Trypsin-EDTA (0.25%), phenol red from Thermo Fisher Scientific

Product Specs
ItemTrypsin-EDTA (0.25%), phenol red
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number25200114
Quantity20 x 100mL
ApplicationsCELL CULTURE
Brand/TrademarkGibco™
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) c-Src regulates δ-secretase activation and truncated Tau production by phosphorylating the E3 ligase Traf6Journal of Biological ChemistryDecember 1, 2023Yanli Jiang, Longfei Li, Ruozhen Wu, Liulin Wu, Bin Zhang, Jian-Zhi Wang, Rong Liu, Fei Liu, Jing Wang, Xiaochuan Wangmedium was changed daily, and the cells were passaged every 5 to 6 days using 0.25% trypsin–EDTA (Gibco; 25200114) according to the manufacturer’s instructions. Neural progenitor cell induction from hiPSCs Neural

(2) Decellularised spinal cord matrix manipulates glial niche into repairing phase via serglycin-mediated signalling pathwayCell proliferationSeptember 1, 2023Zhang S, Zhai M, Xu Y, Han J, Chen J, Xiong Y, Pan S, Wang Q, Yu C, Rao Z, et al.hydrogel with cells was digested by collagenase (Sigma‐Aldrich, C9407) and trypsin (Thermo Fisher, 25200114). Digestion was neutralized with DMEM supplemented with 10% FBS. After centrifugation (300 g, 5 min),

(3) Mitochondrial Peptide Humanin Facilitates Chemoresistance in Glioblastoma CellsCancersAugust 11, 2023Peña Agudelo JA, Pidre ML, Garcia Fallit M, Pérez Küper M, Zuccato C, Nicola Candia AJ, Marchesini A, Vera MB, De Simone E, Giampaoli C, et al.Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat# A14132-02), penicillin–streptomycin (Cat# 15140122), trypsin–EDTA (0.025%, Cat# 25200114) and Lipofectamine 2000 (Cat# 11668019) were obtained from Gibco (Invitrogen, Carlsbad, CA, USA). Fetal

(4) The Effects of Thermocycling on the Physical Properties and Biocompatibilities of Various CAD/CAM Restorative MaterialsPharmaceuticsAugust 10, 2023Se-Young Kim, Han-Jin Bae, Hae-Hyoung Lee, Jong-Hyuk Lee, Yu-Jin Kim, Yu-Sung Choi, Jung-Hwan Lee, Soo-Yeon Shinwere performed at 4 and 24 h after seeding. The cells were then separated with 0.25% trypsin-EDTA (#25200114, Gibco™, Grand Island, NY, USA) and passaged when the cell confluence reached 80–90%, and passages 5–10

(5) Dissecting the spermatogonial stem cell niche using spatial transcriptomicsCell reportsJuly 25, 2023Rajachandran S, Zhang X, Cao Q, Caldeira-Brant AL, Zhang X, Song Y, Evans M, Bukulmez O, Grow EJ, Nagano M, et al.digested sequentially with 2 mg/ml Collagenase IV (LS004188, Worthington Biochemical), 0.25% Trypsin-EDTA (25200114, Thermo Fisher), and 3.5 mg/ml DNase I (LS002147, Worthington Biochemical) in HBSS at 37°C. The cell

(6) A Cell Cycle-Dependent Ferroptosis Sensitivity Switch Governed by EMP2bioRxiv : the preprint server for biologyJuly 19, 2023Rodencal J, Kim N, Li VL, He A, Lange M, He J, Tarangelo A, Schafer ZT, Olzmann JA, Sage J, et al.μM), pimasertib (0.5 μM), Dox (0.1 μg/mL). Cells were then trypsinized using 0.25% trypsin-EDTA (Cat# 25200114, Thermo Fisher Scientific) and washed 2x with 1.5 mL cold HBSS (Cat# 14025134, Life Technologies). Cells

(7) Chromatin accessibility dynamics of neurogenic niche cells reveal defects in neural stem cell adhesion and migration during agingNature AgingJuly 13, 2023Robin W. Yeo, Olivia Y. Zhou, Brian L. Zhong, Eric D. Sun, Paloma Navarro Negredo, Surag Nair, Mahfuza Sharmin, Tyson J. Ruetz, Mikaela Wilson, Anshul Kundaje, et al.with Trypsin-EDTA (referred to as trypsin in the paper; 0.25%) phenol red (Thermo Fisher Scientific, 25200114; instead of Accutase) for 15 min at room temperature to lift the cells. Trypsin, a stronger dissociation

(8) PIE-seq: identifying RNA-binding protein targets by dual RNA-deaminase editing and sequencingNature CommunicationsJune 6, 2023Xiangbin Ruan, Kaining Hu, Xiaochang ZhangMagnesium (Gibco 14190250) 24 or 48 h after transfection and digested with 0.25% Trypsin-EDTA (Gibco 25200114). Cells were diluted with 1x DPBS supplemented with 10% FBS and filtered through 70-µm cell strainers

(9) Growth State-Dependent Activation of eNOS in Response to DHA: Involvement of p38 MAPKInternational journal of molecular sciencesMay 6, 2023Huang S, Taylor CG, Zahradka P.Cell Cycle Analysis Treated EA.hy926 cells on 6-well plates were harvested with trypsin (Catalog #: 25200114, Gibco™, Waltham, MA, USA) for cell cycle analysis using flow cytometry as previously described [46].

(10) Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirusNature CommunicationsMay 5, 2023Pengcheng Shang, Joshua D. Simpson, Gwen M. Taylor, Danica M. Sutherland, Olivia L. Welsh, Pavithra Aravamudhan, Rita Dos Santos Natividade, Kristina Schwab, Joshua J. Michel, Amanda C. Poholek, et al.the remaining tissue was sectioned into fine pieces and dissociated with 0.25% trypsin-EDTA (GIBCO, #25200114). Dissociated primary embryonic fibroblasts were propagated in tissue-culture flasks. MEFs from passage

(11) Neuron stem cell NLRP6 sustains hippocampal neurogenesis to resist stress-induced depressionActa Pharmaceutica Sinica BMay 1, 2023Chuanfeng Tang, Qiaona Wang, Jingyan Shen, Congying Wang, Hong Ding, Shiyu Wen, Fan Yang, Ruiqing Jiao, Xingxin Wu, Jianmei Li, et al.removed, and hippocampus sample was transferred to 15 mL-centrifuge tube and digested with 0.25% trypsin (25200114, Gibco) in 5% CO2 incubator for 20 min. Then the trypsin was aspirated, and sample was washed by DMEM/F-12

(12) Integrative multi-omics and drug-response characterization of patient-derived prostate cancer primary cellsSignal transduction and targeted therapyMay 1, 2023Wang Z, Li Y, Zhao W, Jiang S, Huang Y, Hou J, Zhang X, Zhai Z, Yang C, Wang J, et al.80% to 90% confluency, epithelial cells were enzymatically detached with 0.25% trypsin-EDTA (Gibco, 25200114) and passaged at a ratio of 1:3. Fresh primary medium was changed every 2 to 3 days. Most primary cells

(13) The ER membrane protein complex governs lysosomal turnover of a mitochondrial tail-anchored protein, BNIP3, to restrict mitophagybioRxiv : the preprint server for biologyMarch 24, 2023Delgado JM, Wallace Shepard L, Lamson SW, Liu SL, Shoemaker CJ.confluency and passaged less than 25 times. For passaging, cells are trypsinized with 0.25% Trypsin-EDTA (25200114, Thermo Scientific). For hypoxia incubation, cells were incubated in a humidified Baker Ruskinn InvivO2

(14) Rapid and effective preparation of clonal bone marrow-derived mesenchymal stem/stromal cell sheets to reduce renal fibrosisScientific reportsMarch 17, 2023Kameishi S, Dunn CM, Oka M, Kim K, Cho YK, Song SU, Grainger DW, Okano T.°C, 5% CO2. Expanded cBMSCs were then collected using 0.05% trypsin EDTA (Thermo Fisher Scientific, 25200114), centrifuged at 210xg for 5 min, and resuspended in STEM-CELLBANKER cryogenic media (Amsbio, 11890)

(15) MerlinS13 phosphorylation controls meningioma Wnt signaling and magnetic resonance imaging featuresResearch SquareMarch 15, 2023Charlotte Eaton, Lauro Avalos, S. John Liu, Tim Casey-clyde, Paola Bisignano, Calixto-Hope Lucas, Erica Stevenson, Abrar Choudhury, Harish Vasudevan, Stephen Magill, et al.Biochemicals, Cat# LS005332) at 37°C for 30 minutes, followed by 0.25% trypsin (Thermo Fisher Scientific, Cat# 25200114) digest at 37°C for 10 minutes. Red blood cells were removed in 1X RBC lysis buffer (Invitrogen, Cat#

(16) Tandem mass tag-based thermal proteome profiling for the discovery of drug-protein interactions in cancer cellsSTAR ProtocolsMarch 1, 2023Fraser D. Johnson, Christopher S. Hughes, Alvin Liu, William W. Lockwood, Gregg B. Morinproteins" ,, Dulbecco’s phosphate buffered saline,Gibco,Cat# 14190250 0.25% Trypsin-EDTA,Gibco,Cat# 25200114 RPMI media,Gibco,Cat# 11875119 Fetal bovine serum,Gibco,Cat# 1283020 Bovine serum albumin,Sigma,Cat#

(17) Cytokinesis machinery promotes cell dissociation from collectively migrating strands in confinementScience AdvancesJanuary 13, 2023Robert A. Law, Alexander Kiepas, Habben E. Desta, Emiliano Perez Ipiña, Maria Parlani, Se Jong Lee, Christopher L. Yankaskas, Runchen Zhao, Panagiotis Mistriotis, Nianchao Wang, et al.device seeding and live-cell imaging Cells were harvested from culture flasks with 0.25% trypsin-EDTA (25200114, Gibco), centrifuged at 300g for 5 min, and resuspended at a concentration of 5 × 107 cells/ml in serum-containing

(18) Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneityCell reportsDecember 6, 2022Kaserman JE, Werder RB, Wang F, Matte T, Higgins MI, Dodge M, Lindstrom-Vautrin J, Bawa P, Hinds A, Bullitt E, et al.Y-27632 dihydrochloride,Tocris,1254 Primocin,InvivoGen,ant-pm "Trypsin-EDTA (0.25%), phenol red",ThermoFisher Scientific,25200114 ClaI restriction enzyme,New England BioLabs,R0197 N-2 Supplement,ThermoFisher Scientific,17502048 "B27

(19) Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9Scientific ReportsDecember 1, 2022Matthew T. Biegler, Olivier Fedrigo, Paul Collier, Jacquelyn Mountcastle, Bettina Haase, Hagen U. Tilgner, Erich D. Jarvisining embryonic skin and muscle tissue was pipetted vigorously in 0.25% Trypsin–EDTA (Gibco, Cat. #25200114) and left to incubate at 37 °C for five minutes. The tubes were centrifuged at 200×g for 3 min and resuspended

(20) Using the Sleeping Beauty (SB) Transposon to Generate Stable Cells Producing Enveloped Virus-Like Particles (eVLPs) Pseudotyped with SARS-CoV-2 Proteins for VaccinationCurr ProtocOctober 1, 2022Pszenny V, Tjhin E, Alves-Ferreira EVC, Spada S, Bouamr F, Nair V, Ganesan S, Grigg ME.12B). Additional Materials (also see Basic Protocol 5) Trypsin‐EDTA (0.25%), phenol red (Gibco, cat. no. 25200114)Five‐layer tissue culture flasks (Falcon® 875 cm² Cell Culture Multi‐Flask, 5‐layer with Vented Cap,

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Trypsin-EDTA (0.25%), phenol red from Thermo Fisher Scientific

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Trypsin-EDTA (0.25%), phenol red from Thermo Fisher Scientific

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