Description
KEY FEATURES AND BENEFITS
- Fast and easy processing using rapid spin-column format
- All columns for RNA purifi cation and protein purification
provided
- Sequentially isolate nucleic acids and proteins from a
single lysate: no need to split the lysate
- Isolate all sizes of RNA, from large mRNA and rRNA
down to microRNA (miRNA)
- No phenol or chloroform extractions
- Isolate high quality total RNA
- High yields of isolated proteins
KIT SPECIFICATIONS:
Column Binding Capacity ......................................50μ g RNA
200μ g for protein
Maximum Column Loading Volume ................600μ L
Size of RNA Purified ................................................All sizes, including small RNA (<200 nt)
Maximum Amount of Starting Material
Animal Cells ................................................................ 3 x 106 cells
Animal Tissues ..........................................................25mg
Blood .................................................................................100μ L
Bacteria .......................................................................... 1 x 109 cells
Yeast .................................................................................. 1 x 108 cells
Fungi .................................................................................50mg
Plant Tissues ...............................................................50mg
Time to complete purification process ........30 minutes for 10 purifications
Average Yields from RNA Purification
HeLa Cells (1 x 106 cells) ......................................15μ g RNA
HeLa Cells (1 x 106 cells) ......................................150μ g protein