pCR8/GW/TOPO TA Cloning Kit with One Shot Mach1-T1R E. coli and PureLink Kit from Life Technologies

pCR8/GW/TOPO TA Cloning Kit with One Shot Mach1-T1R E. coli and PureLink Kit from Life Technologies

Description

Invitrogen's pCR®8⁄GW⁄TOPO® TA Cloning Kit delivers simple 5 minute cloning, access to the Gateway® recombination cloning vector platform. Our pCR®8 TOPO® TA vector contains a minimal MCS so you sequence more of your insert and less of the vector. And with our patented att sites, the pCR®8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.

The pCR®8⁄GW⁄TOPO® TA Cloning Kit offers:

- Fast growing E. coli shortens cloning time
- Maximum convenience: includes plasmid miniprep kit
- att sites for rapid recombination into any Gateway® destination vector
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- Sequencing primer sites within 55bp of insertion site ensures you sequence more insert, less vector
- Spectinomycin resistance gene for robust selection in E. coli
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts

Specifications

  • Detailed Specifications
  • ItempCR8/GW/TOPO TA Cloning Kit with One Shot Mach1-T1R E. coli and PureLink Kit
  • CompanyLife Technologies
  • Price Pricing InfoGet Quote
  • Catalog NumberK252002
  • Quantity20 preps
  • ApplicationsCloning:Entry Vectors & Kits:Gateway Cloning:PCR Cloning:pENTR Vectors & Kits

Specifications/Features

Kit Contents:
TOPO®-adapted, ready-to-use pCR®8/GW/TOPO® Vector, dNTPs, Salt Solution, Sterile Water, GW Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot® TOP10 Chemically Competent E. coli or One Shot® Mach1™-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink™ Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes.

Life Technologies
Life Technologies
5791 Van Allen Way
Carlsbad, CA 92008
United States
Phone: (760) 603-7200

Citations