E.coli Poly (A) Polymerase from New England Biolabs

Product Specs
ItemE.coli Poly (A) Polymerase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0276S
Quantity100 units
Concentration20,000 units/ml
ApplicationsFunctional Genomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 50 µl volume in 10 minutes at 37°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Targeted rRNA depletion enables efficient mRNA sequencing in diverse bacterial species and complex co-culturesmSystemsDecember 21, 2023Kellie A. Heom, Chatarin Wangsanuwat, Lazarina V. Butkovich, Scott C. Tam, Annette R. Rowe, Michelle A. O'Malley, Siddharth S. Deyblocking primer mix, 0.1 µL 10 mM ATP, and 0.1 µL E. coli poly-A polymerase (New England Biolabs, Cat. # M0276S). The samples were incubated at 37°C for 10 min. In the “No depletion” and “RNase H” libraries, 1 µL

(2) A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma bruceiNature CommunicationsNovember 20, 2023K. Shanmugha Rajan, Hava Madmoni, Anat Bashan, Masato Taoka, Saurav Aryal, Yuko Nobe, Tirza Doniger, Beathrice Galili Kostin, Amit Blumberg, Smadar Cohen-Chalamish, et al.selection of large RNA). ~15 µg of the size selected RNA was later treated with poly-A polymerase (NEB M0276S) for 30 min at 37 oC as recommended by manufacturer’s protocol (100 µl protocol), and then poly-A +

(3) An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activityNature Chemical BiologyNovember 1, 2023Tong Wu, Chang Liu, Siyuan Zou, Ruitu Lyu, Bowei Yang, Hao Yan, Minglei Zhao, Weixin Tangsubjected to poly(A) tailing using 5 units (1 μL) of E. coli Poly(A) Polymerase (New England Biolabs, M0276S) following manufacturer’s protocol. RNA was then purified using the RNA Clean & Concentrator kit (Zymo Research

(4) FTO-mediated LINC01134 stabilization to promote chemoresistance through miR-140-3p/WNT5A/WNT pathway in PDACCell Death & DiseaseNovember 1, 2023Jin Lu, Yongsheng Yang, Xiangliang Liu, Xiao Chen, Wei Song, Zefeng LiuRevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, #K1622, city, state, USA) and poly(A) Polymerase Reaction Buffer (NEB, M0276s, city, state, USA) to synthesize complementary DNA (cDNA). Real-time qRT-PCR analysis was performed

(5) The intrinsically disordered region of eIF5B stimulates IRES usage and nucleates biological granule formationCell ReportsOctober 19, 2023Meghan T. Harris, Michael T. MarrArsenite,Fisher,714216 Vaccinia Virus Capping enzyme,New England Biolabs,M2080 E. coli poly(A)polymerase,New England Biolabs,M0276S T3 polymerase,New England Biolabs,M0378S Rabbit Reticulocyte Lysate,Green Hectares, 5x Passive Lysis

(6) mRNA vaccine quality analysis using RNA sequencingNature CommunicationsSeptember 21, 2023Helen M. Gunter, Senel Idrisoglu, Swati Singh, Dae Jong Han, Emily Ariens, Jonathan R. Peters, Ted Wong, Seth W. Cheetham, Jun Xu, Subash Kumar Rai, et al.E. coli Poly(A) Polymerase. Briefly, up to 10 µg of RNA was incubated with NEB E. coli Poly(A) Polymerase (M0276) and 1 mM ATP for 1.5 min. The reaction was stopped using EDTA (to a final concentration of 10 mM),

(7) Detecting intragenic trans-splicing events from non-co-linearly spliced junctions by hybrid sequencingNucleic Acids ResearchAugust 25, 2023Yu-Chen Chen, Chia-Ying Chen, Tai-Wei Chiang, Ming-Hsien Chan, Michael Hsiao, Huei-Mien Ke, Isheng Jason Tsai, Trees-Juen Chuangexperiment, 1 μg of total RNA was subjected to polyA tailing using E.coli Poly(A) polymerase (NEB, Cat. M0276), following the manufacturer's protocol. The resulting mixture was then purified by the Trizol method.

(8) In vitro Analysis of Stalled Ribosomes Using Puromycin IncorporationBio ProtocAugust 20, 2023MaKenzie R. Scarpitti, Michael G. Kearse(New England Biolabs, catalog number: M0303L)E. coli Poly(A) polymerase (New England Biolabs, catalog number: M0276L)500 mM EDTA, pH 8.0 ULTROL grade (Millipore, catalog number: 324504)Ethanol, 200 proof (Decon Laboratories,

(9) Nanopore-based RNA sequencing deciphers the formation, processing, and modification steps of rRNA intermediates in archaeaRNAAugust 1, 2023Felix Grünberger, Michael Jüttner, Robert Knüppel, Sébastien Ferreira-Cerca, Dina GrohmannConcentrator-5 Kit (ZYMO, R1013), RNAs were polyadenylated using the E. coli poly(A) polymerase (NEB, M0276) following a recently published protocol (Yan et al. 2018). Briefly, 5 µg RNA, 20 U poly(A) polymerase,

(10) Rubella virus tropism and single-cell responses in human primary tissue and microglia-containing organoidseLifeJuly 20, 2023Popova G, Retallack H, Kim CN, Wang A, Shin D, DeRisi JL, Nowakowski T.(New England Biolabs, M0207L). The resulting RNA was purified then polyadenylated (New England Biolabs, M0276S) and capped using Vaccinia Capping System (New England Biolabs, M2080S). This RNA was then introduced

(11) Translation re-initiation after uORFs does not fully protect mRNAs from nonsense-mediated decayRNAJune 1, 2023Paul J. Russell, Jacob A. Slivka, Elaina P. Boyle, Arthur H.M. Burghes, Michael G. KearseS1406L). Post-transcriptional polyadenylation was performed using E. coli poly(A) polymerase (NEB # M0276L). mRNAs were purified using the Zymo RNA Clean & Concentrator-25 (Zymo Research # R1018), eluted in

(12) Inhibition of PERK Kinase, an Orchestrator of the Unfolded Protein Response (UPR), Significantly Reduces Apoptosis and Inflammation of Lung Epithelial Cells Triggered by SARS-CoV-2 ORF3a ProteinBiomedicinesMay 30, 2023Panagiotis Keramidas, Eleni Papachristou, Rigini M. Papi, Aglaia Mantsou, Theodora Choli-PapadopoulouUSA). Poly-A tailing of ORF3a’s and GFP’s mRNA was carried out using E. coli Poly(A) Polymerase (NEB# M0276, New England Biolabs, Ipswich, MA, USA). Synthesized mRNAs were purified using a spin column-based method

(13) Rapid production and free distribution of a synthetic RNA material to support SARS-CoV-2 molecular diagnostic testingBiologicalsMay 3, 2023Megan H. Cleveland, Erica L. Romsos, Carolyn R. Steffen, Nathan D. Olson, Stephanie L. Servetas, William G. Valiant, Peter M. Vallonetail was added to the fragments using an E. coli Poly(A) Polymerase (New England Biolabs, catalog #M0276S). Following poly(A) tailing, the fragments were bound to RNAclean XP beads (Beckman Coulter, Brea, CA,

(14) TUT4/7-mediated uridylation of a coronavirus subgenomic RNAs delays viral replicationCommunications BiologyApril 21, 2023Ankit Gupta, Yin Li, Shih-Heng Chen, Brian N. Papas, Negin P. Martin, Marcos Morgan1× E. coli poly(A) polymerase reaction buffer, ATP 1 mM, 0.5 µl of E. coli Poly(A) polymerase (NEB, M0276S), and 10 µg of purified in vitro transcribed RNA in a total volume of 20 µl. The reaction was incubated

(15) Multicellular, IVT-derived, unmodified human transcriptome for nanopore direct RNA analysisbioRxivApril 13, 2023Caroline A. McCormick, Stuart Akeson, Sepideh Tavakoli, Dylan Bloch, Isabel N. Klink, Miten Jain, Sara H. Rouhanifardthe Manufacturer's protocols. Polyadenylation was performed using E. coli Poly(A) Polymerase (NEB, M0276S) using 12 μg of input RNA, and EDTA was added to halt the reaction. The product was purified again using

(16) In-depth study of tomato and weed viromes reveals undiscovered plant virus diversity in an agroecosystemMicrobiomeMarch 28, 2023Mark Paul Selda Rivarez, Anja Pecman, Katarina Bačnik, Olivera Maksimović, Ana Vučurović, Gabrijel Seljak, Nataša Mehle, Ion Gutiérrez-Aguirre, Maja Ravnikar, Denis KutnjakPlant Kit (Invitrogen, USA), then ligated with poly-A sequences using E. coli Poly(A) polymerase (NEB #M0276, UK). Library preparation was done using the PCR-cDNA Barcoding kit (SQK-PCB109, version 10Oct2019,

(17) Hepatocellular Carcinoma Cell-Derived Exosomal miR-21-5p Induces Macrophage M2 Polarization by Targeting RhoBInternational journal of molecular sciencesFebruary 27, 2023Yu H, Pan J, Zheng S, Cai D, Luo A, Xia Z, Huang J.After total RNA extraction, a tailing reaction was performed using E. coli Poly(A) Polymerase (NEB# M0276, Ipswich, MA, USA) and incubated at 37 °C for 30 min. Reverse transcription was performed using miRNA

(18) Terminal hairpins improve protein expression in IRES-initiated mRNA in the absence of a cap and polyadenylated tailGene TherapyFebruary 24, 2023Victor Solodushko, Brian Foutypoly(A) (range from 75–200 adenosines) using E. coli Poly(A) Polymerase (New England BioLabs, NEB# M0276). The purity of mRNA and the length of poly(A) tails (if applicable) were confirmed by gel electrophoresis.

(19) Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct long-read sequencingNature CommunicationsJanuary 19, 2023Sepideh Tavakoli, Mohammad Nabizadeh, Amr Makhamreh, Howard Gamper, Caroline A. McCormick, Neda K. Rezapour, Ya-Ming Hou, Meni Wanunu, Sara H. Rouhanifardproduct was eluted in nuclease-free water and poly-A tailed using E. coli Poly(A) Polymerase (NEB, M0276). The product was purified once again using an RNA Cleanup Kit and adjusted to 500 ng polyA RNA in 9

(20) Nano3P-seq: transcriptome-wide analysis of gene expression and tail dynamics using end-capture nanopore cDNA sequencingNature MethodsJanuary 1, 2023Oguzhan Begik, Gregor Diensthuber, Huanle Liu, Anna Delgado-Tejedor, Cassandra Kontur, Adnan Muhammad Niazi, Eivind Valen, Antonio J. Giraldez, Jean-Denis Beaudoin, John S. Mattick, et al.Transcription Kit (Lucigen-ASF3507). Curlcake 2 was polyadenylated using Escherichia coli polyA Polymerase (NEB-M0276S). polyA-tailed RNAs were purified using RNAClean XP beads. The quality of the in vitro transcribed (IVT)

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