Prime-a-Gene Labeling System from Promega

Product Specs
ItemPrime-a-Gene Labeling System
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberU1100
Quantity30 reactions
ApplicationsLabeling

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) A Msp1-containing complex removes orphaned proteins in the mitochondrial outer membrane of T. bruceiLife Sci AllianceAugust 16, 2023Markus Gerber, Ida Suppanz, Silke Oeljeklaus, Moritz Niemann, Sandro Käser, Bettina Warscheid, André Schneider, Caroline E Dewar,Thermo Fisher Scientific,# A-11008,"IFA (1:1,000)" Commercial assay or kit,Prime-a-Gene labelling kit,Promega,U1100,Radioactive labelling of Northern probes Commercial assay or kit,EZView Red Anti-c-myc affinity gel,Sigma-Aldrich,E6654,CoIP Commercial

(2) The telomerase reverse transcriptase elongates reversed replication forks at telomeric repeatsScience AdvancesMarch 22, 2023Armela Huda, Hiroshi Arakawa, Giulia Mazzucco, Martina Galli, Valentina Petrocelli, Stefano Casola, Lu Chen, Ylli Doksanimembrane (GE Healthcare, RPN203). Probes were labeled with the Prime-a-Gene Labeling system (Promega, U1100) using [α-32P]deoxycytidine triphosphate (dCTP) (3000 Ci/μmol; PerkinElmer, 1300000327). For the TSQ1

(3) TYMV and TRV infect Arabidopsis thaliana by expressing weak suppressors of RNA silencing and inducing host RNASE THREE LIKE1PLOS PathogensJanuary 25, 2023Hayat Sehki, Agnès Yu, Taline Elmayan, Hervé Vaucheret(Machery-Nagel) and radiolabeled dCTP-P32 were incorporated with a Prime-a-gene Labeling System kit (U1100, Promega). Oligonucleotide probes were ordered from GenoScreen and radiolabeled dATP-P32 were incorporated

(4) Enrichment of centromeric DNA from human cellsPLOS GeneticsJuly 19, 2022Riccardo Gamba et al.probe for Southern was produced by apha-32P-dCTP-labelling (Prime-a-Gene Labeling System, Promega cat #U1100) of a ~300 bp PCR product obtained with primers 5′-CAGAAACTTCTTTGTGATGTGTGC-3′ and 5’-GTTTTTATGGGAAGATATTTCCT-3’

(5) Comparative analysis of potential broad-spectrum neuronal Cre driversWellcome Open ResearchJuly 8, 2022Katie M Paton, Jim Selfridge, Jacky Guy, Adrian BirdNcoI–BamHI fragment of the 3′UTR of Mecp2 ( Guy et al., 2001)). The Prime-a-Gene Labelling System (Promega U1100) was used to label the probe with [α32]dCTP. Membranes were washed briefly with room temperature 2×

(6) Nuclear and cytoplasmic RNA exosomes and PELOTA1 prevent miRNA-induced secondary siRNA production in ArabidopsisNucleic Acids ResearchFebruary 22, 2022Maria L Vigh, Simon Bressendorff, Axel Thieffry, Laura Arribas-Hernández, Peter Brodersenhybridized O/N at 65°C to radioactively labeled 3′-CF probes produced with Prime-a-gene labeling kit (#U1100, Promega). The membranes were washed 3 times in 2× SSC (0.3 M NaCl, 30 mM sodium citrate), 0.1% SDS

(7) SETDB1/NSD-dependent H3K9me3/H3K36me3 dual heterochromatin maintains gene expression profiles by bookmarking poised enhancersMolecular cellFebruary 17, 2022Barral A, Pozo G, Ducrot L, Papadopoulos GL, Sauzet S, Oldfield AJ, Cavalli G, Déjardin J.Kit,Illumina,Cat#FC-131-1024 Luciferase Assay System,Promega,Cat#E1500 Prime-a-Gene® Labeling System,Promega,Cat#U1100 EpiTect Hi-C Kit,Qiagen,Cat#59971 MinElute PCR Purification Kit,Qiagen,Cat#28004 DNA Clean & Concentrator-5

(8) Innate, translation-dependent silencing of an invasive transposon in ArabidopsisEMBO reportsFebruary 3, 2022Oberlin S, Rajeswaran R, Trasser M, Barragán-Borrero V, Schon MA, Plotnikova A, Loncsek L, Nodine MD, Marí-Ordóñez A, Voinnet O.crosslinked (Pall & Hamilton, 2008). Probes from PCR products were radiolabeled using the Prime‐a‐Gene kit (#U1100, Promega) in the presence of [α‐32P]‐dCTP (Hartmann Analytic) and oligo probes were radiolabeled by

(9) Relative efficiency of split-marker versus double-crossover replacement protocols for production of deletion mutants in strain PH-1 of Fusarium graminearumFungal Genetics ReportsApril 29, 2021Sladana Bec, Gabdiel E. Yulfo-Soto, Lisa J. Vaillancourtgel-purified and labeled with radioactive isotope P32 using the Prime-a-gene labeling system (Promega, cat. #U1100). Between 1 and 5 µg of the genomic DNA isolated from the fungal strains was digested with 20 U of the

(10) The N-terminal BRCT domain determines MCPH1 function in brain development and fertilityCell Death & DiseaseFebruary 1, 2021Xiaoqian Liu et al.indicated probes after labelling with α-32P dCTP (Hartmann Analytic) using Prime-a-Gene® Labelling System (#U1100, Promega), as per manufacturer’s protocol and the Amersham NICK Columns (108–0855–02, GE healthcare

(11) Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene ExpressionMolecular & cellular proteomics : MCPJanuary 1, 2021Wu T, Nance J, Chu F, Fazzio TG.[α-32P]dCTP (PerkinElmer; 3000 Ci/mmol; 10 mCi/ml, BLU013H100UC) using Prime-a-Gene Labeling System (Promega; U1100). Unincorporated dCTP was removed by Bio-Spin P-30 columns (Bio-Rad; 7326231). Radioactivity was determined

(12) Location specific annealing of miR-122 and other small RNAs defines an Hepatitis C Virus 5′ UTR regulatory element with distinct impacts on virus translation and genome stabilityNucleic Acids ResearchSeptember 18, 2020Rasika D Kunden, Sarah Ghezelbash, Juveriya Q Khan, Joyce A WilsonGAPDH separately. The radioactive DNA probes used were prepared using Prime-a-Gene Labeling System kit (U1100, Promega, Madison, WI, USA), and radiolabeled dCTP (PerkinElmer, Boston, USA). The probes were generated

(13) PDM4, a Pentatricopeptide Repeat Protein, Affects Chloroplast Gene Expression and Chloroplast Development in Arabidopsis thalianaFrontiers in Plant ScienceAugust 11, 2020Xinwei Wang, Lirong Zhao, Yi Man, Xiaojuan Li, Li Wang, Jianwei Xiaolabeled with 32P. The labeled probes were obtained by using the Prime-a-Gene Labeling Kit (SGMB01-Promega-U1100, USA). The sequences of the primers were according to Du et al. (2017). All the analysis was performed

(14) Virus-host coexistence in phytoplankton through the genomic lensScience AdvancesApril 3, 2020Sheree Yau et al.BLU008H250UC) according to the manufacturer’s instructions (Prime-a-Gene kit, Promega, catalog no. U1100) for use as DNA probes. Dried gels were equilibrated in sufficient hybridization buffer [6× SSC, 5×

(15) Homologue replacement in the import motor of the mitochondrial inner membrane of trypanosomeseLifeFebruary 27, 2020Corinne von Känel, Sergio A Muñoz-Gómez, Silke Oeljeklaus, Christoph Wenger, Bettina Warscheid, Jeremy G Wideman, Anke Harsman, Andre Schneiderreagent,MCP12ΔI-myc (plasmid),PMID: 27991487,,, Commercial assay or kit,Prime-a-Gene labelling kit,Promega,U1100,Radioactive labelling of Northern probes,Radioactive labelling of Northern probes Commercial assay

(16) SUMO-Chain-Regulated Proteasomal Degradation Timing Exemplified in DNA Replication InitiationMolecular CellNovember 21, 2019Ivan Psakhye, Federica Castellucci, Dana Branzei28106 ProbeQuant G-50 micro columns,GE Healthcare,Cat# 28903408 Prime-a-Gene labeling system,Promega,Cat# U1100 "Invitrogen Bolt 4-12% Bis-Tris Plus Gels, 15-well",Thermo Fisher Scientific,Cat# NW04125BOX "L-Arginine:HCl

(17) The m⁶A pathway protects the transcriptome integrity by restricting RNA chimera formation in plantsLife science allianceJune 1, 2019Pontier D, Picart C, El Baidouri M, Roudier F, Xu T, Lahmy S, Llauro C, Azevedo J, Laudié M, Attina A, et al.GeneClean Turbo Kit (Cat. No. 111102400; MP Biomedicals), and labeled with the Prime-a-Gene Labeling System (Cat. No. U1100; Promega) according to the manufacturer's instructions. The hybridization was performed in the PerfectHyb

(18) Simplified Transformation of Ostreococcus tauri Using Polyethylene GlycolGenes (Basel)May 26, 2019Frédéric Sanchez, Solène Geffroy, Manon Norest, Sheree Yau, Hervé Moreau, Nigel Grimsley(Perkin-Elmer ref BLU008H250UC) according to the manufacturer’s instructions (Prime-a-Gene kit, Promega ref U1100) for use as a DNA probe. Dried gels were equilibrated in hybridisation buffer (6 × SSC, 5 × Denhardt’s

(19) Toxicity of overexpressed MeCP2 is independent of HDAC3 activityGenes & DevelopmentDecember 1, 2018Martha V. Koerner et al.chromosome 11: 104,142,739–104,143,549) were labeled using the Prime-a-Gene labeling system (Promega, U1100) with α32-dCTP. Blots were washed twice for 30 min in Church wash (1% SDS, 20 mM Na2HPO4) and exposed

(20) RNase E cleavage shapes the transcriptome ofRhodobacter sphaeroidesand strongly impacts phototrophic growthLife Science AllianceAugust 1, 2018Konrad U Förstner, Carina M Reuscher, Kerstin Haberzettl, Lennart Weber, Gabriele Klugwas labeled with α-[32P]-dCTP (SRP-205; Hartmann Analytic) using the Prime-a-Gene Labeling System (U1100; Promega) as described in the manufacturer’s manual. Source data for the Northern blots are provided

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