones pcDNA3-eGFP/mCherry in frame with eGFP/mCherry encoding

(2) An efficient cloning method to expand vector and restriction site compatibility of Golden Gate AssemblyCell Reports MethodsAugust 22, 2023Masato Sorida, Roberto Bonasio,Thermo Scientific,SM1331 BsaI-HFv2,NEB,R3733S BbsI-HF,NEB,R3539S AgeI-HF,NEB,R3552S BamHI-HF,NEB,R3136S EcoRI-HF,NEB,R3101L HindIII-HF,NEB,R3104S NcoI-HF,NEB,R3193S NotI-HF,NEB,R3189S SalI-HF,NEB,R3138S SpeI-HF,NEB,R3133S XbaI,NEB,R0145S XhoI,NEB,R0146S XmaI,NEB,R0180S KpnI-HF,NEB,R3142S NdeI,NEB,R0111S Smal,NEB,R0141S BbsI-HF,NEB,R3539M T4 DNA Ligase

(3) Differentiation block in acute myeloid leukemia regulated by intronic sequences of FTOiScienceAugust 18, 2023Francesco Camera, Isabel Romero-Camarero, Bradley H. Revell, Fabio M.R. Amaral, Oliver J. Sinclair, Fabrizio Simeoni, Daniel H. Wiseman, Lovorka Stojic, Tim C.P. SomervailleGreen,ThermoFisher,Cat#4309155 May-Grunwald Giemsa,Sigma,Cat#MG500 Alt-R Cas9,Integrated DNA Technologies,Cat#1081059 XbaI,NEB,Cat#R0145L SbfI,NEB,Cat#R3642L Age1,NEB,Cat#R3552S EcoR1-HF,NEB,Cat#R3101L DpnII,NEB,Cat#R0543S NlaIII,NEB,Cat#R0125S ,, Critical

(4) Fluorescent PCR-based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in ZebrafishBio ProtocAugust 5, 2023Blake Carrington, Raman Soodplasmid (Addgene, catalog number: 46757)XbaI and CutSmart buffer (New England Biolabs, catalog number: R0145S)QIAquick PCR Purification kit (Qiagen, catalog number: 28104)mMessage mMachine T3 Transcription kit

(5) Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24iScienceJuly 21, 2023Patel LR, Stratton SA, McLaughlin M, Krause P, Allton K, Rivas AL, Barbosa D, Hart T, Barton MC.CutSmart Buffer,NEB,B7204 Esp3I,Thermo,ER0452 EcoRI,NEB,R0101S BamHI,NEB,R0136S T4 Ligase,NEB,M0202S XbaI,NEB,R0145S HindIII,NEB,R0104S Q5 Site Direct Mutagenesis Kit,NEB,E0554 Herculase II Polymerase,Agilent,600675 Clontech

(6) Systematic characterization of regulatory variants of blood pressure genesCell GenomicsJuly 12, 2023Winona Oliveros, Kate Delfosse, Daniella F. Lato, Katerina Kiriakopulos, Milad Mokhtaridoost, Abdelrahman Said, Brandon J. McMurray, Jared W.L. Browning, Kaia Mattioli, Guoliang Meng, et al.,STEMCELL Technologies,Cat#05010 SfiI,NEB,Cat#R0123L "Alkaline Phosphatase, Calf Intest",NEB,Cat#M0290L T4 DNA Ligase,NEB,Cat#M0202M XbaI,NEB,Cat#R0145L KpnI-HF,NEB,Cat#R3142L Q5- High Fidelity DNA polymerase,NEB,Cat#M0491L Formamide (Deionized),Invitrogen,Cat#AM9342 Agencourt

(7) Large-scale Purification of Type III Toxin-antitoxin Ribonucleoprotein Complex and its Components from Escherichia coli for Biophysical StudiesBIO-PROTOCOLJuly 5, 2023Parthasarathy Manikandan, Kavyashree Nadig, Mahavir SinghLaboratories, catalog number: 21113, CAS number: 57-13-6)XhoI (New England Biolabs, catalog number: R0145S), store at -20 °Cβ-Mercaptoethanol (Sisco Research Laboratories, catalog number: 83759, CAS number:

(8) ERα-associated translocations underlie oncogene amplifications in breast cancerNatureJune 29, 2023Jake June-Koo Lee, Youngsook Lucy Jung, Taek-Chin Cheong, Jose Espejo Valle-Inclan, Chong Chu, Doga C. Gulhan, Viktor Ljungström, Hu Jin, Vinayak V. Viswanadham, Emma V. Watson, et al.endogenous SHANK2 and RARA locus, ligation reactions were digested with XbaI (New England Biolabs, R0145L) for SHANK2 locus and EcoRI (New England Biolabs, R0101L) for RARA locus, respectively. In the first

(9) eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome contentThe EMBO journalJune 15, 2023Duan H, Zhang S, Zarai Y, Öllinger R, Wu Y, Sun L, Hu C, He Y, Tian G, Rad R, et al.,New England Biolabs,Cat # R0580S BbSI,New England Biolabs,Cat # R0539S XbaI,New England Biolabs,Cat # R0145S XhoI,New England Biolabs,Cat # R0146S BamHI,New England Biolabs,Cat # R3136S T4 Polynucleotide Kinase,New England Biolabs

(10) Regulatory architecture of housekeeping genes is driven by promoter assembliesCell reportsMay 30, 2023Dejosez M, Dall'Agnese A, Ramamoorthy M, Platt J, Yin X, Hogan M, Brosh R, Weintraub AS, Hnisz D, Abraham BJ, et al.pTZ2073 to shorten the sequence between the Max and Fntb promoters. pTZ2073 was cut with XbaI (NEB, R0145S) and NheI (NEB, R0131L) and the 5383 bp fragment was religated after gel electrophoresis and purification

(11) TAR Syndrome-associated Rbm8a deficiency causes hematopoietic defects and attenuates Wnt/PCP signalingbioRxivMay 5, 2023Agnese Kocere, Elena Chiavacci, Kevin Manuel Méndez-Acevedo, Charlotte Soneson, Max S. Hiltabidle, Azhwar Raghunath, Jacalyn S. MacGowan, Jordan A. Shavit, Daniela Panáková, Margot L. K. Williams, et al.restriction digest into Tol2kit vector 396 (pCS2FA:Tol2) (Kwan et al., 2007) cut with BamHI and XbaI (R0145S, NEB). Morpholinos were obtained from Gene Tools (Philomath, USA) and prepared as a 1 mM stock solution

(12) Production and characterization of cell-penetrating recombinant botulinum neurotoxin type ASTAR protocolsMarch 17, 2023Wei X, Hu J, Jiang L, Li L, Liu J.Phanta Max Super-Fidelity DNA Polymerase (1 unit/μL),Vazyme,P505-d3 XbaI (20 units/μL),New England Biolabs,R0145L HindIII (20 units/μL),New England Biolabs,R0104L Cutsmart buffer 10×,New England Biolabs,B7204V Quick CIP

(13) Multimodal CRISPR perturbations of GWAS loci associated with coronary artery disease in vascular endothelial cellsPLOS GeneticsMarch 16, 2023Florian Wünnemann, Thierry Fotsing Tadjo, Mélissa Beaudoin, Simon Lalonde, Ken Sin Lo, Benjamin P. Kleinstiver, Guillaume Lettrethe gateway cassette from pLVX-EF1α-attR1-ccdB-attR2-IRES-puro-emGFP digested with XbaI (NEB, cat no R0145S) and ligated with Quick ligase from NEB (cat no M2200) in pLVX-EF1α-IRES-mCherry (refer as Empty-mCherry

(14) Prediction and verification of glycosyltransferase activity by bioinformatics analysis and protein engineeringSTAR ProtocolsMarch 1, 2023Dietlind L. Gerloff, Elena I. Ilina, Camille Cialini, Uxue Mata Salcedo, Michel Mittelbronn, Tanja MüllerV717A UDP-glucose,Sigma-Aldrich,Cat# U4625 Ethidium bromide,Bio-Rad,Cat#1610433EDU Gel loading dye 6×,NEB,Cat# B7024S XbaI,NEB,Cat #R0145S NotI-HF,NEB,Cat#R3189S ,, Critical commercial assays,Critical commercial assays,Critical commercial

(15) Protocol for investigating the biogenesis of SARS-CoV-2 S pseudoviruses in HEK293T cells transduced to express the virus-specific intrabodiesSTAR ProtocolsMarch 1, 2023Surbhi Dahiya, Sudhakar Singh, Sharvan SehrawatFemtoLUCENT plus AP kit,G-Biosciences,Cat#786-10AP PEI branched,Sigma,408727-100mL BamHI,New England Biolab (NEB),R3136S XbaI,NEB,R0145S NcoI,NEB,R3193S NotI,NEB,R3189S MG132,Sigma,474787 Ni-NTA probes,ForteBio,18-5101 Clarity Western

(16) Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike proteinSTAR ProtocolsMarch 1, 2023Angela Rose Liu, Shrikanth C. Basavarajappa, Nandini Sarkar, Anna Bruchez, Parameswaran RamakrishnanBiomedicals,802829 TEMED,Bio-Rad,161-0801 25% Glutaraldehyde,Sigma,354400 Xho1,NEB,R0146L Xba1,NEB,R0145L HindIII,NEB,R0104L Agarose,Fisher,BP160-500 Ethidium bromide,Sigma,E8751 Ligafast Rapid DNA Ligation System

(17) LINE1-Mediated Reverse Transcription and Genomic Integration of SARS-CoV-2 mRNA Detected in Virus-Infected but Not in Viral mRNA-Transfected CellsVirusesFebruary 25, 2023Liguo Zhang, Punam Bisht, Anthony Flamier, M. Inmaculada Barrasa, Max Friesen, Alexsia Richards, Stephen H. Hughes, Rudolf JaenischORF to the ending 25-nt poly-A was cloned into a pGEM-7Zf(+) vector (Promega; P2251) by XbaI (NEB; R0145L) and BamHI (NEB; R3136L) restriction cloning. This plasmid was linearized by BamHI restriction digestion,

(18) CRISPR-associated type V proteins as a tool for controlling mRNA stability in S. cerevisiae synthetic gene circuitsNucleic Acids ResearchFebruary 22, 2023Lifang Yu, Mario Andrea Marchisio-Xbal-yEGFP-CYC1t-SacI, which host the FnCas12a direct repeat on pTEF2 5′UTR, was digested with Xbal (NEB-R0145S) and SacI-HF. Then, the cut-open vector was assembled, via Gibson method, with a PCR product consisting

(19) A de novo missense variant in EZH1 associated with developmental delay exhibits functional deficits in Drosophila melanogastermedRxivFebruary 7, 2023Sharayu Jangam, Lauren C. Briere, Kristy Jay, Jonathan C Andrews, Melissa A. Walker, Lance H. Rodan, Frances A. High, Shinya Undiagnosed Diseases Network, David A. Sweetser, David A. Sweetser, et al.wild-type and variant constructs underwent restriction digestion with NotI (NEB- R3189S) and XbaI (NEB-R0145S) and then ligated into the pattB-Basler vector with the T4 DNA ligase (NEB M0202S). Sequenced verified

(20) Genome-Engineered mpkCCDc14 Cells as a New Resource for Studying AQP2International Journal of Molecular SciencesJanuary 14, 2023Hyo-Ju Jang, Hye-Jeong Park, Hong Seok Choi, Hyun Jun Jung, Tae-Hwan KwonKorea), was utilized to produce Cas9 mRNA. The Cas9 plasmid was digested into a linear form with XbaI (R0145S, New England Biolabs, Ipswich, MA, USA) and purified by phenol/chloroform (P2069, Sigma, St. Louis,

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(1)

9/11/2015Good XbaI Restriction Enzyme J GThis is a good source of the XbaI restriction enzyme. I use this product when doing diagnostic restriction digests or in molecular cloning when I need to cut out an insert.

XbaI

XbaI from New England Biolabs

Sequence

New England Biolabs

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XbaI from New England Biolabs

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