- TRIsure
- Bioline
- Pricing InfoSupplier PageView Company Product Page
- BIO-38032
- 100ml
- From 1mg of tissue or 1 x 106 of cultured cells, the expected yield of RNA is:
- 2-5µg from mouse kidney (tissue)
- 5-10µg from mouse liver (tissue)
- 8-15µg from epithelial cells (cultured cells)
- 20-25µg from fibroblasts (cultured cells)
- 1hr protocol
Product Description
TRIsure is a ready-to-use reagent for the isolation of total RNA from cells and tissues. TRIsure maintains the integrity of the extracted RNA, while disrupting cells and subsequently dissolving cell components. After the addition of chloroform, the RNA can be recovered easily from the aqueous phase. DNA and protein can also be recovered after further precipitation steps. The RNA remains in the aqueous phase and is subsequently recovered by precipitation with isopropyl alcohol. The isolated RNA is suitable for any downstream application such as RT-PCR, hybridization assays, or in vitro translation. 1ml of TRIsure is sufficient to isolate total RNA from 1 x 107 cells or 100mg of tissue
Specifications/Features
Phenol / Chloroform:
Yes
Starting Amount:
1 x 107 cells or 100mg of tissue