Description
BD PureCoat dish, carboxyl, 100 mm
The promise of stem cells lies in their distinctive ability to differentiate
along a multitude of cell lineages, thus considerable efforts are focused
on identifying biomaterials that promote stem cell expansion and/or
differentiation. Here, we report that BD PureCoat™ amine, a chemically
defined, animal-free, cell culture surface, supports increased growth of
human bone-marrow-derived mesenchymal stem cells (hMSC) and human
adipose-derived stem cells (hASC) culture over standard tissue culturetreated
(TC-treated) vessels. When cultured on BD PureCoat amine, these
cell types grow and reach cell confluence faster than they do on a standard
TC-treated surface while retaining established surface marker profiles that
are characteristic of these cells. Both hASC and hMSC expanded on BD
PureCoat amine for multiple passages can be induced to differentiate into
osteogenic or adipogenic lineages on TC-treated, BD PureCoat amine, and
BD PureCoat carboxyl surfaces. This application note will focus on growth
and differentiation of hMSC and hASC on BD PureCoat surfaces.
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Cultured neural cells are used in vitro as a method to study
brain function and metabolism. Interestingly, brain cells are
fastidious to culture and are weakly adherent on standard
culture vessels. These cells prefer vessels with coatings such as
poly-d-lysine, poly-ornithine, and laminin. Many researchers
self-coat vessels to enhance neural cell attachment, a
time-consuming process which may result in lot-to-lot and
plate-to-plate inconsistencies, often leading to experimental
rework. Surface coatings have profound effects on neural
cell morphology and function and influence the formation of
neurite outgrowths and branches, a hallmark of neural cell
differentiation. This application note reports that
BD PureCoat™ amine supports both neural cell
attachment and differentiation.
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Cell attachment, growth, and cell-to-cell interactions on a
surface or extracellular matrix substrate are highly complex
events involving cell adhesion molecules such as selectins,
integrins or cadherins. Another factor governing the growth
of cells is the composition of the culture medium, especially
serum which not only provides the necessary nutrients for
cells but also influences cell attachment. Serum is known
to contain many extracellular matrix proteins well known
for enhancing cell attachment. However, there are known
limitations to serum in culture medium. Apart from being
expensive, it can interfere with specific assays and introduce
variability due to lot-to-lot inconsistencies and the presence
of undefined components. Therefore, reducing or eliminating
serum in growth media is highly desirable and helps to reduce
costs involved in cell culture. However, many cell types do
not fare well in the absence of serum because of loss of
cell attachment. BD PureCoat™ surfaces allow robust cell
attachment and therefore are amenable tools to culturing cells
in reduced serum and/or serum-free medium. Enhanced cell
attachment on BD PureCoat surfaces is also beneficial for
recovery of cells from a freeze-thaw cycle. More importantly,
cells cultured on BD PureCoat surfaces remain functional
for a variety of assays including
in vitro cytoxicity and
transfection-based assays.
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