QuantiFluor RNA System from Promega

Product Specs
ItemQuantiFluor RNA System
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE3310
Quantity1ml
Molecular Weight~89kDa

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Targeting Extracellular RNA Mitigates Hepatic Lipotoxicity and Liver Injury in NASHCellsJuly 24, 2023Archana Tewari, Sangam Rajak, Sana Raza, Pratima Gupta, Bandana Chakravarti, Jyotika Srivastava, Chandra P. Chaturvedi, Rohit A. Sinhaand PA + RNase 1-treated HepG2 cells were analyzed using an eRNA quantitation kit (Promega, Cat No: E3310 QuantiFluor® RNA System, Madison, WI, USA), according to the manufacturer’s protocol, using a multimode

(2) TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseasesRedox biologyJuly 1, 2023Kim J, Lim J, Yoo ID, Park S, Moon JS.Maxwell® RSC Instrument (AS4500, Promega). Amounts of RNA were measured with QuantiFluor® RNA System (E3310, Promega) using Quantus™ Fluorometer (E6150, Promega). For each sample, 50 ng of total RNA was hybridized

(3) Protocol for analyzing intact mRNA poly(A) tail length using nanopore direct RNA sequencingSTAR ProtocolsJune 1, 2023Koichi Ogami, Yuka Oishi, Shin-ichi Hoshinocommercial assays,Critical commercial assays,Critical commercial assays ,, QuantiFluor RNA System,Promega,E3310 ,, Deposited data,Deposited data,Deposited data ,, dRNA-seq,"Ogami et al., 2022",GEO: GSE137807 ,, Experimental models

(4) Immune Profiling Panel Gene Set Identifies Critically Ill Patients With Low Monocyte Human Leukocyte Antigen-DR Expression: Preliminary Results From the REAnimation Low Immune Status Marker (REALISM) StudyCritical Care MedicineJune 1, 2023Estelle Peronnet, Sophie Blein, Fabienne Venet, Elisabeth Cerrato, Aurore Fleurie, Jean-François Llitjos, Louis Kreitmann, Gabriel Terraz, Filippo Conti, Morgane Gossez, et al.(AX2420; Promega Corporation, Madison, WI). RNA concentration was determined using QuantiFluor RNA system (E3310; Promega Corporation) on the GloMax Discover Microplate Reader (Promega). RNA integrity was assessed

(5) Metagenomic sequencing, molecular characterization, and Bayesian phylogenetics of imported type 2 vaccine-derived poliovirus, Spain, 2021Frontiers in Cellular and Infection MicrobiologyMay 2, 2023Maria Dolores Fernandez-Garcia, Martin Faye, Francisco Diez-Fuertes, Antonio Moreno-Docón, Maria Dolores Chirlaque-López, Ousmane Faye, Maria CabrerizoInc., USA) from a total concentration of 10 ng of RNA quantified using the QuantiFluor RNA System (E3310, Promega). Both libraries were sequenced using an Illumina MiSeq Reagent Kit v2 Nano on a MiSeq sequencer

(6) N-acetylcysteine overcomes NF1 loss-driven resistance to PI3Kα inhibition in breast cancerCell Reports MedicineApril 18, 2023Priska Auf der Maur, Marcel P. Trefny, Zora Baumann, Milica Vulin, Ana Luisa Correia, Maren Diepenbruck, Nicolas Kramer, Katrin Volkmann, Bogdan-Tiberius Preca, Pedro Ramos, et al.8.6-10 (average 9.7). RNA was quantified by Fluorometry using the QuantiFluor RNA System (Promega #E3310). Library preparation was performed, starting from 200 ng total RNA. The library was prepared with the

(7) A cell-type-specific alternative splicing regulator shapes synapse properties in a trans-synaptic mannerCell reportsMarch 28, 2023Traunmüller L, Schulz J, Ortiz R, Feng H, Furlanis E, Gomez AM, Schreiner D, Bischofberger J, Zhang C, Scheiffele P.analysis. RNA concentration was determined by Fluorometry using the QuantiFluor RNA System (Promega #E3310) and 20ng of RNA was reverse transcribed for analysis of marker enrichment by quantitative PCR. Only

(8) Natalizumab in cerebrospinal fluid and breastmilk of patients with multiple sclerosisTherapeutic Advances in Neurological DisordersJanuary 30, 2023Ilaria Callegari, Mika Schneider, Vera Aebischer, Margarete M. Voortman, Undine Proschmann, Tjalf Ziemssen, Raija Lindberg, Bettina Fischer-Barnicol, Michael Khalil, Ludwig Kappos, et al.high quality and consistency. RNA quantified by fluorometry using the QuantiFluor RNA System (Promega, E3310). Library preparation was performed, starting from 70 ng total RNA, using the TruSeq Stranded mRNA Library

(9) Multiomic profiling of the acute stress response in the mouse hippocampusNature CommunicationsDecember 1, 2022Lukas M. von Ziegler et al.Thermo Fisher Scientific) and a NanoDrop 3300 microvolume fluorospectrometer, or with the Quantifluor RNA System (E3310, Promega) and a DS-11 Series Spectrophotometer / Fluorometer (DeNovix). The integrity of the RNA was

(10) Prime-seq, efficient and powerful bulk RNA sequencingGenome BiologyDecember 1, 2022Aleksandar Janjic et al.MicroPrep Kit (Zymo, R2062). RNA concentrations were measured using the QuantiFlour RNA Dye (Promega, E3310) and 8 ng of total RNA were added per well. For the experiment estimating the impact of amplification

(11) Stimulus-specific remodeling of the neuronal transcriptome through nuclear intron-retaining transcriptsThe EMBO journalNovember 2, 2022Mazille M, Buczak K, Scheiffele P, Mauger O.RNA ScreenTape (Agilent, 5067‐5576) and quantified by Fluorometry using the QuantiFluor RNA System (Promega, E3310). Library preparation was performed, starting from 200 ng total RNAs, using the TruSeq Stranded mRNA

(12) Characterization of miRNAs in Milk Small Extracellular Vesicles from Enzootic Bovine Leukosis CattleInternational Journal of Molecular SciencesSeptember 15, 2022Fumi Tsukada, Shigeo Takashima, Yoshiko Wakihara, Yuji O. Kamatari, Kaori Shimizu, Ayaka Okada, Yasuo InoshimaAgilent Technologies) and concentration of extracted RNA was determined using a QuantiFluor RNA System (E3310, Promega). Next, to evaluate the utility of miRNA biomarker candidates selected using microarray analysis,

(13) Improved Bladder Tumor RNA Isolation from Archived Tissues Using Methylene Blue for Normalization, Multiplex RNA Hybridization, Sequencing and SubtypingInternational Journal of Molecular SciencesSeptember 6, 2022Stefanie A. Köhler et al.and solubilized in DEPC treated dH2O and then quantified using the QuantiFluor® RNA System (Promega, #E3310) and Nanodrop 2000 (Thermo Scientific). RNA quality, like the RNA Integrity Number (RIN) and the DV200

(14) Comprehensive Characterization of Human Lung Large Cell Carcinoma Identifies Transcriptomic Signatures with Potential Implications in Response to ImmunotherapyJournal of Clinical MedicineMarch 9, 2022Javier Ramos-Paradas et al.the manufacturer’s instructions. RNA quantification was performed with the Quantifluor RNA system (#E3310, Promega, Madrid, Spain), with an average yield of 141.09 ng/μL obtained, again exceeding the amounts

(15) Fms-like tyrosine kinase 3 is a regulator of the cardiac side population in miceLife Science AllianceMarch 1, 2022Giacomo Della Verde, Michika Mochizuki, Vera Lorenz, Julien Roux, Lifen Xu, Leandra Ramin-Wright, Otmar Pfister, Gabriela M Kusterconcentration was assessed using a Quantus Fluorometer (#E6150; Promega) and QuantiFluor RNA System (#E3310; Promega). Library preparations and RNA-seq were performed at the Quantitative Genomics Facility at

(16) Undetected αKlotho in serum is associated with the most aggressive phenotype of breast cancerMolecular and Clinical OncologyFebruary 25, 2022Bruna Cristina Borges, Pedro Augusto Do Amaral, Luiz Ricardo Soldi, Victor Luigi Costa Silva, Fernanda Carvalho De Souza, Felipe Andrés Cordeiro Da Luz, Rogério Agenor De Araújo, Marcelo José Barbosa Silvaup to 1 h. RNA and cDNA were quantified with GoScript™ RNA System and ssDNA System kits (cat. nos. E3310 and E3190; Promega Corporation) using a Quantus™ Fluorometer (Promega Corporation). All assays were

(17) ALK, NUT, and TRK Do Not Play Relevant Roles in Gastric Cancer—Results of an Immunohistochemical Study in a Large SeriesDiagnostics (Basel)February 7, 2022Marie-Isabelle Glückstein, Sebastian Dintner, Silvia Miller, Dmytro Vlasenko, Gerhard Schenkirsch, Abbas Agaimy, Bruno Märkl, Bianca GrosserUSA, AS1260) and fluorometrically quantified with QuantiFluor® RNA System (Promega, Madison, WI, USA, E3310). For long-term storage, the samples were kept at −80 °C. Library preparation and next-generation sequencing:

(18) Adaptation of the emerging pathogenic yeast Candida auris to high caspofungin concentrations correlates with cell wall changesVirulenceDecember 31, 2021Violeta Lara-Aguilar, Cristina Rueda, Irene García-Barbazán, Sarai Varona, Sara Monzón, Pilar Jiménez, Isabel Cuesta, Ángel Zaballos, Óscar Zaragozaof the libraries was estimated using Agilent 2100 Bioanalyzer and QuantiFluor® RNA System (Promega, E3310). A 4 nM pool containing equimolar fractions from each library was prepared, and 2 × 75 paired-end sequencing

(19) Clinicopathological and Molecular Characteristics of Macroscopically Yellowish-Colored Chromophobe Renal Cell Carcinoma Compared to Non-Yellowish-Colored Chromophobe Renal Cell CarcinomaClinical PathologyDecember 12, 2021Fumiyoshi Kojima et al.evaluated using a Quantus fluorometer (Promega Corporation, Madison, WI, USA) with QuantiFluor RNA Dye (E3310, Promega Corporation, Madison, WI, USA). Library construction and cDNA synthesis were performed using

(20) Tnfrsf4-expressing regulatory T cells promote immune escape of chronic myeloid leukemia stem cellsJCI insightDecember 8, 2021Hinterbrandner M, Rubino V, Stoll C, Forster S, Schnüriger N, Radpour R, Baerlocher GM, Ochsenbein AF, Riether C.(Agilent Technologies) and quantified by fluorometry using the Quantifluor RNA System Kit (catalog E3310, Promega) on a Quantus Fluorometer Instrument (Promega). Library preparation was performed from total

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QuantiFluor RNA System from Promega

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QuantiFluor RNA System from Promega

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