Propidium Iodide Solution from BioLegend

Product Specs
ItemPropidium Iodide Solution
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number421301
ApplicationsFlow Cytometry (FC)
Quantity2 mL
FormatPhosphate-buffered saline, pH 7.2, containing 0.09% sodium azide
Concentration0.50 mg/mL

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Decreased melanoma CSF-1 secretion by Cannabigerol treatment reprograms regulatory myeloid cells and reduces tumor progressionOncoImmunologyDecember 31, 2023Iris Wyrobnik, Miryam Steinberg, Anat Gelfand, Ronen Rosenblum, Yara Eid Mutlak, Liron Sulimani, Shiri Procaccia, Yishai Ofran, Hila Novak-Kotzer, David Meiricells stained for Annexin V (BioLegend, Cat# 640941, 1:100) and propidium iodide (PI, BioLegend, Cat# 421301, 1:400) with indicated treatments. Stained cells were analyzed by BD FlowJo™ software version x.0.7

(2) lncRNA GPRC5D-AS1 as a ceRNA inhibits skeletal muscle aging by regulating miR-520d-5pAging (Albany NY)December 9, 2023Miao Yu, Xiuting He, Ting Liu, Jie Li(Beyotime, China; ST578) at room temperature for 30 min and stained with 50 μg/ml PI (Biolegend, USA; 421301) at room temperature for 30 min. Cell cycle was tested on flow cytometry. Dual-luciferase reporter

(3) The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myelomaMolecular oncologyDecember 1, 2023Luo G, Aldridge K, Chen T, Aslot V, Kim BG, Han EH, Singh N, Li S, Xiao TS, Sporn MB, et al.stained with Annexin V (Catalog #640906; Biolegend, San Diego, CA, USA) and propidium iodide (Catalog #421301; Biolegend) after 24 h and analyzed according to the manufacturer's instruction. Experiments were done

(4) 3D-printed scaffold harboring copper ions combined with near-infrared irradiation for local therapy of canceriScienceOctober 20, 2023Hao Wei, Dong Chen, Bin Han, Peng Li, Hao Jia, Lizhang Zhu, Hao Yang, Deren Lan, Wei Wei, Haibo Chen, et al.double-stained in the dark with 5 μM of calcein-AM (425201, Biolegend, USA) and 5 μM of propidium iodide (PI) (421301, Biolegend) for 20 min. Then, the cells or organoids were washed twice with PBS, and observed with a

(5) Erucic acid improves the progress of pregnancy complicated with systemic lupus erythematosus by inhibiting the effector function of CD8+ T cellsMedComm (2020)October 1, 2023Yanling Chang, Meng Jiang, You Wang, Qiong Fu, Sihan Lin, Jiayue Wu, Wen Di(PRID: AB_314470), anti‐CD95 (PRID: AB_314544), anti‐CD36 (PRID: AB_1575025), and propidium iodide (#421301, Biolegend). The following antibodies against mouse proteins were utilized: anti‐CD45 (PRID: AB_893340),

(6) Preclinical Characterization and Phase I Trial Results of INBRX-109, A Third-Generation, Recombinant, Humanized, Death Receptor 5 Agonist Antibody, in ChondrosarcomaClinical Cancer ResearchAugust 15, 2023Vivek Subbiah, Sant P. Chawla, Anthony P. Conley, Breelyn A. Wilky, Anthony Tolcher, Nehal J. Lakhani, David Berz, Vasily Andrianov, William Crago, Monica Holcomb, et al.resuspended in 100 μL FACS buffer containing a 1:2,000 dilution of propidium iodide (BioLegend, Catalog No. 421301) and respective antibodies. AF647-conjugated antihuman crystallizable fragment (Fc) antibody (1:1,000;

(7) In silico enhancer mining reveals SNS-032 and EHMT2 inhibitors as therapeutic candidates in high-grade serous ovarian cancerBritish Journal of CancerJuly 27, 2023Marcos Quintela, David W. James, Jetzabel Garcia, Kadie Edwards, Lavinia Margarit, Nagindra Das, Kerryn Lutchman-Singh, Amy L. Beynon, Inmaculada Rioja, Rab K. Prinjha, et al.Apoptosis staining was performed using Annexin V-FITC (640906, Biolegend; CA, US) and Propidium iodide (PI, 421301, Biolegend) as per manufacturer’s instructions, which included the use of Annexin V Binding Buffer (422201,

(8) Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cellsCell reportsJuly 25, 2023Qian F, Nettleford SK, Zhou J, Arner BE, Hall MA, Sharma A, Annageldiyev C, Rossi RM, Tukaramrao DB, Sarkar D, et al.,Thermo Fisher,Cat# 23224 Poly(ethyleneimine),Supelco,Cat# P3143 Propidium iodide solution,BioLegend,Cat# 421301 Prostaglandin D2,Cayman Chemical Co.,Cat# 12010 "Prostanoid CRTH2 (human) membrane preparation, in

(9) High-Dimensional Single-Cell Multimodal Landscape of Human Carotid AtherosclerosismedRxivJuly 20, 2023Alexander C. Bashore, Hanying Yan, Chenyi Xue, Lucie Y. Zhu, Eunyoung Kim, Thomas Mawson, Johana Coronel, Allen Chung, Sebastian Ho, Leila S. Ross, et al.suspensions were stained with Calcein AM (Thermo Fisher Scientific C34852) and Propidium Iodide (BioLegend 421301), filtered through a 40-μM cell strainer and immediately brought to the flow cytometry core for sorting.

(10) Chromatin accessibility dynamics of neurogenic niche cells reveal defects in neural stem cell adhesion and migration during agingNature AgingJuly 13, 2023Robin W. Yeo, Olivia Y. Zhou, Brian L. Zhong, Eric D. Sun, Paloma Navarro Negredo, Surag Nair, Mahfuza Sharmin, Tyson J. Ruetz, Mikaela Wilson, Anshul Kundaje, et al.12587-010), 1% penicillin–streptomycin–glutamine (Gibco, 10378-016) with propidium iodide (BioLegend, 421301; 1:5,000 dilution) for live/dead staining. Cells were kept on ice during all subsequent steps. Due to

(11) The circadian clock is disrupted in pancreatic cancerPLoS geneticsJune 1, 2023Schwartz PB, Nukaya M, Berres ME, Rubinstein CD, Wu G, Hogenesch JB, Bradfield CA, Ronnekleiv-Kelly SM.were grown to confluence, fixed with 70% ethanol, and stained with 50 μg/mL propidium iodide (Catalog: 421301, BioLegend, San Diego, CA) and 100 μg/mL RNAse A (Catalog: FEREN0531, Thermo Fisher). Samples (n = 3

(12) miR-216b-5p promotes late apoptosis/necroptosis in trastuzumab-resistant SK-BR-3 cellsTurkish Journal of BiologyMay 23, 2023İŞTAR BURCU DOLAPÇI, SENEM NOYAN, AYŞEGÜL YÜCEL POLAT, HAKAN GÜRDAL, BALA GÜR DEDEOĞLUin a concentration of 1 × 106 cells/mL. Five μL Annexin V-FITC (640906, BioLegend) and five μL PI (421301, BioLegend) (0.5 μg/μL) were added and the cells were incubated in dark for 15 min. After the incubation

(13) CD317 maintains proteostasis and cell survival in response to proteasome inhibitors by targeting calnexin for RACK1-mediated autophagic degradationCell death & diseaseMay 20, 2023Cheng J, Zhang G, Deng T, Liu Z, Zhang M, Zhang P, Adeshakin FO, Niu X, Yan D, Wan X, et al.bovine serum (FBS) were purchased from HyClone, L-glutamine from Gibco, Propidium Iodide (PI) Solution (421301) from Biolegend (CA, USA), Annexin V-FITC/PI apoptosis detection kit (FA101-02) from TransGen Biotech

(14) Lipid droplets and peroxisomes are co-regulated to drive lifespan extension in response to mono-unsaturated fatty acidsNature Cell BiologyMay 1, 2023Katharina Papsdorf, Jason W. Miklas, Amir Hosseini, Matias Cabruja, Christopher S. Morrow, Marzia Savini, Yong Yu, Carlos G. Silva-García, Nicole R. Haseley, Luke Meraz Murphy, et al.before69. Briefly, plates were prepared by mixing 2.5 µl of 0.5 mg ml−1 propidium iodide (BioLegend, 421301) with 50 µl of HT115 bacteria and adding this mix onto RNAi plates. Propidium iodide plates were prepared

(15) ERK Hyperactivation Serves as a Unified Mechanism of Escape in Intrinsic and Acquired CDK4/6 Inhibitor Resistance in Acral Lentiginous MelanomaResearch SquareApril 21, 2023Vito Rebecca, Kasturee Jagirdar, Marie Portuallo, Meihan Wei, Matthew Wilhide, Jeremy Bravo, Bailey Robertson, Gretchen Alicea, Crsytal Aguh, Min Xiao, et al.resuspended in PBS, treated with RNase A solution and stained with propidium iodide (0.5 mg/mL, BioLegend, 421301) Cell cycle analysis was performed on Cytek™ NL-3000 and data were analyzed using FlowJo Software. Cell

(16) Lysosomal lipid peroxidation regulates tumor immunityJournal of Clinical InvestigationApril 17, 2023Monika Bhardwaj, Jennifer J. Lee, Amanda M. Versace, Sandra L. Harper, Aaron R. Goldman, Mary Ann S. Crissey, Vaibhav Jain, Mahendra Pal Singh, Megane Vernon, Andrew E. Aplin, et al.Alexa Fluor 647 goat anti-rabbit second antibody (Invitrogen), and propidium iodide (PI) (Biolegend, 421301). Stained samples were acquired on a BD LSRII flow cytometer using 710/50 Blue and 670/30 Red to capture

(17) Identification of the mouse Kupffer cell receptors recognizing pneumococcal capsules by affinity screeningSTAR ProtocolsMarch 1, 2023Haoran An, Xianbin Tian, Yijia Huang, Jing-Ren ZhangM0202S HBSS,Corning,Cat# 21-022-CV PBS,Corning,Cat# 21-040-CVC RBC lysis buffer (10×),BioLegend,Cat# 421301 Halt Protease and Phosphatase Inhibitor Cocktail,Thermo Scientific,Cat# 78442 DMTMM,Sigma-Aldrich,Cat#

(18) Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathwayNature immunologyMarch 1, 2023Tanaka T, Okuda H, Isonishi A, Terada Y, Kitabatake M, Shinjo T, Nishimura K, Takemura S, Furue H, Ito T, et al.APC–streptavidin (1:200, 405207, BioLegend). To exclude dead cells from analysis, cells were stained with PI (421301, BioLegend), 7-AAD (559925, BD Biosciences) or Fixable Viability Stain 700 (564997, BD Biosciences).

(19) Dipeptidylpeptidase 4 promotes survival and stemness of acute myeloid leukemia stem cellsCell reportsFebruary 28, 2023Wang C, Nistala R, Cao M, Pan Y, Behrens M, Doll D, Hammer RD, Nistala P, Chang HM, Yeh ETH, et al.AB_2562057 Annexin V,Biolegend,Cat#640941; RRID: AB_2616657 7-AAD,Biolegend,Cat#420404 PI,Biolegend,Cat#421301 Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains pLentiLoxp3.7,"Addgene,

(20) Circadian regulator CLOCK promotes tumor angiogenesis in glioblastomaCell reportsFebruary 28, 2023Pang L, Dunterman M, Xuan W, Gonzalez A, Lin Y, Hsu WH, Khan F, Hagan RS, Muller WA, Heimberger AB, et al.stained with Apotracker (1:10 dilution) and then 5 μL propidium iodide (PI) solution (BioLegend, # 421301) was added to label late apoptotic and necrotic cells. After washing with PBS, FITC and PI signals were

Write a Review

(1)

1/20/2021PI is a Simple but Effective live/dead Stain Anonymous ReviewTo further characterize cell populations it is best to initially gate on live cells. in this experiment I used PI as my live/dead stain.

Propidium Iodide Solution from BioLegend

Description

BioLegend

Citations (174)

Reviews

Propidium Iodide Solution from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map