T4 DNA Ligase from New England Biolabs

Product Specs
ItemT4 DNA Ligase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0202T
Quantity20000 units
Concentration2000u/µl
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One NEB unit is defined as the amount of enzyme required to give 50% ligation of HindIIIfragments of Lambda DNA in 30 minutes at 16°C in 20 µl of the above assay mixture and a 5´ DNA terminiconcentration of 0.12 µM (300 µg/ml).

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A comprehensive in vivo screen of yeast farnesyltransferase activity reveals broad reactivity across a majority of CXXX sequencesG3: Genes, Genomes, GeneticsJuly 5, 2023June H Kim, Emily R Hildebrandt, Anushka Sarkar, Wayland Yeung, La Ryel A Waldon, Natarajan Kannan, Walter K Schmidtre-purified, and ligated into pWS1728 (BsrGI and AflII digested) using high-concentration T4 DNA ligase (NEB M0202T). Ligations were transformed into DH5α Escherichia coli prepared using Z-Competent cell kit (G-Biosciences,

(2) Development of virus-like particles with inbuilt immunostimulatory properties as vaccine candidatesFrontiers in MicrobiologyJune 7, 2023Simon Collett, Linda Earnest, Julio Carrera Montoya, Melissa A. Edeling, Ashley Yap, Chinn Yi Wong, Dale Christiansen, Jason Roberts, Jamie Mumford, Valerie Lecouturier, et al.and NotI-HF (R3189S, NEB, United States) and ligated into the vector pAdTrackCMV using T4 DNA Ligase (M0202T, NEB, United States). The reaction was incubated at 16°C for 2 h and then transformed into chemically

(3) Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periodsCell Reports MethodsJune 1, 2023Fabian Otte, Yuepeng Zhang, Julian Spagnuolo, Alexander Thielen, Martin Däumer, Carsten Wiethe, Marcel Stoeckle, Katharina Kusejko, Florian Klein, Karin J. Metzner, et al.1-26F50-I PBS,Bioconcept,Cat#: 3-05F29-I "RPMI-1640,",Bioconcept,Cat#: BC-1-41F51-I T4 DNA ligase,NEB,Cat#: M0202T Taq DNA Polymerase with ThermoPol®Buffer,NEB,Cat#: M0267S FBS,Gibco,Cat#: 26140079 UltraComp eBeads™

(4) Multimodal perturbation analyses of cyclin-dependent kinases reveal a network of synthetic lethalities associated with cell-cycle regulation and transcriptional regulationScientific ReportsMay 11, 2023Kyle Ford, Brenton P. Munson, Samson H. Fong, Rebecca Panwala, Wai Keung Chu, Joseph Rainaldi, Nongluk Plongthongkum, Vinayagam Arunachalam, Jarek Kostrowicki, Dario Meluzzi, et al.reactions were then set up using 100 ng of vector, 100 ng of insert, 2 μL of buffer, 1 μL of T4 ligase (NEB M0202T), and ultra pure H2O up to 20 μL. Each reaction was allowed to proceed overnight at 16 °C. The following

(5) A trailing ribosome speeds up RNA polymerase at the expense of transcript fidelity via force and allosteryCellMarch 16, 2023Liang Meng Wee, Alexander B. Tong, Alfredo Jose Florez Florez Ariza, Cristhian Cañari-Chumpitaz, Patricia Grob, Eva Nogales, Carlos J. BustamanteStain,Thermo Fisher Scientific,Cat#S-11494 "T4 DNA Ligase (2,000,000 units/ml)",New England Biolabs,Cat#M0202T "T4 DNA Ligase (400,000 units/ml)",New England Biolabs,Cat#M0202S T4 Polynucleotide Kinase,New England Biolabs

(6) Condensin pinches a short negatively supercoiled DNA loop during each round of ATP usageThe EMBO JournalFebruary 1, 2023Belén Martínez‐García, Sílvia Dyson, Joana Segura, Alba Ayats, Erin E Cutts, Pilar Gutierrez‐Escribano, Luís Aragón, Joaquim RocaPhosphatase (NEB #M0290); Apyrase (NEB #M0398S); Endonuclease BspQI (NEB #R06445); T4 DNA ligase (NEB #M0202T). To produce a stock of relaxed DNA, 10 μg of negatively supercoiled pBR322 (4.3 Kbp) were pre‐incubated

(7) Screening and characterization of inhibitory vNAR targeting nanodisc-assembled influenza M2 proteinsiScienceJanuary 20, 2023Chuandi Yu, Wen Ding, Lei Zhu, Yuhang Zhou, Yingkui Dong, Ling Li, Juanjuan Liu, Yizhuo Wang, Zehua Li, Lina Zhu, et al.2203ML010 Freund’s Adjuvant incomplete,BIOFROXX,Cat# 1643ML010 sfiI,NEB,Cat# R0123L T4 DNA ligase,NEB,Cat# M0202T pComb3XSS,"Andris-Widhopf et al.,48",Cat# 63890 (Addgene) pcold II,Sangon,Cat# B540188-0001 "3,3′-Dioctadecyloxacarbocyanine

(8) Low input capture Hi-C (liCHi-C) identifies promoter-enhancer interactions at high-resolutionNature CommunicationsJanuary 17, 2023Laureano Tomás-Daza, Llorenç Rovirosa, Paula López-Martí, Andrea Nieto-Aliseda, François Serra, Ainoa Planas-Riverola, Oscar Molina, Rebecca McDonald, Cedric Ghevaert, Esther Cuatrecasas, et al.the adenine-tailed DNA fragments by adding 1 µl of T4 DNA ligase 2000U/µl (New England Biolabs cat. #M0202T) and 4 µl of preannealed adapter mix 15 µM and incubating the mixture 2 h at room temperature. The bead-bound

(9) A versatile active learning workflow for optimization of genetic and metabolic networksNature CommunicationsDecember 1, 2022Amir Pandi et al.(pBEAST-J23101-B0032-sfGfp)58. Using golden gate cloning (BsaI-HFⓇv2 NEB #R3733L, T4 DNA ligase NEB #M0202T), in this plasmid, the super folder Gfp gene was replaced by LacI for constitutive-LacI, then the promoter

(10) Ascertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus librariesNat CommunNovember 16, 2022Arpiar Saunders, Kee Wui Huang, Cassandra Vondrak, Christina Hughes, Karina Smolyar, Harsha Sen, Adrienne C. Philson, James Nemesh, Alec Wysoker, Seva Kashin, et al.min (heat inactivation). Step 2 = Ligation (spike-in, +5.1 μl): (1) T4 DNA Ligase (10,000 U/ml, NEB M0202T), 0.1 μl (200 U); ATP (10 mM, NEB P0756L), 5 μl. Step 2 Cycle Conditions: (1) 4 °C for 2 h; 2. 65 °C

(11) Specification of female germline by microRNA orchestrated auxin signaling in ArabidopsisNat CommunNovember 15, 2022Jian Huang, Lei Zhao, Shikha Malik, Benjamin R. Gentile, Va Xiong, Tzahi Arazi, Heather A. Owen, JiÅ™Ã Friml, Dazhong Zhaowith the annealed insert, the pHEE401E vector, BsaI-HF®v2 (NEB #R3733), and the T4 DNA Ligase (NEB # M0202T) to produce the crispr-arf17 construct. The pGWB1 binary vector was used for genetic and expression

(12) Functional analysis of the short splicing variant encoded by CHI3L1/YKL-40 in glioblastomaFront OncolNovember 2, 2022Mengqi Shi, Qianyun Ge, Xinrong Wang, Wenbin Diao, Ben Yang, Sipeng Sun, Guohui Wang, Tian Liu, Andrew Man-Lok Chan, Zhiqin Gao, et al.#R0101T) and cloned into the pBABE-puro and pBABE-Neo (Addgene, #1767) vectors using T4 DNA ligase (NEB, #M0202T). All constructs were confirmed by Sanger sequencing. Confirmed plasmids were transformed into Escherichia coli

(13) Temporal analysis suggests a reciprocal relationship between 3D chromatin structure and transcriptionCell ReportsNovember 1, 2022Kathleen S.M. Reed, Eric S. Davis, Marielle L. Bond, Alan Cabrera, Eliza Thulson, Ivana Yoseli Yoseli Quiroga, Shannon Cassel, Kamisha T. Woolery, Isaac Hilton, Hyejung Won, et al.ligase buffer,NEB,Cat# B0202S BSA (20 mg/mL),NEB,Cat# B9000S "T4 DNA ligase (2,000,000 U/mL)",NEB,Cat# M0202T Proteinase K,NEB,Cat# P8107S Agencourt AMPure XP beads,Beckman Coulter,Cat# A63881 Dynabeads MyOne Streptavidin T1

(14) STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cellsCell Rep MethodsOctober 24, 2022Blanch-Asensio A, Grandela C, Brandão KO, de Korte T, Mei H, Ariyurek Y, Yiangou L, Mol MPH, van Meer BJ, Kloet SL, et al.,New England Biolabs,Cat#R0525S BpiI,ThermoFisher,Cat#ER1011 FastDigest Eco31I,ThermoFisher,Cat#FD0293 T4 DNA Ligase,New England Biolabs,Cat#M0202T Primocin®,InvivoGen,Cat#ant-pm-05 Blasticidin S hydrochloride,Sigma–Aldrich,Cat#15205 Zeocin™ Selection Reagent

(15) Alternative cleavage and polyadenylation generates downstream uncapped RNA isoforms with translation potentialMolecular CellOctober 20, 2022Yuval Malka, Ferhat Alkan, Shinyeong Ju, Pierre-Rene KÃ¶rner, Abhijeet Pataskar, Eldad Shulman, Fabricio Loayza-Puch, Julien Champagne, Casper Wenzel, William James Faller, et al.#M0338S Harringtonine,Santa Cruz,CAT # sc-204771A CleanNGS,Gcbiotech,CAT # CNGS-0050 T4 DNA ligase,NEB,CAT #M0202T T4 RNA Ligase 1,NEB,CAT #M0204S Phusion DNA Polymerase,Thermo,CAT #F530L NEBNext End Repair,NEB,CAT

(16) A patatin-like phospholipase mediates Rickettsia parkeri escape from host membranesNature communicationsJune 27, 2022Borgo GM, Burke TP, Tran CJ, Lo NTN, Engström P, Welch MD.was heat-inactivated and DNA fragments were self-ligated using T4 DNA ligase (New England Biolabs, M0202T). E. coli were transformed with the ligation reaction and plated onto Luria-Bertani (LB) agar plates

(17) Furin extracellularly cleaves secreted PTENα/β to generate C-terminal fragment with a tumor-suppressive roleCell death & diseaseJune 6, 2022Zhang C, Ma HM, Dong SS, Zhang N, He P, Ge MK, Xia L, Yu JX, Xia Q, Chen GQ, et al.RESOURCE,SOURCE,IDENTIFIER HSA,Sino Biological,Cat# 10968-HNAY T4 DNA Ligase,New England Biolabs,M0202T TRIzol™ Reagent,Thermo,Cat# 15596026 MMLV reverse transcription reagent,Promega,Cat# M1705 NEBuilder HiFi DNA Assembly Master Mix

(18) The Arabidopsis F-box protein FBW2 targets AGO1 for degradation to prevent spurious loading of illegitimate small RNACell ReportsApril 12, 2022Thibaut Hacquard et al.fragment with overhang ends. We then ligated duplex adapter at the 3′ end using T4 DNA ligase (NEB, #M0202T) and PAGE purify the resulting fragment. Finally, we did a PCR amplification using indexed primers from

(19) Subtractive Hybridization and Construction of cDNA LibrariesMolecular EmbryologyApril 1, 2022Bruce Blumberg, Juan Carlos Izpisúa Izpisúa Belmonte

(20) A comprehensive approach for genome-wide efficiency profiling of DNA modifying enzymesCell Reports MethodsMarch 28, 2022Charalampos Kyriakopoulos et al.0.5 μL of 100 μM HP, 0.5 μL of 100 μM SA, 5 μL of 10 mM ATP and 2 μL of 2000 U/μL T4 DNA Ligase (NEB M0202T) were added for a total volume of 48 μL, ligation reaction was incubated at 16°C over night. Depletion

Write a Review

(1)

4/18/2016DNA Ligation Kit Benjamin GerovacThis is a good kit for DNA ligation. Good for ligating sticky and blunt ends with overnight incubation. Ligated DNA successfully transforms into bacteria.

Production of a Subtracted cDNA Library
July 01, 2001
Ligation of Linkers or Adapters to Double- Stranded cDNA
January 01, 1991

T4 DNA Ligase

T4 DNA Ligase from New England Biolabs

Description

Specifications/Features

New England Biolabs

Citations (99)

Reviews

Protocols

Production of a Subtracted cDNA Library

Ligation of Linkers or Adapters to Double- Stranded cDNA

T4 DNA Ligase from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map