S1000™ Thermal Cyclers from Bio-Rad

Product Specs
ItemS1000™ Thermal Cyclers
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog Number1852138 / 1852148 / 1852196 / 1852197
Wells96, 384 and Dual 48/48 wells
Temperature Range0 to 100 °C
Temperature Uniformity±0.4°C well-to-well within 10 sec of arrival at 90°C
Lid TypeFully adjustable heated lid
Dimensions(WxDxH) 33 x 46 x 20 cm (13 x 18 x 8 inches)
ApplicationsAmplification/PCR, Cloning, Cycle sequencing, Gene expression studies, Mutagenesis
QuantityEA
Heating Rate2.5°C/sec (Max)
Cooling Rate2.5°C/sec (Max)

Features

Temperature gradient

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Cytokinin Promotes Jasmonic Acid Accumulation in the Control of Maize Leaf GrowthPlants (Basel)August 21, 2023Aimee N. Uyehara, Angel R. Del Valle-Echevarria, Charles T. Hunter, Hilde Nelissen, Kirin Demuynck, James F. Cahill, Zachary Gorman, Georg Jander, Michael G. MuszynskiMiddleton, WI, USA) and following the manufacturer’s recommendations. The RT-PCR was performed using S1000™ Thermal Cyclers (Bio-Rad; Hercules, CA, USA) using the following cycling program: step 1 = 98 °C for 2 min, step 2 =

(2) GTS-21 Enhances Regulatory T Cell Development from T Cell Receptor-Activated Human CD4+ T Cells Exhibiting Varied Levels of CHRNA7 and CHRFAM7A ExpressionInternational Journal of Molecular SciencesJuly 31, 2023Masato Mashimo, Takeshi Fujii, Shiro Ono, Yasuhiro Moriwaki, Hidemi Misawa, Tetsushi Azami, Tadashi Kasahara, Koichiro Kawashimaprepared by reverse transcription using a Prime Script RT reagent Kit (Takara Bio., Shiga, Japan) in a S1000 Thermal Cycler (Bio-rad, Hercules, CA, USA). Real-time PCR analysis was conducted using TB Green Premix Ex Taq II,

(3) Black Ginseng Extract Exerts Potentially Anti-Asthmatic Activity by Inhibiting the Protein Kinase Cθ-Mediated IL-4/STAT6 Signaling PathwayInternational Journal of Molecular SciencesJuly 26, 2023Yu Na Song, Jae-Won Lee, Hyung Won Ryu, Jae Kyoung Lee, Eun Sol Oh, Doo-Young Kim, Hyunju Ro, Dahye Yoon, Ji-Yoon Park, Sung-Tae Hong, et al.Quantitative real-time polymerase chain reaction (qRT-PCR) was performed using iQ SYBR Green supermix (#1708880) and a S1000 Thermal Cycler Real-Time PCR System (Bio-Rad, Hercules, CA, USA) in the presence of cDNA (1:25 dilution) and primers

(4) Catalytic Site Proximity Profiling for Functional Unification of Sequence-Diverse Radical S-Adenosylmethionine EnzymesACS Bio & Med Chem AuJune 21, 2023Timothy W. Precord, Sangeetha Ramesh, Shravan R. Dommaraju, Lonnie A. Harris, Bryce L. Kille, Douglas A. Mitchell(University of Illinois at Urbana-Champaign). Polymerase chain reactions were performed using a Bio-Rad S1000 thermal cycler. Escherichia coli DH5α and BL21(DE3) strains were used for plasmid maintenance and protein overexpression,

(5) Defining an optimal control for RNAi experiments with adult Schistosoma mansoniScientific ReportsJune 16, 2023Max F. Moescheid, Oliver Puckelwaldt, Mandy Beutler, Simone Haeberlein, Christoph G. Greveldingat 95 °C for 30 s, primer annealing at 58 °C for 30 s and elongation at 72 °C for 0.45 min (BioRad, S1000 Thermal Cycler). Aliquots of generated PCR products were analyzed by agarose gel electrophoresis, cleaned using Monarch

(6) Development of a targeted amplicon sequencing method for genotyping Cyclospora cayetanensis from fresh produce and clinical samples with enhanced genomic resolution and sensitivityFrontiers in MicrobiologyJune 16, 2023Susan R. Leonard, Mark K. Mammel, Baback Gharizadeh, Sonia Almeria, Zhihai Ma, David J. Lipman, Mary E. Torrence, Chunlin Wang, Steven M. Musseras template in this indexing PCR step and the second half was stored at −20°C as a backup. A Bio-Rad S1000 Thermal Cycler (Bio-Rad, Hercules, CA) was used for both PCR steps. Following a SPRIselect bead purification step,

(7) The evolutionary history of Brachyury genes in Hydrozoa involves duplications, divergence, and neofunctionalizationScientific ReportsJune 9, 2023Alexandra A. Vetrova, Daria M. Kupaeva, Alena Kizenko, Tatiana S. Lebedeva, Peter Walentek, Nikoloz Tsikolia, Stanislav V. KremnyovPCR plates (Brand #781366). Conventional PCR and gel-electrophoresis was conducted analogously on a S1000 Thermal cycler (Bio-Rad). See Table 1 for gene-specific primers. Expression values were normalized against two housekeeping

(8) Cul3 substrate adaptor SPOP targets Nup153 for degradationbioRxivJune 4, 2023Joseph Y. Ong, Jorge Z. Torresand Software,, S1000 Thermal Cycler with Dual 48/48 Fast Reaction Module,BIORAD Life Science Group,1852148 "CFX Connect Real-Time PCR Detection System; software: CFX Manager, Version 3.0",BIORAD Life Science

(9) Autophagy gene Atg7 regulates the development of radiation-induced skin injury and fibrosis of skinSkin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)June 1, 2023Chen X, Qin W, Wang L, Jin Y, Tu J, Yuan X.s, and 72°C for 1 min, with final extension at 72°C for 5 min. DNA amplification was conducted in a S1000 Thermal Cycler (Bio‐Rad, USA). Amplified fragments were separated on a 2% agarose gel containing SuperRed/GelRed (Biosharp,

(10) Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustrisSTAR ProtocolsJune 1, 2023Mark Kathol, Cheryl Immethun, Rajib SahaTube,Corning®,CAT#3213 NanoDrop One,Thermo Scientific™,CAT#ND-ONE-W SYBR™ Safe DNA Gel Stain,Invitrogen™,CAT#S33102 S1000™ Thermal Cycler,Bio-Rad,https://www.bio-rad.com/en-us/product/s1000-thermal-cycler?ID=ab2cabb3-9299-489f-93fe-754e14301ac6 PowerUp™ SYBR™ Green Master Mix

(11) The Expression of the Endocannabinoid Receptors CB2 and GPR55 is Highly Increased during the Progression of Alzheimer’s Disease in AppNL-G-F Knock-In MiceBiology (Basel)May 31, 2023Dina Medina-Vera, Hongjing Zhao, Erika Bereczki, Cristina Rosell-Valle, Makoto Shimozawa, Gefei Chen, Fernando Rodríguez de de Fonseca, Per Nilsson, Simone Tambaroreverse transcripted to cDNA. The RNA was mixed with 20μL of master mix and amplified by using a cycler (S1000 Thermal cycler Bio-Rad, Hercules, CA, USA). For the RT-qPCR a total of 10 μL reactions were run in duplicates using

(12) Genetic Diversity and Population Structure of Leishmania infantum in Morocco as Revealed by Multilocus Sequence Typing (MLST) ApproachPathogensMay 31, 2023Sara El Mazini, Mourad Barhoumi, Idris Mhaidi, Othmane Daoui, Mouad Ait Kbaich, Sofia El Kacem, Imane El idrissi Saik, Myriam Riyad, Khadija Bekhti, Ikram Guizani, et al.μL template DNA and distilled water to reach 25 μL. The amplification reactions were conducted in an S1000 thermal cycler (Bio Rad, Hercules, CA, USA) following a cycling program for denaturation at 95 °C for 4 min, then 30

(13) Identification and in vitro and in vivo validation of the key role of GSDME in pyroptosis-related genes signature in hepatocellular carcinomaBMC CancerMay 6, 2023Xinyi Chen, Mu Yang, Lu Wang, Yuan Wang, Jingyao Tu, Xiao Zhou, Xianglin YuancDNA synthesis utilizing a Vazyme HiScript Q RT SuperMix for qPCR (Vazyme, Nanjing, China) through S1000 Thermal Cycler (Bio-Rad, USA), as instructed by the manufacturer. qPCR was done on a 7900HT Fast Real-Time PCR System

(14) Regorafenib induces NOX5-mediated endoplasmic reticulum stress and potentiates the anti-tumor activity of cisplatin in non-small cell lung cancer cellsNeoplasia (New York, N.Y.)May 1, 2023Sui H, Xiao S, Jiang S, Wu S, Lin H, Cheng L, Ye L, Zhao Q, Yu Y, Tao L, et al.Carlsbad, CA, USA). cDNA was synthesized from total RNA using the PrimeScript™ RT Master Mix (TaKaRa) on S1000 Thermal Cycler (Bio-Rad, USA). According to the manufacturer's instructions, each PCR reaction was performed using

(15) An in vitro model for vitamin A transport across the human blood-brain barrierbioRxivApril 18, 2023Chandler B. Est, Regina M. Murphyratio of > 1.8. 200 ng of purified RNA was immediately reverse transcribed at 37 °C for one hour in an S1000 Thermal Cycler (BioRad) via the OmniScript RT Kit (Qiagen) according to the manufacturer’s instructions using RNase

(16) Attraction, mobility, and preference by Lasioderma serricorne (Coleoptera: Ptinidae) to microbially-mediated volatile emissions by two species of fungi in stored grainScientific ReportsApril 15, 2023Marco A. Ponce, Petra Sierra, Jacqueline M. Maille, Tania N. Kim, Erin D. Scully, William R. Morrisonof 95 °C for 30 s, 55 °C for 1 min, and 72 °C for 1.5 min, and a final extension at 72 °C for 5 min (S1000 Thermal Cycler, Bio-Rad, Hercules, CA, USA). PCR products were treated with ExoSAP-it prior to sequencing following

(17) Novel Multiplex PCR Assay and Its Application in Detecting Prevalence and Antibiotic Susceptibility of Porcine Respiratory Bacterial Pathogens in Guangxi, ChinaMicrobiology SpectrumApril 13, 2023Jing Rao, Xinchen Wei, Huan Li, Zhewei Zhang, Jiahui Liu, Mengjie Lian, Weiwei Cao, Long Yuan, Beibei Dou, Yanhong Tian, et al.suis, P. multocida, and M. hyopneumoniae are provided in Table 4. PCR was performed with a Bio-Rad S1000 thermal cycler (Bio-Rad, USA) in a 20-μL reaction system containing 10 μL of 2× Taq PCR Master Mix (Vazyme, China),

(18) Amniogenesis in Human Amniotic Sac Embryoids after Exposures to Organophosphate Flame RetardantsEnvironmental Health PerspectivesApril 7, 2023Chenke Xu, Chenhao Zhang, Yanan Liu, Haojia Ma, Feifan Wu, Yingting Jia, Jianying HuTakara), dNTP (4019; Takara), and Random Primers (3802; Takara) were used for reverse transcription on a S1000 Thermal Cycler (Bio-Rad) with the program: 40°C for 60 min, 70°C for 15 min, and 4°C for 30 min. We used SYBR Green

(19) Cholinergic regulation of vascular endothelial function by human ChAT + T cellsProceedings of the National Academy of SciencesApril 4, 2023Laura Tarnawski, Vladimir S. Shavva, Eric J. Kort, Zhengbing Zhuge, Ingrid Nilsson, Alessandro L. Gallina, David Martínez-Enguita, Benjamin Heller Sahlgren, Matthew Weiland, April S. Caravaca, et al.Appendix, Table S5. cDNA was preamplified using the Veriti™ 96-Well Thermal Cycler (Applied Biosystems) or S1000™ Thermal Cycler (Bio-Rad). Single-cell qPCR was performed blinded according to the manufacturer’s protocol using the

(20) QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylaseHorticulture ResearchMarch 3, 2023Pilar Muñoz, Cristina Castillejo, José Antonio Gómez, Luis Miranda, Silke Lesemann, Klaus Olbricht, Aurélie Petit, Philippe Chartier, Annika Haugeneder, Johanna Trinkl, et al.High-Capacity cDNA Reverse Transcription Kit (Applied biosystems by Thermo Fisher Scientific) in a S1000™ Thermal Cycler (Bio-Rad) following the manufacturer’s instructions. Subgenome-specific primers were designed for transcript

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(1)

3/23/202096-Well PCR Machine for Cloning and Genotyping Zan ChenThis thermal cycler is durable, temperature ramp is fast, and easy to operate. The price is also reasonable.
(1)

6/19/2018Thermal Cycler For PCR Irene LewThermal cycler is used for polymerase chain reaction and cDNA synthesis. The machine is dependable and makes reproducible strong bands. This model comes with a variety of functions but the best one is... Read Review
(1)

6/30/2017Excellent Bio-Rad Thermal Cycler Frank WuOur lab is heavily reliant on thermal cyclers for PCR mice genotyping and cDNA synthesis. This instrument is probably in use nearly all the time during the workday and it's making holding up ... Read Review
(1)

4/27/2017Reliable And Versatile Thermocycler Liz G.Our lab does a good amount of molecular biology such as amplification of 16S sequences of archaeal organisms and mixed microbial populations. This instrument has reliably carried out PCR-related ... Read Review
(1)

7/11/2016Excellent PCR Machine Tima ThomasThis PCR machine is useful for PCR, cloning and sequencing studies.